CO2和DIP对浒苔幼苗生长及光合特性的影响

doi:10.3969/j.issn.1001-909X.2023.02.010

海洋学研究 ›› 2023, Vol. 41 ›› Issue (2): 114-122.DOI: 10.3969/j.issn.1001-909X.2023.02.010

CO₂和DIP对浒苔幼苗生长及光合特性的影响

王津果¹^,²^,³(), 盛杨杰¹^,²^,³^,⁴, 王续昆¹^,²^,³, 倪嘉璇¹^,²^,³, 武卉¹^,²^,³, 刘卫国⁵, 周伟¹^,²^,³^,⁵^,^*()

1.江苏海洋大学,江苏省海洋生物资源与环境重点实验室,江苏连云港 222005
2.自然资源部滨海盐沼湿地生态与资源重点实验室,江苏连云港 222005
3.江苏海洋大学,江苏省海洋生物产业技术协同创新中心,江苏连云港 222005
4.汕头大学理学院,广东汕头515000
5.乳山市牡蛎协会,山东威海 264500

收稿日期:2022-04-02 修回日期:2022-06-29 出版日期:2023-06-15 发布日期:2023-07-27
通讯作者: *周伟(1985—),男,博士,高级工程师,主要从事水产育苗和养殖等研究,E-mail:wzhou@jou.edu.cn。
作者简介:王津果(1989—),女,河南省许昌市人,博士,主要从事海洋生物遗传育种研究,E-mail:2020000035@jou.edu.cn。
基金资助:
江苏省自然资源发展专项项目(JSZRHYKJ202206);江苏省自然资源发展专项项目(JSZRHYKJ202107);江苏省高等学校大学生创业训练计划(2021120148);江苏海洋大学人才引进科研基金项目(KQ19068);连云港市“花果山英才计划”(KK21054);江苏省优势学科建设工程资助项目

Effects of CO₂ and dissolved inorganic phosphate on the growth and photosynthetic performance of Ulva prolifera seedlings

WANG Jinguo¹^,²^,³(), SHENG Yangjie¹^,²^,³^,⁴, WANG Xukun¹^,²^,³, NI Jiaxuan¹^,²^,³, WU Hui¹^,²^,³, LIU Weiguo⁵, ZHOU Wei¹^,²^,³^,⁵^,^*()

1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China
2. Key Laboratory of Coastal Salt Marsh Ecosystems and Resources, MNR, Lianyungang 222005, China
3. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005,China
4. College of Science, Shantou University, Shantou 515000, China
5. Rushan Oyster Association, Weihai 264500, China

Received:2022-04-02 Revised:2022-06-29 Online:2023-06-15 Published:2023-07-27

摘要/Abstract

摘要：

绿藻微观繁殖体具有快速生长和繁殖的特性,是绿潮暴发重要的种子来源。以浒苔(Ulva prolifera)微观繁殖体发育而来的幼苗为实验材料,研究不同CO₂水平(400 μatm和1 000 μatm)和无机磷酸盐(dissolved inorganic phoshphate, DIP)浓度(0.32 μmol·L^-1、3.62 μmol·L^-1、36.2 μmol·L^-1)下单因子及双因子交互作用对其生长和光合生理指标的影响。相对生长速率(RGR)、净光合速率(P_n)和呼吸速率(R_d)等生长指标显示:CO₂对RGR和P_n有显著影响(p<0.05);DIP对RGR、P_n和R_d均有显著影响(p<0.05),随着DIP增加,RGR和P_n升高,R_d降低;双因子交互作用对3个指标影响均不显著(p>0.05)。最大相对光合电子传递速率(rETR_max)、饱和光强(E_k)、有效光合量子产率[Y(II)]、光能利用效率(α)等荧光参数显示:CO₂对rETR_max、E_k影响显著(p<0.05); DIP浓度升高使Y(II)、rETR_max、α和E_k显著提高(p<0.05);双因子交互作用对rETR_max和Y(II)影响显著(p<0.05),对α和E_k影响不显著(p>0.05)。Chl a、Chl b和类胡萝卜素等色素指标显示:CO₂、DIP以及交互作用对3个指标影响均显著(p<0.05),高CO₂水平明显抑制色素的合成,并且随着DIP浓度升高,抑制程度加剧;3个指标与DIP均呈正相关。研究表明,高CO₂水平和高DIP浓度会明显促进浒苔幼苗的生长,为绿潮的暴发提供了有利条件。

关键词: 浒苔, 微观繁殖体, CO₂, 无机磷酸盐, 生长

Abstract:

Micropropagules with rapid growth and reproduction are an important seed sources for the early outbreak of the green tide. In this study, the micropropagule seedlings of Ulva prolifera were collected as experimental materials, and two CO₂ levels (LC, 400 μatm; HC, 1 000 μatm) and three dissolved inorganic phoshphate (DIP) concentrations (LP, 0.32 μmol/L; MP, 3.62 μmol/L; HP, 36.2 μmol/L) were set to analyze the effects of CO₂, DIP and their interaction on its growth and photophysiological performance. The results of the growth indexes including relative growth rate (RGR), net photosynthetic rate (P_n) and respiration rate (R_d) showed that CO₂ significantly affected on the RGR and P_n (p<0.05). DIP significantly affected on the RGR, P_n and R_d (p<0.05). Specifically, RGR and P_n were significantly positively correlated with DIP concentration (p<0.05), while R_d was significantly negatively correlated with DIP concentration (p<0.05). However, their interaction had no significant effect on RGR, P_n and R_d (p>0.05). The results of fluorescence parameters including effective quantum yield [Y(II)], maximum relative electron transfer rate (rETR_max), saturation light intensity (E_k) and light utilization efficiency indicated (α) reveal that CO₂ significantly affected on the rETR_max and E_k (p<0.05), but had no significant effect on the Y(II) and α (p>0.05). DIP significantly affected all the fluorescence parameters, which were significantly promoted by the DIP concentration increase (p<0.05). And their interaction had significant effect on the rETR_max and Y(II) (p<0.05), but had no significant effect on the E_k and α (p>0.05). Pigments of Chl a, Chl b and carotenoid results showed that CO₂, DIP and their interaction significantly affected the pigments contents (p<0.05). Evelated-CO₂ significantly inhibited pigment synthesis (p<0.05), and the inhibitory effect was enhanced with the increasing of DIP concentration. The pigments contents were significantly positively correlated with DIP concentration (p<0.05). Our findings suggested that evelated-CO₂ and higher DIP concentration obviously promoted the growth of U.prolifera seedlings, which was favorable for the green tide outbreak.

Key words: Ulva prolifera, micropropagules, CO₂, dissolved inorganic phoshphate, growth

中图分类号:

S917.3

王津果, 盛杨杰, 王续昆, 倪嘉璇, 武卉, 刘卫国, 周伟. CO₂和DIP对浒苔幼苗生长及光合特性的影响[J]. 海洋学研究, 2023, 41(2): 114-122.

WANG Jinguo, SHENG Yangjie, WANG Xukun, NI Jiaxuan, WU Hui, LIU Weiguo, ZHOU Wei. Effects of CO₂ and dissolved inorganic phosphate on the growth and photosynthetic performance of Ulva prolifera seedlings[J]. Journal of Marine Sciences, 2023, 41(2): 114-122.

图/表 7

表1 不同处理组的海水碳酸盐系统参数

Tab.1 Parameters of seawater carbonate system under different treatments

处理组	pH	pCO₂/μatm	DIC/(μmol·kg^-1)	HC $O 3 -$ /(μmol·kg^-1)	C $O 3 2 -$ /(μmol·kg^-1)	CO₂/(μmol·kg^-1)	TA/(μmol·kg^-1)
LCLP	8.20±0.01^a	382.36±13.07^a	2 002.85±19.95^a	1 817.53±21.22^a	172.70±2.17^a	12.26±0.43^a	2 251.60±16.65^a
LCMP	8.18±0.02^a	408.82±24.69^a	2 036.32±33.82^a	1 854.54±36.41^a	168.29±3.41^a	13.49±0.81^a	2 276.87±27.12^a
LCHP	8.19±0.02^a	395.95±20.66^a	2 022.36±37.76^a	1 838.52±36.85^a	170.77±5.93^a	13.07±0.68^a	2 267.25±38.27^a
HCLP	7.85±0.00^b	931.28±15.51^b	2 092.77±34.85^b	1 978.08±32.94^b	83.96±1.40^b	30.73±0.51^b	2 194.97±35.74^a
HCMP	7.86±0.01^b	908.84±38.05^b	2 106.87±46.75^ab	1 989.78±44.75^b	87.09±2.30^b	30.00±1.26^b	2 214.06±46.52^a
HCHP	7.85±0.03^b	940.03±44.52^b	2 111.23±37.31^b	1 995.36±32.66^b	84.84±6.29^b	31.02±1.47^b	2 214.06±46.52^a

表1 不同处理组的海水碳酸盐系统参数

Tab.1 Parameters of seawater carbonate system under different treatments

处理组	pH	pCO₂/μatm	DIC/(μmol·kg^-1)	HC $O 3 -$ /(μmol·kg^-1)	C $O 3 2 -$ /(μmol·kg^-1)	CO₂/(μmol·kg^-1)	TA/(μmol·kg^-1)
LCLP	8.20±0.01^a	382.36±13.07^a	2 002.85±19.95^a	1 817.53±21.22^a	172.70±2.17^a	12.26±0.43^a	2 251.60±16.65^a
LCMP	8.18±0.02^a	408.82±24.69^a	2 036.32±33.82^a	1 854.54±36.41^a	168.29±3.41^a	13.49±0.81^a	2 276.87±27.12^a
LCHP	8.19±0.02^a	395.95±20.66^a	2 022.36±37.76^a	1 838.52±36.85^a	170.77±5.93^a	13.07±0.68^a	2 267.25±38.27^a
HCLP	7.85±0.00^b	931.28±15.51^b	2 092.77±34.85^b	1 978.08±32.94^b	83.96±1.40^b	30.73±0.51^b	2 194.97±35.74^a
HCMP	7.86±0.01^b	908.84±38.05^b	2 106.87±46.75^ab	1 989.78±44.75^b	87.09±2.30^b	30.00±1.26^b	2 214.06±46.52^a
HCHP	7.85±0.03^b	940.03±44.52^b	2 111.23±37.31^b	1 995.36±32.66^b	84.84±6.29^b	31.02±1.47^b	2 214.06±46.52^a

图1 不同处理下浒苔幼苗的相对生长速率 (不同小写字母表示在LC条件下不同处理组间差异显著,p<0.05;不同大写字母表示在HC条件下不同处理组间差异显著,p<0.05;*表示同一DIP浓度下不同CO2处理组间差异显著,p<0.05。)

Fig.1 Relative growth rate of U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05), and different capital letters represent significant differences among different treatments under HC, p<0.05; Asterisk represents significant differences between LC and HC within a DIP treatment,p<0.05.)

图2 不同处理下浒苔幼苗的有效光合量子产率 (不同小写字母表示在LC条件下不同处理组间差异显著, p<0.05;不同大写字母表示在HC条件下不同处理组间差异显著, p<0.05。)

Fig.2 Effective quantum yield of U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05), and different capital letters represent significant differences among different treatments under HC, p<0.05.)

图3 不同PAR下浒苔幼苗的相对光合电子传递速率

Fig.3 Relative electron transfer rate of U.prolifera seedlings under different PAR

表2 不同处理下浒苔幼苗的最大相对光合电子传递速率(rETRmax)、光能利用效率(α)与饱和光强(Ek)

Tab.2 The maximum rETR (rETRmax), light utilization efficiency (α) and saturation light intensity (Ek) of U.prolifera seedlings under different treatments

处理组	最大相对光合电子传递速率 (rETR_max)/(μmol·m^-2·s^-1)	光能利用效率(α)	饱和光强(E_k)/ (μmol·m^-2·s^-1)
LCLP	61.89±3.12^a	0.29±0.02^a	211.38±6.35^a
LCMP	92.63±1.93^b	0.31±0.02^ab	302.23±23.16^b
LCHP	105.88±2.93^c	0.33±0.02^b	317.11±13.97^b
HCLP	48.39±1.28^A*	0.32±0.02^A	153.20±11.79^A*
HCMP	92.63±7.87^B	0.34±0.01^A	274.33±15.32^B
HCHP	104.79±6.13^B	0.35±0.03^A	303.19±38.09^B

图4 不同处理下浒苔幼苗的净光合速率(a)和呼吸速率(b) (不同小写字母表示在LC条件下不同处理组间差异显著,p<0.05;不同大写字母表示在HC条件下不同处理组间差异显著,p<0.05;*表示同一DIP浓度下不同CO2处理组间差异显著,p<0.05。)

Fig.4 Net photosynthetic rate (a) and respiration rate (b) of U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05, and different capital letters represent significant differences among different treatments under HC, p<0.05; Asterisk represents significant differences between LC and HC within a DIP treatment, p<0.05.)

图5 不同处理下浒苔幼苗的叶绿素a(a)、叶绿素b(b)与类胡萝卜素(c)含量 (不同小写字母表示在LC条件下不同处理组间差异显著,p<0.05;不同大写字母表示在HC条件下不同处理组间差异显著,p<0.05;*表示同一DIP浓度下不同CO2处理组间差异显著,p<0.05。)

Fig.5 The contents of chlorophyll a (a), chlorophyll b (b) and carotenoids (c) contents in U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05, and different capital letters represent significant differences among different treatments under HC, p<0.05; Asterisk represents significant differences between LC and HC within a DIP treatment, p<0.05.)

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CO₂和DIP对浒苔幼苗生长及光合特性的影响

Effects of CO₂ and dissolved inorganic phosphate on the growth and photosynthetic performance of Ulva prolifera seedlings

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[1]	于雷, 李三忠, 索艳慧, 王秀娟. 海岸海洋碳循环过程与CO₂负排放[J]. 海洋学研究, 2023, 41(1): 14-25.
[2]	刘婷宇, 白雁, 朱伯仲, 李腾, 龚芳. 高空间分辨率海表CO₂分压的卫星遥感反演算法:机器学习在秋季象山港的应用[J]. 海洋学研究, 2023, 41(1): 82-95.
[3]	苗燕熠, 王斌, 李德望, 金海燕, 江志兵, 马晓, 于培松, 陈建芳, 王俊洋. 大风事件对长江口及邻近海域海-气CO₂通量的影响[J]. 海洋学研究, 2020, 38(1): 42-49.
[4]	钟佳丽, 李亚鹤, 郑明山, 臧茹, 徐年军. 不同模式高盐胁迫对浒苔光合生理及相关基因表达的影响[J]. 海洋学研究, 2019, 37(2): 72-80.
[5]	赖雯奕, 娄亚敏, 徐继林, 周成旭, 严小军. 多胺对3株硅藻生长的影响[J]. 海洋学研究, 2018, 36(3): 96-100.
[6]	吕航宇, 白雁, 李骞, 江洪. 夏季珠江口海域海水CO₂分压的卫星遥感反演[J]. 海洋学研究, 2018, 36(2): 1-11.
[7]	黄世玉, 张丽莉. 鲍及牡蛎内脏可溶物培养球等鞭金藻的效果[J]. 海洋学研究, 2015, 33(4): 70-76.
[8]	李熠, 何海伦, 陈大可. 基于海水环境和气象参数经验公式估算的东海海-气CO₂通量[J]. 海洋学研究, 2012, 30(3): 30-40.