The variation of digestive enzyme activity in hepatopancreas of Portunus trituberculatus after molting

Abstract

Abstract: Digestive enzymes are important in reflecting the physiological state of digestion and the capacity of nutrition utilization. In the present study, digestive enzymes activity in hepatopancreas of Portunus trituberculatus after molting were studied. The results showed that pepsin, tryptase and amylase were the highest at 4~6 d after molting. Lipase activity reached the highest at 6 d after molting. Cellulase was the highest at 10~12 d after molting, though it maintained at a lower level in all the detected time. Chitinase was the highest at 4 d after molting. After molting, P. trituberculatus had high level of pepsin and tryptase activity, while the activity of cellulase and chitinase were low, which might be due to the characterization that P. trituberculatus mainly lived on animal derived food. The lipase activity in hepatopancreas was low, and it might be result from that the optimal determination condition was not provided. Compared to the other time, activity of all the detected digestive enzyme were high at 6 d after molting, which indicated that the crabs of this stage had higher food digestive capacity than that in other time. This result may help to provide reasonable reference for food providing in aquaculture of crab culture.

Key words: Portunus trituberculatus, molting, hepatopancreas, digestive enzyme activity

CLC Number:

S968.25

LIN Peng, LIU Hua, YU Zuo-ben, MU Chang-kao, SONG Wei-wei, LI Rong-hua, WANG Chun-Lin. The variation of digestive enzyme activity in hepatopancreas of Portunus trituberculatus after molting[J]. Journal of Marine Sciences, 2013, 31(4): 74-79.

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