海洋学研究 ›› 2012, Vol. 30 ›› Issue (2): 86-92.

• 研究报道 • 上一篇    

10种蟹类18S rDNA 和COⅠ基因序列分析及其分子系统发育研究

邢炳鹏, 林荣澄*, 徐宪忠, 牛文涛   

  1. 国家海洋局第三海洋研究所,福建厦门 361005
  • 收稿日期:2010-12-10 修回日期:2011-09-09 出版日期:2012-06-15 发布日期:2023-04-24
  • 通讯作者: * 林荣澄,研究员,E-mail: rclin@public.xm.fj.cn
  • 作者简介:邢炳鹏(1983-),男,山东冠县人,硕士,助理研究员,主要从事海洋生物技术研究。E-mail:erpeng8338@163.com
  • 基金资助:
    中国大洋协会国际海底区域研究开发“十一五”资助项目(DYXM-115-02-4-05)

18S rDNA and Co I gene sequence analysis of 10 crabs and study of their molecular phylogeny

XING Bing-peng, LIN Rong-cheng*, XU Xian-zhong, NIU Wen-tao   

  1. Third Institute of Oceanography,SOA,Xianmen 361005,China
  • Received:2010-12-10 Revised:2011-09-09 Online:2012-06-15 Published:2023-04-24

摘要: 应用分子系统发育学的方法,以蟹类18S rDNA和线粒体细胞色素C氧化酶亚单位Ⅰ(COⅠ)基因序列片段为分子标记,结合形态学特征对10种蟹类的分类地位进行探讨。实验共获得10 条18S rDNA序列,长度为1780~1787 bp,其中A、T, G,C平均含量分别为23.72%, 24.58%,24.52%和27.17%;通过序列对比,发现18S rDNA序列相对保守,只含有1个从88 bp 到130 bp 约 50 bp的高可变区;物种间碱基距离比较小,从0.001到0.017。18S rDNA系统发育树为方蟹总科、沙蟹总科和梭子蟹总科起源于同-海洋蟹类祖先提供了分于生物q证惦,开上火亚N内尔量分别为26.88%.弓蟹科。获得的5条CO Ⅰ基因序列,长度均为709 bp,A、T,G、C平均含量分别为26.88%,37.62%,17.50%和18.00%;COⅠ基因片段变异性高,物种间碱基距离从0.016到0.138。COⅠ基因系统发育树真实地反映了住属各物种之间的进化关系,和传统分类非常吻合,为形态特征相似的姆类鉴定提供了-种快速准确的方法。

关键词: 蟹类, 18S rDNA, COl, 分子系统发育

Abstract: Using molecular phylogenetic methodology with gene sequence fragments of 18S rDNA and subunitl of C enzyme oxide of mitochondrial dell pigment(CO I) as the molecular mark and combined withmorphological character,evolutionary history of selected brachyuran crabs were discussed. Totally 10 18SrDNA sequnces and 5 CO I gene fragments were obtained by Polymerase Chain Reaction (PCR),andsummited to the Genebank. The length of 18S rDNA sequences from 10 crabs,ranges from 1 780 to l787 bp,in which the shortest one is Hemigrapsus sinensis, the longest one is Parthenope validus. In the 18S rDNAsequnces, the average content of base A,T,G and C is 23.72%,24.58%,24.52%,and 27.17% separately.Sequence alignment shows that 18S rDNA sequences are relatively conservative,containing only onehypervariable region of about 50 bp between 88 bp and 130 bp. The base distance of the species is very small,from 0.001 to 0.017.The phylogenetic trees of 18S rDNA give molecular biological proof for the same originof Grapsioidea,Ocypodioidea and Portunoidea, and also support moving the Helice crab from Sesarminae toVaruninae.The length of mitochondrial DNA sequence from 5 charybdis crabs is 709 bp,and the averagecontent of the base A,T,G and C is 26.88%,37.62%,17.50% and 18.00%separately.The fragment ofco I gene has high species variability,more suitable for the study of the interspecific evolutionary history.The base distance of the charybdis is long,from 0.016 to 0.138.The phylogenetic trees of CO I gene truereflect the evolutionary relations of different charybdis species and are concordant with traditional taxology.This provides a molecular method for the identification of charybdis species of similar morphologicalcharact ers.

Key words: crab, 18S rDNA, COl, phylogenesis

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