海洋赤杆菌科菌株的类胡萝卜素合成基因分析

doi:10.3969/j.issn.1001-909X.2024.01.009

摘要/Abstract

摘要：

赤杆菌科(Erythrobacteraceae)菌株广泛分布于海洋环境,可合成类胡萝卜素等多种色素。类胡萝卜素具有光保护和抗氧化能力,对于赤杆菌科菌株适应海洋生态系统具有重要作用。本研究收集了107个海洋来源的赤杆菌科细菌基因组,分析了类胡萝卜素合成途径中关键酶的编码基因crtEBIGYZW在基因组中的分布特征,构建了基于氨基酸序列的不同编码基因系统发育树,阐明了深海和浅海来源的赤杆菌科菌株中类胡萝卜素合成基因的存在和分布规律,探究了不同的类胡萝卜素合成基因在近海与深海来源的赤杆菌科菌株进化过程中的遗传模式。研究结果显示,海洋来源的赤杆菌科菌株均含有crtEIGZ基因,约98.1%的菌株含有crtBY基因,约43.9%的菌株中存在crtW基因,其类胡萝卜素合成基因在菌株间存在分布差异,但在深海与浅海来源上并未显示特异性。此外,通过比较不同编码基因的系统发育树拓扑结构发现,赤杆菌科菌株的类胡萝卜素合成基因crtBYZW与系统发育密切相关,而crtEIG基因多通过水平基因转移获得,这有助于更好地评估类胡萝卜素合成基因家族在赤杆菌科中的进化,也为其他海洋细菌中的类胡萝卜素合成途径及基因研究提供科学依据,从而有助于开发海洋来源的产类胡萝卜素菌株。

关键词: 赤杆菌科, 海洋, 类胡萝卜素合成基因, 系统发育, 进化

Abstract:

Erythrobacteraceae is widely distributed in marine environments and can synthesize various pigments such as carotenoids. Carotenoids have photoprotective and antioxidant abilities, and they can play a crucial role in the adaptation of Erythrobacteraceae to marine ecosystems. In this study, we obtained the genomes of 107 marine Erythrobacteriaceae strains, analyzed the distribution characteristics of carotenoid biosynthesis genes including crtEBIGYZW in their genomes, constructed phylogenetic trees based on the amino acid sequences of the carotenoid biosynthesis genes, and elucidated the presence and distribution of carotenoid biosynthesis genes in Erythrobacteriaceae from deep-sea and shallow-sea sources. The results suggested that all of marine Erythrobacteriaceae strains contained the crtEIGZ genes, 98.1% of strains contained the crtBY genes, and 43.9% of them contained the crtW gene, which differed among strains but no any specificities found among the deep-sea or shallow-sea sources. Additionally, in the comparison of the phylogenetic topology, it was found that the crtBYZW genes were vertically transferred from their ancestor, while the crtEIG genes were mainly horizontal gene transferred. Our study aids in the understanding of the carotenoid biosynthesis gene evolution in the family Erythrobacteriaceae and also serves as a scientific basis for the study of carotenoid synthesis pathways and genes in other marine bacteria, enables a thorough understanding of marine carotenoid-synthesizing strains.

Key words: Erythrobacteriaceae, marine, carotenoid biosynthesis gene, phylogeny, evolution

中图分类号:

Q939

岳小岚, 徐林, 符格意, 许学伟. 海洋赤杆菌科菌株的类胡萝卜素合成基因分析[J]. 海洋学研究, 2024, 42(1): 91-105.

YUE Xiaolan, XU Lin, FU Geyi, XU Xuewei. Investigation of carotenoid biosynthesis genes in marine Erythrobacteraceae strains[J]. Journal of Marine Sciences, 2024, 42(1): 91-105.

图/表 6

参考文献 38

[1]	吴月红, 许学伟. 赤杆菌科微生物分类研究进展[J]. 微生物学通报, 2016, 43(5):1082-1094.
	WU Y H, XU X W. Advances in the taxonomy of Erythrobacteraceae[J]. Microbiology China, 2016, 43(5): 1082-1094.
[2]	XU L, SUN C, FANG C, et al. Genomic-based taxonomic classification of the family Erythrobacteraceae[J]. International Journal of Systematic and Evolutionary Microbiology, 2020, 70(8): 4470-4495. DOI PMID
[3]	TONON L A C, MOREIRA A P B, THOMPSON F. The family Erythrobacteraceae[M]//The Prokaryotes. Berlin, Heidelberg: Springer, 2014: 213-235.
[4]	LEE K B, LIU C T, ANZAI Y, et al. The hierarchical system of the ‘Alphaproteobacteria’: description of Hypho-monadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.[J]. International Journal of Systematic and Evolutionary Microbiology, 2005, 55(5): 1907-1919.
[5]	SIDDARAMAPPA S, VISWANATHAN V, THIYAGARAJAN S, et al. Genomewide characterisation of the genetic diversity of carotenogenesis in bacteria of the order Sphingomonadales[J]. Microbial Genomics, 2018, 4(4): e000172.
[6]	GAMMONE M A, RICCIONI G, D’ORAZIO N. Marine carotenoids against oxidative stress: Effects on human health[J]. Marine Drugs, 2015, 13(10): 6226-6246. DOI PMID
[7]	MATSUMOTO M, IWAMA D, ARAKAKI A, et al. Altererythrobacter ishigakiensis sp. nov., an astaxanthin-producing bacterium isolated from a marine sediment[J]. International Journal of Systematic and Evolutionary Microbiology, 2011, 61(12): 2956-2961.
[8]	NIERO H, DA SILVA M A C, DE FELICIO R, et al. Carotenoids produced by the deep-sea bacterium Erythrobacter citreus LAMA 915: Detection and proposal of their biosynthetic pathway[J]. Folia Microbiologica, 2021, 66(3): 441-456.
[9]	DAS A, YOON S H, LEE S H, et al. An update on microbial carotenoid production: Application of recent metabolic engineering tools[J]. Applied Microbiology and Biotechnology, 2007, 77(3): 505-512. DOI PMID
[10]	BERMAN J, ZORRILLA-LÓPEZ U, FARRÉ G, et al. Nutritionally important carotenoids as consumer products[J]. Phytochemistry Reviews, 2015, 14(5): 727-743.
[11]	ROHMER M, KNANI M, SIMONIN P, et al. Isoprenoid biosynthesis in bacteria: A novel pathway for the early steps leading to isopentenyl diphosphate[J]. Biochemical Journal, 1993, 295(2): 517-524.
[12]	YE R W, STEAD K J, YAO H, et al. Mutational and functional analysis of the β-carotene ketolase involved in the production of canthaxanthin and astaxanthin[J]. Applied and Environmental Microbiology, 2006, 72(9): 5829-5837.
[13]	JAIN C, RODRIGUEZ-R L M, PHILLIPPY A M, et al. High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries[J]. Nature Communications, 2018, 9(1): 5114. DOI PMID
[14]	SEEMANN T. Prokka: Rapid prokaryotic genome annotation[J]. Bioinformatics, 2014, 30(14): 2068-2069. DOI PMID
[15]	XU L, WU Y H, ZHOU P, et al. Investigation of the thermophilic mechanism in the genus Porphyrobacter by comparative genomic analysis[J]. BMC Genomics, 2018, 19(1): 385.
[16]	LECHNER M, FINDEISS S, STEINER L, et al. Proteinortho: Detection of (Co-) orthologs in large-scale analysis[J]. BMC Bioinformatics, 2011, 12(1): 124.
[17]	KATOH K, STANDLEY D M. MAFFT multiple sequence alignment software version 7: Improvements in performance and usability[J]. Molecular Biology and Evolution, 2013, 30(4): 772-780. DOI PMID
[18]	CAPELLA-GUTIÉRREZ S, SILLA-MARTÍNEZ J M, GAB-ALDÓN T. trimAl: A tool for automated alignment trimming in large-scale phylogenetic analyses[J]. Bioinformatics, 2009, 25(15): 1972-1973.
[19]	NGUYEN L T, SCHMIDT H A, VON HAESELER A, et al. IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies[J]. Molecular Biology and Evolution, 2015, 32(1): 268-274.
[20]	AVALOS M, GARBEVA P, VADER L, et al. Biosynthesis, evolution and ecology of microbial terpenoids[J]. Natural Product Reports, 2022, 39(2): 249-272.
[21]	RANAWAT P, RAWAT S. Stress response physiology of thermophiles[J]. Archives of Microbiology, 2017, 199(3): 391-414. DOI PMID
[22]	ZUÑIGA C, ZARAMELA L, ZENGLER K. Elucidation of complexity and prediction of interactions in microbial communities[J]. Microbial Biotechnology, 2017, 10(6): 1500-1522. DOI PMID
[23]	RABBANI S, BEYER P, LINTIG J V, et al. Induced β-carotene synthesis driven by triacylglycerol deposition in the unicellular alga Dunaliella bardawil[J]. Plant Physiology, 1998, 116(4): 1239-1248.
[24]	LAMERS P P, VAN DE LAAK C C W, KAASENBROOD P S, et al. Carotenoid and fatty acid metabolism in light-stressed Dunaliella salina[J]. Biotechnology and Bioengi-neering, 2010, 106(4): 638-648.
[25]	LAMERS P P, JANSSEN M, DE VOS R C H, et al. Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga[J]. Journal of Biotechnology, 2012, 162(1): 21-27.
[26]	WANI A K, AKHTAR N, SHER F, et al. Microbial adaptation to different environmental conditions: Molecular perspective of evolved genetic and cellular systems[J]. Archives of Microbiology, 2022, 204(2): 144. DOI PMID
[27]	KLASSEN J L. Phylogenetic and evolutionary patterns in microbial carotenoid biosynthesis are revealed by comparative genomics[J]. PLoS One, 2010, 5(6): e11257.
[28]	SANDMANN G. Diversity and origin of carotenoid biosynthesis: Its history of coevolution towards plant photosynthesis[J]. New Phytologist, 2021, 232(2): 479-493.
[29]	SANTANA-MOLINA C, HENRIQUES V, HORNERO-MÉNDEZ D, et al. The squalene route to C30 carotenoid biosynthesis and the origins of carotenoid biosynthetic pathways[J]. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(52): e2210081119.
[30]	IGARASHI N, HARADA J, NAGASHIMA S, et al. Horizontal transfer of the photosynthesis gene cluster and operon rearrangement in purple bacteria[J]. Journal of Molecular Evolution, 2001, 52(4): 333-341. PMID
[31]	ARNOLD B J, HUANG I T, HANAGE W P. Horizontal gene transfer and adaptive evolution in bacteria[J]. Nature Reviews Microbiology, 2022, 20(4): 206-218.
[32]	BERTHON J Y, NACHAT-KAPPES R, BEY M, et al. Marine algae as attractive source to skin care[J]. Free Radical Research, 2017, 51(6): 555-567.
[33]	SHINDO K, MISAWA N. New and rare carotenoids isolated from marine bacteria and their antioxidant activities[J]. Marine Drugs, 2014, 12(3): 1690-1698. DOI PMID
[34]	SUBHASH Y, TUSHAR L, SASIKALA C, et al. Erythro-bacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern[J]. Interna-tional Journal of Systematic and Evolutionary Microbiology, 2013, 63(12): 4524-4532.
[35]	TAREEN S, RISDIAN C, MÜSKEN M, et al. Qipengyuania pacifica sp. nov., a novel carotenoid-producing marine bacterium of the family Erythrobacteraceae, isolated from sponge (Demospongiae), and antimicrobial potential of its crude extract[J]. Diversity, 2022, 14(4): 295.
[36]	YOON J, LEE E Y, NAM S J. Erythrobacter rubeus sp. nov., a carotenoid-producing alphaproteobacterium isolated from coastal seawater[J]. Archives of Microbiology, 2022, 204(2): 125.
[37]	GALASSO C, CORINALDESI C, SANSONE C. Carotenoids from marine organisms: Biological functions and industrial applications[J]. Antioxidants, 2017, 6(4): 96.
[38]	RAM S, MITRA M, SHAH F, et al. Bacteria as an alternate biofactory for carotenoid production: A review of its applications, opportunities and challenges[J]. Journal of Functional Foods, 2020, 67: 103867.

序号	GenBank登录号	菌株	基因组大小/bp	基因组GC含量/%	Contig数量/个	来源
1	GCA_001626395.1	Erythrobacter sp. REDSEA-S40_B1	2 688 120	63.7	7	浅海海水
2	GCA_001626415.1	Erythrobacter sp. REDSEA-S28_B2	2 687 817	63.7	8	浅海海水
3	GCA_001626515.1	Erythrobacter sp. REDSEA-S43_B2	2 871 770	63.6	18	浅海海水
4	GCA_003149575.1	Altererythrobacter sp. XM-24bin4	2 534 571	56.8	12	浅海海水
5	GCA_014983025.1	Erythrobacter sp. E2-1 Yellow Sea	2 518 489	57.4	31	浅海海水
6	GCA_017644245.1	Altererythrobacter sp. HCUG1430-4	2 509 776	56.8	9	浅海海水
7	GCF_000152865.1	Erythrobacter sp. NAP1	3 266 173	61.0	2	浅海海水
8	GCF_000176355.1	Alteriqipengyuania bathyomarina JL354	3 273 334	65.0	68	浅海海水
9	GCF_000186705.1	Alteriqipengyuania sp. JLT1363	3 117 324	64.9	26	浅海海水
10	GCF_000235975.1	Novosphingobium pentaromativorans US6-1	5 344 974	63.1	123	浅海沉积物
11	GCF_000767465.1	Novosphingobium pentaromativorans US6-1	5 457 578	63.0	6	浅海沉积物
12	GCF_000813185.1	Novosphingobium sp. MBES04	5 361 448	63.3	33	浅海海水
13	GCF_001008165.2	Croceibacterium atlanticum 26DY36	3 475 106	61.9	2	深海沉积物
14	GCF_001010925.1	Aurantiacibacter gangjinensis K7-2	2 724 959	62.7	8	浅海海水
15	GCF_001010945.1	Alteraurantiacibacter luteus KA37	2 887 216	67.2	22	浅海沉积物
16	GCF_001013305.1	Alteraurantiacibacter marinus HWDM-33	2 834 102	59.1	10	浅海海水
17	GCF_001021555.1	Alteraurantiacibacter marinus KCTC 23554	2 835 898	59.1	5	浅海海水
18	GCF_001028625.1	Pelagerythrobacter marensis KCTC 22370	2 885 033	64.7	1	浅海海水
19	GCF_001077815.2	Aurantiacibacter atlanticus s21-N3	3 226 359	58.3	2	深海沉积物
20	GCF_001281485.1	Altererythrobacter epoxidivorans CGMCC 1.7731	2 786 256	61.5	1	深海沉积物
21	GCF_001461885.1	Pelagerythrobacter marensis DSM 21428	2 902 055	64.7	14	浅海海水
22	GCF_001634625.1	Croceicoccus bisphenolivorans H4	3 602 538	62.8	74	浅海海水
23	GCF_001634715.1	Erythrobacter sp. HI0020	3 189 298	63.5	275	浅海海水
24	GCF_001635465.1	Erythrobacter sp. HI00D59	3 347 358	63.4	121	深海海水
25	GCF_001635685.1	Erythrobacter sp. HI0063	3 258 146	63.3	356	深海海水
26	GCF_001661675.2	Croceicoccus marinus E4A9	4 109 188	64.5	3	深海沉积物
27	GCF_001661915.1	Croceicoccus pelagius Ery9	3 306 530	62.8	40	浅海海水
28	GCF_001661965.1	Croceicoccus mobilis Ery22	4 210 079	62.5	32	深海沉积物
29	GCF_001663155.1	Altererythrobacter ishigakiensis NBRC 107699	2 673 978	56.9	1	浅海沉积物
30	GCF_001677335.1	Erythrobacter dokdonensis DSW-74	2 995 154	64.8	11	浅海海水
31	GCF_001687545.1	Paraurantiacibacter namhicola JCM 16345	2 591 679	65.0	1	浅海海水
32	GCF_001698205.1	Tsuneonella dongtanensis KCTC 22672	3 009 495	65.8	1	浅海沉积物
33	GCF_001719165.1	Erythrobacter litoralis DSM 8509	3 251 353	65.2	1	浅海海水
34	GCF_001886695.1	Aurantiacibacter gangjinensis JCM 15420	2 724 951	62.7	2	浅海海水
35	GCF_002155305.1	Erythrobacter dokdonensis DSM 17193	2 996 446	64.8	13	浅海海水
36	GCF_002155425.1	Erythrobacter donghaensis DSM 16220	3 372 281	66.2	11	浅海海水
37	GCF_002155655.1	Erythrobacter sanguineus JCM 20691	3 018 761	63.6	34	浅海海水
38	GCF_002237615.1	Qipengyuania flava VG1	3 184 307	63.3	1	浅海沉积物
39	GCF_002269345.1	Tsuneonella mangrovi C9-11	2 702 851	63.5	1	浅海沉积物
40	GCF_002556635.1	Novosphingobium sp. PC22D	5 024 522	65.8	57	深海海水
41	GCF_002795865.1	Qipengyuania seohaensis SW-135	2 942 673	61.7	1	浅海沉积物
42	GCF_002870965.1	Tsuneonella flava MS1-4	3 275 964	60.5	29	浅海沉积物
43	GCF_002895025.1	Erythrobacter sp. SAORIC-644	3 549 198	60.5	25	深海海水
44	GCF_003264115.1	Erythrobacter sp. KY5	3 311 272	60.8	1	浅海海水
45	GCF_003340475.1	Alteripontixanthobacter maritimus HME9302	2 676 898	60.8	2	浅海海水
46	GCF_003355455.1	Erythrobacter aureus YH-07	3 372 129	62.0	2	浅海沉积物
47	GCF_003363135.1	Alteriqipengyuania lutimaris S-5	3 290 764	65.4	12	浅海海水
48	GCF_003569745.1	Altererythrobacter sp. B11	3 842 046	65.4	1	深海沉积物
49	GCF_003584015.1	Aurantiacibacter xanthus CCTCC AB 2015396	4 380 877	64.5	146	浅海海水
50	GCF_003584125.1	Aurantiacibacter zhengii V18	3 803 809	62.7	29	深海沉积物
51	GCF_003605475.1	Aurantiacibacter aquimixticola JSSK-14	2 552 891	63.0	2	浅海海水
52	GCF_003605755.1	Tsuneonella suprasediminis Ery12	3 304 416	60.9	22	深海海水
53	GCF_004004515.1	Croceicoccus ponticola GM-16	3 244 470	62.1	17	浅海海水
54	GCF_004296255.1	Qipengyuania flava KJ5	2 819 202	63.9	1	浅海海水
55	GCF_004965515.1	Alteraurantiacibacter aquimixticola SSKS-13	3 433 901	63.9	5	浅海沉积物
56	GCF_005434975.1	Parerythrobacter lutipelagi GH1-16	3 097 651	60.6	2	浅海沉积物
57	GCF_005884405.1	Qipengyuania marisflavi KEM-5	2 669 685	61.7	18	浅海海水
58	GCF_006542605.1	Porphyrobacter sp. YT40	3 943 368	65.8	2	浅海海水
59	GCF_007004095.1	Erythrobacter insulae JBTF-M21	2 986 343	57.0	3	浅海海水
60	GCF_007570835.1	Croceicoccus sediminis S2-4-2	3 548 496	63.0	9	浅海沉积物
61	GCF_007830695.1	Altererythrobacter ishigakiensis ATCC BAA-2084	2 672 716	56.9	4	浅海沉积物
62	GCF_008107685.1	Novosphingobium sp. BW1	4 460 758	64.5	134	浅海沉积物
63	GCF_008932225.1	Qipengyuania flava 21-3	3 229 668	63.6	1	深海沉积物
64	GCF_009746585.1	Novosphingobium aquimarinum M24A2M	4 377 191	64.0	57	浅海海水
65	GCF_009827295.1	Qipengyuania pelagi JCM 17468	3 031 738	64.2	9	浅海海水
66	GCF_009827315.1	Qipengyuania gaetbuli DSM 16225	2 780 525	64.1	4	浅海沉积物
67	GCF_009827395.1	Pontixanthobacter aquaemixtae KCTC 52763	2 983 943	58.5	3	浅海海水
68	GCF_009827435.1	Croceibacterium salegens MCCC 1K01500	3 636 778	64.6	63	浅海沉积物
69	GCF_009827445.1	Qipengyuania aquimaris JCM 12189	2 662 510	61.8	1	浅海海水
70	GCF_009827455.1	Pontixanthobacter aestiaquae KCTC 42006	2 868 693	57.2	2	浅海海水
71	GCF_009827515.1	Pelagerythrobacter marinus H32	3 001 465	68.2	16	深海海水
72	GCF_009827535.1	Qipengyuania oceanensis MCCC 1A09965	2 874 198	63.9	14	深海沉积物
73	GCF_009827545.1	Pontixanthobacter gangjinensis JCM 17802	2 888 119	55.5	1	浅海沉积物
74	GCF_009827615.1	Allopontixanthobacter confluentis KCTC 52259	2 934 197	59.1	4	浅海海水
75	GCF_009827635.1	Parapontixanthobacter aurantiacus MCCC 1A09962	2 895 130	61.2	7	深海沉积物
76	GCF_009828015.1	Qipengyuania citrea CGMCC 1.8703	3 031 321	64.2	24	浅海海水
77	GCF_009828025.1	Qipengyuania algicida KEMB 9005-328	3 215 496	60.7	21	浅海海水
78	GCF_009828095.1	Pontixanthobacter luteolus SW-109	2 887 613	59.3	3	浅海海水
79	GCF_009828115.1	Allopontixanthobacter sediminis KCTC 42453	3 158 912	61.5	6	浅海沉积物
80	GCF_011762005.1	Qipengyuania flava DSM 16421	2 813 855	63.9	5	浅海海水
81	GCF_012972675.1	Pontixanthobacter rizhaonensis RZ02	2 790 898	55.5	4	浅海海水
82	GCF_012979275.1	Pseudopontixanthobacter vadosimaris JL3514	2 629 682	63.0	43	浅海海水
83	GCF_013260645.1	Erythrobacter mangrovi EB310	3 058 693	62.9	1	浅海沉积物
84	GCF_013370205.1	Actirhodobacter atriluteus HHU K3-1	2 939 611	62.1	23	浅海海水
85	GCF_013371495.1	Altererythrobacter lutimaris JGD-16	2 952 860	57.8	33	浅海海水
86	GCF_013408095.1	Novosphingobium marinum DSM 29043	3 591 024	64.2	22	深海海水
87	GCF_014191645.1	Alteriqipengyuania lutimaris CECT 8624	3 269 205	65.5	6	浅海沉积物
88	GCF_014199315.1	Croceibacterium atlanticum DSM 100738	3 457 495	61.9	22	深海沉积物
89	GCF_014640055.1	Novosphingobium marinum CGMCC 1.12918	3 590 209	64.2	27	深海海水
90	GCF_014641655.1	Alteriqipengyuania_A marina CGMCC 1.15297	2 634 902	64.9	56	浅海海水
91	GCF_014642495.1	Croceicoccus pelagius CGMCC 1.15358	3 228 892	62.8	42	浅海海水
92	GCF_014642595.1	Croceicoccus mobilis CGMCC 1.15360	4 116 752	62.6	37	深海沉积物
93	GCF_014645195.1	Novosphingobium indicum CGMCC 1.6784	4 961 652	63.0	103	深海沉积物
94	GCF_014705715.1	Erythrobacter rubeus KMU-140	3 042 712	60.6	4	浅海海水
95	GCF_015529805.1	Qipengyuania soli 6D36	2 910 144	63.3	1	浅海沉积物
96	GCF_015865035.1	Novosphingobium aureum YJ-S2-02	4 342 390	65.5	39	浅海沉积物
97	GCF_015999305.1	Erythrobacter sp. YJ-T3-07	3 317 720	64.3	494	浅海沉积物
98	GCF_016019885.1	Aurantiacibacter sediminis JGD-13	2 668 619	60.2	14	浅海沉积物
99	GCF_016745095.1	Croceicoccus sp. YJ47	3 353 209	64.4	1	深海海水
100	GCF_017312445.1	Novosphingobium sp. PY1	4 764 096	63.4	36	浅海沉积物
101	GCF_018205975.1	Erythrobacter sp. JK5	3 340 457	64.2	1	浅海沉积物
102	GCF_018398395.1	Croceicoccus gelatinilyticus 1NDH52	3 790 455	62.6	43	浅海沉积物
103	GCF_018417475.1	Novosphingobium decolorationis 502str22	4 750 375	65.5	2	深海沉积物
104	GCF_018636735.1	Erythrobacter sp. 3-20A1M	3 116 220	64.8	1	浅海海水
105	GCF_900115585.1	Qipengyuania nanhaisediminis CGMCC 1.7715	2 899 438	62.0	12	浅海沉积物
106	GCF_900143235.1	Erythrobacter sanguineus DSM 11032	2 978 798	63.8	25	浅海海水
107	GCF_900177715.1	Altererythrobacter xiamenensis CGMCC 1.12494	3 082 408	61.8	5	浅海海水

序号	GenBank登录号	菌株	基因组大小/bp	基因组GC含量/%	Contig数量/个	来源
1	GCA_001626395.1	Erythrobacter sp. REDSEA-S40_B1	2 688 120	63.7	7	浅海海水
2	GCA_001626415.1	Erythrobacter sp. REDSEA-S28_B2	2 687 817	63.7	8	浅海海水
3	GCA_001626515.1	Erythrobacter sp. REDSEA-S43_B2	2 871 770	63.6	18	浅海海水
4	GCA_003149575.1	Altererythrobacter sp. XM-24bin4	2 534 571	56.8	12	浅海海水
5	GCA_014983025.1	Erythrobacter sp. E2-1 Yellow Sea	2 518 489	57.4	31	浅海海水
6	GCA_017644245.1	Altererythrobacter sp. HCUG1430-4	2 509 776	56.8	9	浅海海水
7	GCF_000152865.1	Erythrobacter sp. NAP1	3 266 173	61.0	2	浅海海水
8	GCF_000176355.1	Alteriqipengyuania bathyomarina JL354	3 273 334	65.0	68	浅海海水
9	GCF_000186705.1	Alteriqipengyuania sp. JLT1363	3 117 324	64.9	26	浅海海水
10	GCF_000235975.1	Novosphingobium pentaromativorans US6-1	5 344 974	63.1	123	浅海沉积物
11	GCF_000767465.1	Novosphingobium pentaromativorans US6-1	5 457 578	63.0	6	浅海沉积物
12	GCF_000813185.1	Novosphingobium sp. MBES04	5 361 448	63.3	33	浅海海水
13	GCF_001008165.2	Croceibacterium atlanticum 26DY36	3 475 106	61.9	2	深海沉积物
14	GCF_001010925.1	Aurantiacibacter gangjinensis K7-2	2 724 959	62.7	8	浅海海水
15	GCF_001010945.1	Alteraurantiacibacter luteus KA37	2 887 216	67.2	22	浅海沉积物
16	GCF_001013305.1	Alteraurantiacibacter marinus HWDM-33	2 834 102	59.1	10	浅海海水
17	GCF_001021555.1	Alteraurantiacibacter marinus KCTC 23554	2 835 898	59.1	5	浅海海水
18	GCF_001028625.1	Pelagerythrobacter marensis KCTC 22370	2 885 033	64.7	1	浅海海水
19	GCF_001077815.2	Aurantiacibacter atlanticus s21-N3	3 226 359	58.3	2	深海沉积物
20	GCF_001281485.1	Altererythrobacter epoxidivorans CGMCC 1.7731	2 786 256	61.5	1	深海沉积物
21	GCF_001461885.1	Pelagerythrobacter marensis DSM 21428	2 902 055	64.7	14	浅海海水
22	GCF_001634625.1	Croceicoccus bisphenolivorans H4	3 602 538	62.8	74	浅海海水
23	GCF_001634715.1	Erythrobacter sp. HI0020	3 189 298	63.5	275	浅海海水
24	GCF_001635465.1	Erythrobacter sp. HI00D59	3 347 358	63.4	121	深海海水
25	GCF_001635685.1	Erythrobacter sp. HI0063	3 258 146	63.3	356	深海海水
26	GCF_001661675.2	Croceicoccus marinus E4A9	4 109 188	64.5	3	深海沉积物
27	GCF_001661915.1	Croceicoccus pelagius Ery9	3 306 530	62.8	40	浅海海水
28	GCF_001661965.1	Croceicoccus mobilis Ery22	4 210 079	62.5	32	深海沉积物
29	GCF_001663155.1	Altererythrobacter ishigakiensis NBRC 107699	2 673 978	56.9	1	浅海沉积物
30	GCF_001677335.1	Erythrobacter dokdonensis DSW-74	2 995 154	64.8	11	浅海海水
31	GCF_001687545.1	Paraurantiacibacter namhicola JCM 16345	2 591 679	65.0	1	浅海海水
32	GCF_001698205.1	Tsuneonella dongtanensis KCTC 22672	3 009 495	65.8	1	浅海沉积物
33	GCF_001719165.1	Erythrobacter litoralis DSM 8509	3 251 353	65.2	1	浅海海水
34	GCF_001886695.1	Aurantiacibacter gangjinensis JCM 15420	2 724 951	62.7	2	浅海海水
35	GCF_002155305.1	Erythrobacter dokdonensis DSM 17193	2 996 446	64.8	13	浅海海水
36	GCF_002155425.1	Erythrobacter donghaensis DSM 16220	3 372 281	66.2	11	浅海海水
37	GCF_002155655.1	Erythrobacter sanguineus JCM 20691	3 018 761	63.6	34	浅海海水
38	GCF_002237615.1	Qipengyuania flava VG1	3 184 307	63.3	1	浅海沉积物
39	GCF_002269345.1	Tsuneonella mangrovi C9-11	2 702 851	63.5	1	浅海沉积物
40	GCF_002556635.1	Novosphingobium sp. PC22D	5 024 522	65.8	57	深海海水
41	GCF_002795865.1	Qipengyuania seohaensis SW-135	2 942 673	61.7	1	浅海沉积物
42	GCF_002870965.1	Tsuneonella flava MS1-4	3 275 964	60.5	29	浅海沉积物
43	GCF_002895025.1	Erythrobacter sp. SAORIC-644	3 549 198	60.5	25	深海海水
44	GCF_003264115.1	Erythrobacter sp. KY5	3 311 272	60.8	1	浅海海水
45	GCF_003340475.1	Alteripontixanthobacter maritimus HME9302	2 676 898	60.8	2	浅海海水
46	GCF_003355455.1	Erythrobacter aureus YH-07	3 372 129	62.0	2	浅海沉积物
47	GCF_003363135.1	Alteriqipengyuania lutimaris S-5	3 290 764	65.4	12	浅海海水
48	GCF_003569745.1	Altererythrobacter sp. B11	3 842 046	65.4	1	深海沉积物
49	GCF_003584015.1	Aurantiacibacter xanthus CCTCC AB 2015396	4 380 877	64.5	146	浅海海水
50	GCF_003584125.1	Aurantiacibacter zhengii V18	3 803 809	62.7	29	深海沉积物
51	GCF_003605475.1	Aurantiacibacter aquimixticola JSSK-14	2 552 891	63.0	2	浅海海水
52	GCF_003605755.1	Tsuneonella suprasediminis Ery12	3 304 416	60.9	22	深海海水
53	GCF_004004515.1	Croceicoccus ponticola GM-16	3 244 470	62.1	17	浅海海水
54	GCF_004296255.1	Qipengyuania flava KJ5	2 819 202	63.9	1	浅海海水
55	GCF_004965515.1	Alteraurantiacibacter aquimixticola SSKS-13	3 433 901	63.9	5	浅海沉积物
56	GCF_005434975.1	Parerythrobacter lutipelagi GH1-16	3 097 651	60.6	2	浅海沉积物
57	GCF_005884405.1	Qipengyuania marisflavi KEM-5	2 669 685	61.7	18	浅海海水
58	GCF_006542605.1	Porphyrobacter sp. YT40	3 943 368	65.8	2	浅海海水
59	GCF_007004095.1	Erythrobacter insulae JBTF-M21	2 986 343	57.0	3	浅海海水
60	GCF_007570835.1	Croceicoccus sediminis S2-4-2	3 548 496	63.0	9	浅海沉积物
61	GCF_007830695.1	Altererythrobacter ishigakiensis ATCC BAA-2084	2 672 716	56.9	4	浅海沉积物
62	GCF_008107685.1	Novosphingobium sp. BW1	4 460 758	64.5	134	浅海沉积物
63	GCF_008932225.1	Qipengyuania flava 21-3	3 229 668	63.6	1	深海沉积物
64	GCF_009746585.1	Novosphingobium aquimarinum M24A2M	4 377 191	64.0	57	浅海海水
65	GCF_009827295.1	Qipengyuania pelagi JCM 17468	3 031 738	64.2	9	浅海海水
66	GCF_009827315.1	Qipengyuania gaetbuli DSM 16225	2 780 525	64.1	4	浅海沉积物
67	GCF_009827395.1	Pontixanthobacter aquaemixtae KCTC 52763	2 983 943	58.5	3	浅海海水
68	GCF_009827435.1	Croceibacterium salegens MCCC 1K01500	3 636 778	64.6	63	浅海沉积物
69	GCF_009827445.1	Qipengyuania aquimaris JCM 12189	2 662 510	61.8	1	浅海海水
70	GCF_009827455.1	Pontixanthobacter aestiaquae KCTC 42006	2 868 693	57.2	2	浅海海水
71	GCF_009827515.1	Pelagerythrobacter marinus H32	3 001 465	68.2	16	深海海水
72	GCF_009827535.1	Qipengyuania oceanensis MCCC 1A09965	2 874 198	63.9	14	深海沉积物
73	GCF_009827545.1	Pontixanthobacter gangjinensis JCM 17802	2 888 119	55.5	1	浅海沉积物
74	GCF_009827615.1	Allopontixanthobacter confluentis KCTC 52259	2 934 197	59.1	4	浅海海水
75	GCF_009827635.1	Parapontixanthobacter aurantiacus MCCC 1A09962	2 895 130	61.2	7	深海沉积物
76	GCF_009828015.1	Qipengyuania citrea CGMCC 1.8703	3 031 321	64.2	24	浅海海水
77	GCF_009828025.1	Qipengyuania algicida KEMB 9005-328	3 215 496	60.7	21	浅海海水
78	GCF_009828095.1	Pontixanthobacter luteolus SW-109	2 887 613	59.3	3	浅海海水
79	GCF_009828115.1	Allopontixanthobacter sediminis KCTC 42453	3 158 912	61.5	6	浅海沉积物
80	GCF_011762005.1	Qipengyuania flava DSM 16421	2 813 855	63.9	5	浅海海水
81	GCF_012972675.1	Pontixanthobacter rizhaonensis RZ02	2 790 898	55.5	4	浅海海水
82	GCF_012979275.1	Pseudopontixanthobacter vadosimaris JL3514	2 629 682	63.0	43	浅海海水
83	GCF_013260645.1	Erythrobacter mangrovi EB310	3 058 693	62.9	1	浅海沉积物
84	GCF_013370205.1	Actirhodobacter atriluteus HHU K3-1	2 939 611	62.1	23	浅海海水
85	GCF_013371495.1	Altererythrobacter lutimaris JGD-16	2 952 860	57.8	33	浅海海水
86	GCF_013408095.1	Novosphingobium marinum DSM 29043	3 591 024	64.2	22	深海海水
87	GCF_014191645.1	Alteriqipengyuania lutimaris CECT 8624	3 269 205	65.5	6	浅海沉积物
88	GCF_014199315.1	Croceibacterium atlanticum DSM 100738	3 457 495	61.9	22	深海沉积物
89	GCF_014640055.1	Novosphingobium marinum CGMCC 1.12918	3 590 209	64.2	27	深海海水
90	GCF_014641655.1	Alteriqipengyuania_A marina CGMCC 1.15297	2 634 902	64.9	56	浅海海水
91	GCF_014642495.1	Croceicoccus pelagius CGMCC 1.15358	3 228 892	62.8	42	浅海海水
92	GCF_014642595.1	Croceicoccus mobilis CGMCC 1.15360	4 116 752	62.6	37	深海沉积物
93	GCF_014645195.1	Novosphingobium indicum CGMCC 1.6784	4 961 652	63.0	103	深海沉积物
94	GCF_014705715.1	Erythrobacter rubeus KMU-140	3 042 712	60.6	4	浅海海水
95	GCF_015529805.1	Qipengyuania soli 6D36	2 910 144	63.3	1	浅海沉积物
96	GCF_015865035.1	Novosphingobium aureum YJ-S2-02	4 342 390	65.5	39	浅海沉积物
97	GCF_015999305.1	Erythrobacter sp. YJ-T3-07	3 317 720	64.3	494	浅海沉积物
98	GCF_016019885.1	Aurantiacibacter sediminis JGD-13	2 668 619	60.2	14	浅海沉积物
99	GCF_016745095.1	Croceicoccus sp. YJ47	3 353 209	64.4	1	深海海水
100	GCF_017312445.1	Novosphingobium sp. PY1	4 764 096	63.4	36	浅海沉积物
101	GCF_018205975.1	Erythrobacter sp. JK5	3 340 457	64.2	1	浅海沉积物
102	GCF_018398395.1	Croceicoccus gelatinilyticus 1NDH52	3 790 455	62.6	43	浅海沉积物
103	GCF_018417475.1	Novosphingobium decolorationis 502str22	4 750 375	65.5	2	深海沉积物
104	GCF_018636735.1	Erythrobacter sp. 3-20A1M	3 116 220	64.8	1	浅海海水
105	GCF_900115585.1	Qipengyuania nanhaisediminis CGMCC 1.7715	2 899 438	62.0	12	浅海沉积物
106	GCF_900143235.1	Erythrobacter sanguineus DSM 11032	2 978 798	63.8	25	浅海海水
107	GCF_900177715.1	Altererythrobacter xiamenensis CGMCC 1.12494	3 082 408	61.8	5	浅海海水