不同模式高盐胁迫对浒苔光合生理及相关基因表达的影响

钟佳丽; 李亚鹤; 郑明山; 臧茹; 徐年军

doi:10.3969/j.issn.1001-909X.2019.02.008

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海洋学研究 ›› 2019, Vol. 37 ›› Issue (2) : 72-80. DOI: 10.3969/j.issn.1001-909X.2019.02.008

研究论文

不同模式高盐胁迫对浒苔光合生理及相关基因表达的影响

钟佳丽^1,2, 李亚鹤^1,2, 郑明山^1,2, 臧茹^1,2, 徐年军^*1,2

作者信息 +

Effects of different hypersalinity models on the photo-physiological performances and related gene expression in Ulva prolifera

ZHONG Jia-li^1,2, LI Ya-he^1,2, ZHENG Ming-shan^1,2, ZANG Ru^1,2, XU Nian-jun^*1,2

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文章历史 +

摘要

为探讨高盐及盐度变化对浒苔光合生理的影响以及浒苔在面临高盐胁迫时表现出的耐盐性,本文选取浒苔(Ulva prolifera)为供试材料,设置3个盐度变化处理组:对照组(CK)始终保持盐度25;处理组1(T1)经过盐度30适应3 d后增加到盐度35;处理组2(T2)长期处于盐度35,研究高盐及盐度变化对浒苔生长、光合色素含量、暗呼吸速率和光合放氧速率以及核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco)和钙调蛋白(CaM)的基因表达量水平的影响。结果表明:与对照组相比,高盐会抑制浒苔的相对生长速率和光合生理,T1、T2处理组均显著抑制浒苔的生长以及净光合作用速率,且T2处理组的抑制作用更强。高盐处理对浒苔光合色素的含量及其比值、暗呼吸速率的影响不显著,但随着藻体的成熟,浒苔光合色素的含量显著降低,并且暗呼吸速率显著增加。高盐也同时影响了rbcL和cam的基因表达水平,比较0、3和6 d三个取样点结果,T1盐度递增组的基因表达量高于T2长期高盐组,盐度逐步提高有利于增加浒苔对高盐度的适应能力。这一结果有助于解释浒苔在形成绿潮草垫以及退潮干出期间对盐度提高的适应。

Abstract

To evaluate the effects of hypersalinity and its fluctuation on the photo-physiological and the performances of marine “green tide” algae on salt tolerance, Ulva prolifera was selected as the experimental material. We set up three salinity conditions: salinity 25 as control (CK); treatment 1 (T1), salinity 30 for 3 d, then increased to salinity 35; treatment 2 (T2), salinity 35 for 6 d. The relative growth rate, the content of photosynthetic pigments, the photosynthetic and dark respiration rates and the gene expression levels of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) and calmodulin (CaM) were measured. The results showed that compared with control groups, hypersalinity significantly inhibited the relative growth rates and photo-physiological performances. Both T1, T2 treatments inhibited the relative growth rates and net photosynthesis rates of U. prolifera, and the inhibition effects of T2 was much stronger than that of the former one. The effect of hypersalinity stress on the dark respiration rate was not significant, which also had no significant effect on the content of photosynthetic pigments and their ratio of photosynthetic pigments. However, the dark respiration rate was significantly increased and photosynthetic pigments was significantly decreased along with the algae grow up. And the gene expression levels of rbcL and cam were also affected by hypersalinity. U. prolifera cultured in T1 treatment showed better resistance to hypersalinity than T2 treatment after 3 d and 6 d. It showed that U. prolifera could adapt to hypersalinity when the salinity was increased gradually. These results could explain the adaptation ability on hypersalinity stress of U. prolifera at the period of green tide formed and low tide.

导出引用

钟佳丽, 李亚鹤, 郑明山, 臧茹, 徐年军. 不同模式高盐胁迫对浒苔光合生理及相关基因表达的影响[J]. 海洋学研究. 2019, 37(2): 72-80 https://doi.org/10.3969/j.issn.1001-909X.2019.02.008

ZHONG Jia-li, LI Ya-he, ZHENG Ming-shan, ZANG Ru, XU Nian-jun. Effects of different hypersalinity models on the photo-physiological performances and related gene expression in Ulva prolifera[J]. Journal of Marine Sciences. 2019, 37(2): 72-80 https://doi.org/10.3969/j.issn.1001-909X.2019.02.008

中图分类号： Q945.78

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