海洋学研究 ›› 2012, Vol. 30 ›› Issue (2): 81-85.

• 研究报道 • 上一篇    下一篇

美国红鱼肾肿大症的组织病理和超微病理分析

彭蟆, 朱文渊, 王国良*   

  1. 宁波大学生命科学与生物工程学院,浙江宁波 315211
  • 收稿日期:2011-05-26 修回日期:2011-07-11 出版日期:2012-06-15 发布日期:2023-04-24
  • 通讯作者: * 王国良,教授,E-mail: wangguoliang@nbu.edu.cn
  • 作者简介:彭頉(1987-),女,浙江平湖市人,硕士研究生,主要从事水产动物病害研究。E-mail.jxphpd@163.com
  • 基金资助:
    教育部长江学者与创新团队项目(IRTO734);宁波市自然科学基金资助项目(2009A610116)

Histopathological and ultrapathological study on kidneyintumesce of American red drum,sciaenops ocellatus

PENG Di, ZHU Wen-yuan, WANG Guo-liang*   

  1. Faculty of Life Science and Biotechnology of Ningbo University,Ningbo 315211,China
  • Received:2011-05-26 Revised:2011-07-11 Online:2012-06-15 Published:2023-04-24

摘要: 采用组织学方法,获得患肾肿大症美国红鱼的心﹑脾、肾﹑肝﹑肠和鳃6种组织,利用组织切片以及超薄切片电镜技术对患病组织的病理变化进行了研究分析,探讨该病的致病机制。患病红鱼解剖后主要症状为:体表溃烂,部分病鱼鳍条上有肿块;病鱼肾脏显著肿大,体积甚至达到正常肾脏的10倍|肝脏﹑脾脏及胆囊等也有肿大现象;有些病鱼脏器上有白色结节。组织病理显示:美国红鱼肾肿大症的主要表现为肾脏有典型的肉芽肿,大量炎性细胞浸润,肾小管管壁破裂、坏死;肝脏中肝实质有充血现象,实质细胞呈空泡变性;脾脏充血,现大量病变的红细胞,有脂肪样变。超微病理显示:肾近曲小管上皮细胞凋亡现象严重,肾小管内刷状缘排列稀疏|肝细胞坏死严重,肝细胞界限模糊不清;脾脏组织脾窦扩张,有大量凋亡红细胞和淋巴细胞。实验结果表明:美国红鱼肾肿大症的组织细胞结构发生明显的病理变化,肾脏和肝脏等主要组织器官受到严重损伤病变,使鱼体不能进行正常的生命活动,最后导致死亡。

关键词: 美国红鱼, 肾肿大症, 组织病理, 超微病理

Abstract: The heart,spleen,kidney,liver,intestine and gill from Sciaenops ocellatus infected with kidneyintumesce were pathologically analyzed by tissue slice and ultramicrocut electron microscopy techniques,so asto study the pathological changes of the tissues and the pathogenic mechanism of this disease.The anatomicalsymptoms of the diseased Sciaenops ocellatus are body surface ulceration,some lumps on the pterygiophore ofsome diseased fish,distinctly enlarged kidney (some 10 times as large as the normal one),enlarged liver,spleen and gallbladder, and white nodules on the viscera of some diseased fish.Histopathology shows that themain manifestations were typical granuloma,infiltration of large amount of inflammatory cells,rupture andnecrosis of the tubular wall of the renal tubule,parenchyma hyperemia and vacuolar degeneration of theparenchymal cells in the liver,and abundant diseased erythrocyte appeared in the spleen with hyperemia andfatty degeneration. Ultrastructure pathology shows serious apoptosis of the epithelial cells of the kidneyproximal tubule,sparse and ruptured brush border in the renal tubule,serious necrosis and blurredboundaries of the liver sell,and expansion of the splenic sinus of the spleen tissue,as well as abundantapoptosis cells of erythrocyte and lymphocytes. The results indicate that pathological changes widely occur intissue cells of the kidney intumesce Sciaenops ocellatus and cause serious damage to the body organs,whichmakes the infected fish lose normal physiological metabolism and died at last.

Key words: Sciaenops ocellatus, kidney intumesce, histopathology, ultrastructure

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