利用线粒体COX和叶绿体rbcL基因片段对我国东南沿海坛紫菜(Pyropia haitanensis Chang et Zheng)9个野生群体和2个养殖群体(共125个个体)进行遗传多样性分析。比对分析后得到408 bp的COX基因片断和488 bp的rbcL基因片断。联合分析的基因片断长度为896 bp,其中T、C、A、G的含量分别为34.9%、14.6%、34.2%和16.3%。检测到变异位点128个,其中单碱基变异位点19个,简约信息位点109个。群体的平均核苷酸多样性为0.035 9±0.028 4,单倍型多样性为0.817 0±0.024 0。125个个体共定义了31个单倍型,其中Hap1、Hap10和Hap16是核心单倍型,分别占全部样本的32.8%、12.8%和24.8%。群体遗传距离和地理距离相关性检测显示二者呈线性相关(R2=0.002 2,p=0.735 4),即遗传距离随地理距离的增大而增大,但未形成地理隔离。根据单倍型构建的ML树显示,坛紫菜群体之间没有明显的地理谱系结构,表明各个群体之间存在基因交流。
Abstract
Pyropia haitanensis is one of the most important economic seaweeds for mariculture in China. In order to investigate genetic diversity of P. haitanensis, we collected 125 individuals from 11 populations along the southeast coast of China. We amplified 408 bp length of COX gene fragments and 488 bp length of rbcL gene fragments. The concatenated COX and rbcL gene fragments had a length of 896 bp, and the ratios of T, C, A, G were 34.9%, 14.6%, 34.2% and 16.3%, respectively. We detected 128 mutation sites, including 19 single-base mutation and 109 simple information sites. The mean nucleotide and haplotype diversity of 11 populations were 0.035 9±0.028 4 and 0.817 0±0.024 0, respectively. The concatenated COX and rbcL gene fragments defined 31 haplotypes from 125 individuals, and Hap1,Hap10 and Hap16 were the core haplotypes, accounting for 32.8%, 12.8% and 24.8% of all individuals, respectively. The result of distance analysis showed a linear correlation between population-pairwise genetic distances and geographic distances (R2=0.002 2, p=0.735 4). Genetic distance increased with the increased geographic distance, but geographic isolation had not yet been formed. The ML tree constructed from haplotypes showed no obvious geographical lineage structure in P. haitanensis, indicating potential gene exchange among populations.
关键词
坛紫菜 /
COX基因 /
rbcL基因 /
遗传多样性
Key words
Pyropia haitanensis /
COX gene /
rbcL gene /
genetic diversity
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基金
浙江省水产新品种选育重大科技专项(2016C02055-6);温州市农业新品种选育协作组项目(2019ZX001);温州市科技项目(S2020008);农业部藻类产业技术体系(CARS-50)
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