Journal of Marine Sciences ›› 2020, Vol. 38 ›› Issue (2): 88-96.DOI: 10.3969/j.issn.1001-909X.2020.02.010

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Characteristics of eight isozymes inScomberomorus niphonius from Xiangshan Bay

ZHENG Baoxiao1, ZHENG Chunjing2, ZHANG Yu1, LOU Zhengjia1, LI Juan1, XUE Liangyi*1   

  1. 1. College of Marine Sciences, Ningbo University, Ningbo 315211, China;
    2. Ningbo Marine and Fishery Research Institute, Ningbo 315010, China
  • Received:2019-07-24 Online:2020-06-15 Published:2020-12-24

Abstract: In order to explore the biochemical and genetic characteristics of Scomberomorus niphonius, Gradient Polyacrylamide Gel Electrophoresis (GPGE) was used to analyze the expression patterns of eight isozymes (LDH, MDH, GDH, G6PDH, SOD, EST, SDH and MEP) in nine tissues (cardiac, gill, intestine, muscle, stomach, liver, eyes, kidney and gonad) of six individuals (3 females, 3 males) from Xiangshan Bay. The results showed that all eight isozymes were tissue-specific expression in nine tissues. LDH, MDH and G6PDH expressed in all nine tissues, while GDH only expressed little in the liver and eyes. Isoenzyme expressions were most abundant in liver among nine tissues. Eight isozymes were detected in the liver, in which the enzyme bands of EST were most abundant. GDH and G6PDH were more active in the liver than in other tissues. Isozyme expression patterns had no difference between male and female. The results provide a theoretical basis for the germplasm identification of Scomberomorus niphonius.

Key words: Scomberomorus niphonius, isoenzyme, tissue specificity

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