Halophilic and halotolerant bacteria were isolated by media HM and ZMCA from seawater of ten sampling stations of the Changjiang River Estuary. Totally 156 16S rDNA sequences were obtained, of which 136 sequences were obtained from bacteria isolated by medium HM and the others from medium ZMCA. The bacteria from medium HM could be classified into 33 OUTs and they belonged to genus Oceanicola, Sagittula, Aurantimonas and Stappia within the class α-Proteobacteria; genus Alteromonas, Marinobacter and Pseudoalteromonas within the class γ-Proteobacteria and genus Bacillus, Gracilibacillus, Oceanobacillus, Halobacillus as well as Thalassobacillus within the class Bacilli of phylum Firmicutes. Strains isolated from stations M5-1, N6-2 and M4-1 which close to mainland were all belonged to Firmicutes, while strains isolated from stations far from mainland were all belonged to Proteobacteria. The bacteria isolated from medium ZMCA could be classified into 10 OUTs and they belonged to α-Proteobacteria and Actinobacteria. About 70.5% of the 136 strains isolated from medium HM could grow without NaCl and be classified as halotolerant bacteria. About 25.9% of the strains isolated from medium HM could grow in medium containing 0.5% NaCl but no growth was observed without NaCl and could be classified as moderately halophilic bacteria. While for the other 3.59% strains isolated from medium HM, no growth was observed in medium containing 0% or 0.5% NaCl, but growth was observed in media with 10% NaCl, which also could be classified as moderately halophilic bacteria. All strains isolated from medium ZMCA could grow without NaCl and be classified as halotolerant bacteria. 30.3% of strains isolated from medium HM could reduce nitrate, while only 9.9% of the strains could reduce nitrite.
Key words
Changjiang River Estuary /
halophilic bacteria /
halotolerant bacteria /
bacterial community
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