Journal of Marine Sciences ›› 2018, Vol. 36 ›› Issue (2): 27-35.DOI: 10.3969/j.issn.1001-909X.2018.02.004

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The influence of turbidity on the measurement of in vivo chlorophyll fluorescence and its correction method

YE Chen-jun1,2, PAN Jian-ming*1,2, ZHENG Min-hui1,2, XIE Shang-wei1,2, ZHANG Zhe-chao1,2   

  1. 1. Laboratory of Marine Ecosystem and Biogeochemistry,SOA,Hangzhou 310012,China;
    2. Second Institute of Oceanography,SOA,Hangzhou 310012,China
  • Received:2017-12-21 Revised:2018-03-27 Online:2018-06-15 Published:2022-11-21

Abstract: The influence of sediment turbidity on chlorophyll sensor determination of in vivo chlorophyll fluorescence was studied. The results show that turbidity can cause fluorescence effect and light shielding effect on the determination of chlorophyll fluorescence. In the range of 100 NTU turbidity, the fluorescence value of chlorophyll shows a good linear relationship with the turbidity of sediment suspension, which increases with the increase of turbidity. When actually measuring the in vivo chlorophyll fluorescence of diatoms in water, turbidity of sediment suspension causes light shielding effect that decreases in vivo chlorophyll fluorescence as turbidity increases. The concentration of microalgae in the water is higher, the light shielding effect is stronger. In 1 000 mV (including 10 μg/L), the highest concentration of microalgae, sediment suspension can reduce the chlorophyll fluorescence value by 20% at 100 NTU turbidity. Based on the experimental data, a correction method of turbidity effect of in vivo chlorophyll fluorescence detection is proposed. Within 100 NTU turbidity, the corrected deviation of chlorophyll fluorescence values is reduced to less than ±10% and the corrected fluorescence value is close to the true chlorophyll fluorescence.

Key words: Fluorometer, chlorophyll fluorescence, turbidity, sediment, correction method

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