Investigations were carried out to explore the changes of muscle and liver fatty acid composition and content in Pseudosciaena crocea during the winter. Gas chromatography-mass spectrometry (GC-MS) was used to detect fatty acid composition and content of its muscle and liver under the condition of sea water temperature at 20 ℃, 16 ℃, 12 ℃, 10 ℃ and 8 ℃ respectively. The results show that the content of unsaturated fatty acid increases with the reduction of water temperature, especially the content of EPA[C20:5(n-3)], from 5.71% at 20 ℃ to 8.06% at 8 ℃ in muscle and from 6.76% at 20 ℃ to 8.44% at 8 ℃ in liver. Therefore, with no feeding condition during the winter, saturated fatty acid decreases and unsaturated fatty acid increases through transformation and metabolism of fatty acid by organism itself to keep cell membranes fluidity and against the cold.
Key words
Pseudosciaena crocea /
winter /
fatty acid
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] ITIO S, KAWAHARA S, KONDO H, et al. Changes in mitochondrial fatty acid composition following temperature acclimation of carp and their possible effects on F0F1-ATPase activity[J]. Fish Plysiology and Biochemistry, 2003,3(29):237-244.
[2] WANG Yan, HU Xian-cheng, HAN Qiang. Composition of fatty acids in starved and refeeding juvenile sea perch Lateolabrax Japonicas under different salinities[J]. Fisheries Science,2008,27(7):334-339.
王艳,胡先成,韩强.不同盐度条件下饥饿及恢复摄食鲈稚鱼脂肪酸的组成[J].水产科学,2008,27(7):334-339.
[3] GE Zhong-xian. Effects of low temperature stress on fatty acid composition and content of Oreochromis hornorum[D]. Zhanjiang: Guangdong Ocean University,2011.
葛仲显.低温胁迫对荷那龙罗非鱼脂肪酸组成与含量的影响[D].湛江:广东海洋大学,2011.
[4] WANG Zheng-fan. Qualitative and quantitative chromatographic[M]. Beijing: Chemical Industry Press, 2000:168-169.
汪正范.色谱定性与定量[M].北京:化学工业出版社,2000:168-169.
[5] LYONS J M, GRANHAM D, RAISON J K. Low temperature stress in corp plants: The role of the membrane[M]. New York: Academic Press,1970:33-56.
[6] FARKAS T, CSENGERI I, MAJOROS F, et al. Metabolism of fatty acids in fish: III. Combined effect of environmental temperature and diet on formation and deposition of fatty acids in the carp, Cyprinus carpio Linnaeus 1758[J]. Aquaculture, 1980,20(1):29-40.
[7] TONG Sheng-ying. Changes of fatty acid composition in carp during winter[J]. Journal of Fisheries of China, 1997,21(4):373-379.
童圣英.四种鲤科鱼类越冬时脂肪酸组成的变化[J].水产学报,1997,21(4):373-379.
[8] VAN D, THILLART G, MODDERKOLK J. The effect of acclimation temperature on the activation energies of state III respiration and on the unsaturation of membrane lipids of goldfish mitochondria[J]. Biochimica et Biophysica Acta, 1978,510(1):38-51.
[9] VAN D, THILLART G, BRUIN G. Influence of environmental temperature on mitochondrial membranes[J]. Biochimica et Biophysica Acta, 1981,640(2):439-447.
[10] WODTKE E. Temperature adaptation of biological membranes: The effects of acclimation temperature on the unsaturation of the main neutral and charged phospholipids in mitochondrial membranes of the carp (Cyprinus carpio L.)[J]. Biochimica et Biophysica Acta, 1981,640(3):698-709.
[11] ZHU Bang-ke, CAO Wen-xuan. Fatty acid composition and their changes during early life stage of silver carp Hypophthalmichthys molitrix[J]. Acta Hydrobiologica Sinica, 2002,26(2):130-135.
朱邦科,曹文轩.鲢早期发育阶段鱼体脂肪酸组成变化[J].水生生物学报,2002,26(2):130-135.
PDF(936 KB)
