仿刺参采苗板上细菌的分离与鉴定

卢晓平; 许艳蕊; 范秋苹; 王春蕾; 郝鲁江

doi:10.3969/j.issn.1001-909X.2017.03.010

PDF(1286 KB)

海洋学研究 ›› 2017, Vol. 35 ›› Issue (3) : 85-90. DOI: 10.3969/j.issn.1001-909X.2017.03.010

研究报道

仿刺参采苗板上细菌的分离与鉴定

卢晓平, 许艳蕊, 范秋苹, 王春蕾, 郝鲁江^*

作者信息 +

Isolation and identification of bacteria in Apostichopusjaponicus seedling in aquaculture environments

LU Xiao-ping, XU Yan-rui, FAN Qiu-ping, WANG Chun-lei, HAO Lu-jiang^*

Author information +

文章历史 +

摘要

为了解仿刺参采苗板时期细菌的多样性,对分离自仿刺参采苗板上的细菌进行了分离和鉴定。应用常规生理生化鉴定、16S rDNA序列分析和构建系统发生树对其进行分子鉴定。结果表明:共分离得到19株海洋细菌,鉴定为7个属,包括短芽孢杆菌属(Brevibacillus sp.)、假交替单胞菌属(Pseudoalteromonas sp.)、Cobetia属、肠杆菌属(Enterobacter sp.)、芽孢杆菌属(Bacillus sp.)、普罗威登斯菌属(Providencia sp.)和海洋螺菌属(Oceanospirillaceae sp.)。从海洋环境中分离筛选细菌对海洋资源的开发与利用具有重要意义,此外对仿刺参育苗时期进行细菌种类的鉴定有助于疾病的预防和治疗,对仿刺参的水产养殖具有一定的指导意义。

Abstract

To understand the diversity of bacteria during the seedling stage of Apostichopusjaponcicus, bacteria were isolated and identified. On the basis of the morphology, physiology and biochemistry, 16S rDNA sequence homology analysis, the 19 marine bacteria were identified to be Brevibacillus sp., Pseudoalteromonas sp., Cobetia sp., Enterobacter sp., Bacillus sp., Providenciasp., and Oceanospirillaceae sp.. Isolation and screening of bacteria from the marine environment is of great significance to the development and utilization of marine resources. In addition, the identification of the bacterial species in the period of seedling stage of Apostichopusjaponcicus is helpful to the prevention and treatment of disease, and it has a certain guiding significance for the aquaculture of Apostichopusjaponcicus.

导出引用

卢晓平, 许艳蕊, 范秋苹, 王春蕾, 郝鲁江. 仿刺参采苗板上细菌的分离与鉴定[J]. 海洋学研究. 2017, 35(3): 85-90 https://doi.org/10.3969/j.issn.1001-909X.2017.03.010

LU Xiao-ping, XU Yan-rui, FAN Qiu-ping, WANG Chun-lei, HAO Lu-jiang. Isolation and identification of bacteria in Apostichopusjaponicus seedling in aquaculture environments[J]. Journal of Marine Sciences. 2017, 35(3): 85-90 https://doi.org/10.3969/j.issn.1001-909X.2017.03.010

中图分类号： Q93-331

参考文献

[1] SUN Hai-hong, MAO Wen-jun, QIAN Ye-miao, et al. Advances on the characteristics and bioactivities of exopolysaccharides from marine microorganism[J].Marine Sciences,2011,35(11):134-138.
孙海红,毛文君,钱叶苗,等.海洋微生物活性胞外多糖的研究进展[J].海洋科学,2011,35(11):134-138.
[2] JIANG Jian, YANG Bao-ling, YUAN Qi, et al. Isolation and identification of marine bacteria with antimicrobial activity[J].Biotechnology,2004,14(6):28-30.
姜健,杨宝灵,元起,等.具有抑菌活性的海洋细菌的分离与鉴定[J].生物技术,2004,14(6):28-30.
[3] BERNAN V S, GREENSTEIN M, MAIESE W M. Marine microrgnisms as a source of new natural products[J]. Applied Microbial,1997,43(4):57-90.
[4] MATSUDA M, SHIGETA S, OKUTANI K. Antiviral activities of marine pseudomonas polysaccharides and their oversulfated derivative[J].Marine Biotechnology,1999,1(1):68-73.
[5] SUN Cheng, WANG Jian-wen, LEI Fang, et al. Free radical scavenging and antioxidant activities of EPS2,an exopolysaccharide produced by a marine filamentousfungus Keissleriella sp. YS 4108[J]. Life Science,2004,75(9):1 063-1 073.
[6] LI Jiang, CHEN Kao-shan, LI Ying-yu, et al. Phylogenetic analysis of antarctic bacterium Pseudoalteromonas sp. S-15-13 and the effect of its extracellular polysaccharide on proliferation of murine lymphocytes[J].Chinese Journal of Marine Drugs,2006,25(1):1-5.
李江,陈靠山,李莹玉,等.南极细菌Pseudoalteromonas sp.S-15-13的分子鉴定及其胞外多糖促进小鼠脾淋巴细胞转化的作用[J].中国海洋药物杂志,2006,25(1):1-5.
[7] DONG Xiu-zhu, CAI Miao-ying. Common bacterial system identification manual[M].Beijing:Science Press,2001:365-375.
东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:365-375.
[8] ZHOU De-qing.Microbiology experiment tutorial[M].2nd ed. Beijing: Higher Education Press,2006:367-385.
周德庆.微生物学实验教程[M].第2版.北京:高等教育出版社,2006:367-385.
[9] THOMPSON J D, GIBSON T J, PLEWNIAK F, et al. The Clustal-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research,1997,25(24):4 876-4 882.
[10] SAITOU N, NEI M. The neighbor-joining method: a new method for reconstructing phylogenetic trees[J]. Molecular Biology and Evolution,1987,4(4):406-425.
[11] DEVEREUX R, HE S H, DOYLE C L, et al. Diversity and origin of Desulfovibrio species: Phylogenetic definition of a family[J].J Bacteriol,1990,172(7):3 609-3 619.
[12] FRY N K, WARWICK S, SAUNDERS N A, et al. The use of 16S ribosomal RNA analyses to investigate the phylogeny of the family Legionellaceae[J].J Gen Microbiol,1991,137(5):1 215-1 222.
[13] DAS S, MANGWANI N. Ocean acidification and marine microorganisms: Responses and consequences[J].Oceanologia,2015,57(4):349-361.
[14] LI Wei, ZHENG Wei, ZHENG Tian-ling. Advances in research of marine microbial diversity and molecular ecology[J].Microbiology China,2013,40(4):655-668.
李炜,郑伟,郑天凌.海洋微生物多样性及其分子生态学研究进展[J].微生物学通报,2013,40(4):655-668.
[15] AZAM F, WORDEN A Z. Microbes, molecules, and marine ecosystems[J].Science,2004,303(5664):1 622-1 624.
[16] XUE Chao-bo, WANG Guo-liang, JIN Shan, et al.Advances in marine microbial diversity research[J].Advances in Marine Science,2004,22(3):377-384.
薛超波,王国良,金珊,等.海洋微生物多样性研究进展[J].海洋科学进展,2004,22(3):377-384.
[17] WANG Lin, YANG Qiu-ming, HAO Jian-dong, et al.Isolation and identification of bacteria from the environment of mass mortality during Haliotis diversicolor supertexta larvae stage[J].Journal of Oceanography in Taiwan Strait,2009,28(2):210-216.
汪琳,杨秋明,郝剑东,等.九孔鲍鲍苗脱板期养殖环境细菌分离及分子鉴定[J].台湾海峡,2009,28(2):210-216.
[18] XI Yu, ZHU Da-heng, LIU Hong-tao, et al.Advances on Pseudoalteromonas species and their extracellular bioactive compounds[J].Microbiology China,2005,32(3):108-112.
席宇,朱大恒,刘洪涛,等.假交替单胞菌及其胞外生物活性物质研究进展[J].微生物学通报,2005,32(3):108-112.
[19] POLI A, ANZELMO G, NICOLAUS B. Bacterial exopolysaccharides from extreme marine habitats: production, characterization and biological activities[J]. Mar Drugs,2010,8(6):1 779-1 802.
[20] NICHOLS C M, lARDIÈRE S G, BOWMAN J P, et al. Chemical characterization of exopolysaccharides from Antarctic marine bacteria[J]. Microb Ecol,2005,49(4):578-589.
[21] LI W W, ZHOU W Z, ZHANG Y Z, et al. Flocculation behavior and mechanism of an exopolysaccharide from the deep-sea psychrophilic bacterium Pseudoalteromonas sp. SM9913[J]. Bioresour Technol,2008,99(15):6 893-6 899.