浊度对活体叶绿素荧光检测的影响及修正方法

doi:10.3969/j.issn.1001-909X.2018.02.004

海洋学研究 ›› 2018, Vol. 36 ›› Issue (2): 27-35.DOI: 10.3969/j.issn.1001-909X.2018.02.004

浊度对活体叶绿素荧光检测的影响及修正方法

叶陈军^1,2, 潘建明^*1,2, 郑旻辉^1,2, 谢尚微^1,2, 章哲超^1,2

1.国家海洋局海洋生态系统与生物地球化学重点实验室,浙江杭州 310012;
2.国家海洋局第二海洋研究所,浙江杭州 310012

收稿日期:2017-12-21 修回日期:2018-03-27 出版日期:2018-06-15 发布日期:2022-11-21
通讯作者: *潘建明(1964-),男,研究员,主要从事海洋生物地球化学方面的研究。E-mail:jmpan@sio.org.cn
作者简介:叶陈军(1993-),男,浙江衢州市人,主要从事海洋生物地球化学方面的研究。E-mail:1546347078@qq.com
基金资助:
浙江省自然科学基金项目资助(LQ16D060006);中央级公益性科研院所基本科研业务费专项项目资助(JG1715,JT1502);国家海洋公益性行业科研专项项目资助(201305012)

The influence of turbidity on the measurement of in vivo chlorophyll fluorescence and its correction method

YE Chen-jun^1,2, PAN Jian-ming^*1,2, ZHENG Min-hui^1,2, XIE Shang-wei^1,2, ZHANG Zhe-chao^1,2

1. Laboratory of Marine Ecosystem and Biogeochemistry,SOA,Hangzhou 310012,China;
2. Second Institute of Oceanography,SOA,Hangzhou 310012,China

Received:2017-12-21 Revised:2018-03-27 Online:2018-06-15 Published:2022-11-21

摘要/Abstract

摘要： 本文研究了泥沙悬浮液浊度对叶绿素荧光计测定水样中活体叶绿素荧光的影响。结果表明,浊度产生的荧光效应和遮蔽效应对活体叶绿素荧光测定具有显著影响。在100 NTU浊度范围内,浊度会导致荧光效应使活体叶绿素荧光值测量结果随着浊度的增大而增大,并构成良好的线性关系;实际测定水体中硅藻的活体叶绿素荧光值时,泥沙悬浮液的浊度会导致遮蔽效应使活体叶绿素荧光值测量结果随着浊度的增大而减小,水体中硅藻质量浓度越高遮蔽效应越显著,在1 000 mV(约10 μg/L)硅藻质量浓度,100 NTU浊度条件下,浊度遮蔽效应可导致活体叶绿素荧光值偏低20%。根据近海泥沙实验数据,本文提出了一种活体叶绿素荧光检测的浊度影响修正方法,在100 NTU浊度内,修正后叶绿素荧光值偏差率都下降到±10%以内,校准后的荧光值与真实叶绿素荧光值较为接近。

关键词: 荧光计, 叶绿素荧光, 浊度, 泥沙, 修正方法

Abstract: The influence of sediment turbidity on chlorophyll sensor determination of in vivo chlorophyll fluorescence was studied. The results show that turbidity can cause fluorescence effect and light shielding effect on the determination of chlorophyll fluorescence. In the range of 100 NTU turbidity, the fluorescence value of chlorophyll shows a good linear relationship with the turbidity of sediment suspension, which increases with the increase of turbidity. When actually measuring the in vivo chlorophyll fluorescence of diatoms in water, turbidity of sediment suspension causes light shielding effect that decreases in vivo chlorophyll fluorescence as turbidity increases. The concentration of microalgae in the water is higher, the light shielding effect is stronger. In 1 000 mV (including 10 μg/L), the highest concentration of microalgae, sediment suspension can reduce the chlorophyll fluorescence value by 20% at 100 NTU turbidity. Based on the experimental data, a correction method of turbidity effect of in vivo chlorophyll fluorescence detection is proposed. Within 100 NTU turbidity, the corrected deviation of chlorophyll fluorescence values is reduced to less than ±10% and the corrected fluorescence value is close to the true chlorophyll fluorescence.

Key words: Fluorometer, chlorophyll fluorescence, turbidity, sediment, correction method

中图分类号:

P716⁺.6

叶陈军, 潘建明, 郑旻辉, 谢尚微, 章哲超. 浊度对活体叶绿素荧光检测的影响及修正方法[J]. 海洋学研究, 2018, 36(2): 27-35.

YE Chen-jun, PAN Jian-ming, ZHENG Min-hui, XIE Shang-wei, ZHANG Zhe-chao. The influence of turbidity on the measurement of in vivo chlorophyll fluorescence and its correction method[J]. Journal of Marine Sciences, 2018, 36(2): 27-35.

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浊度对活体叶绿素荧光检测的影响及修正方法

The influence of turbidity on the measurement of in vivo chlorophyll fluorescence and its correction method

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