Effects of oxygen content and attachment area on extracellular polymeric substance from marine benthic diatom

CHEN Qi; WU Fu-yuan; ZHENG Ji-yong; YANG Jing-ya; LIN Cun-guo

doi:10.3969/j.issn.1001-909X.2017.01.008

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Journal of Marine Sciences ›› 2017, Vol. 35 ›› Issue (1) : 66-72. DOI: 10.3969/j.issn.1001-909X.2017.01.008

Effects of oxygen content and attachment area on extracellular polymeric substance from marine benthic diatom

CHEN Qi^1,3, WU Fu-yuan², ZHENG Ji-yong^*1,2, YANG Jing-ya², LIN Cun-guo^1,2

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Abstract

The extracellular polymeric substance (EPS) was secreted when marine benthic diatom attached on submerged surfaces, the environment factors had a directly impact on the composition and secretion action of EPS. The effects of the oxygen content and the attachment area on EPS were studied. The EPS from marine benthic diatoms were extracted by hot solvent method. The phenol-sulfuric acid method was employed to measure the content of polysaccharide and the Coomassie Brilliant Blue G-250 method was employed to measure the content of protein in the extractions. The cell number of diatoms was measured through the method of blood cell counting plate and UV spectrophotometry. The results show that the growth rate of diatoms is fast and the secretion amounts of EPS in a single cell are great under the higher oxygen content and larger attachment area conditions. The oxygen content has a great impact on the content of water-soluble polysaccharide as the main composition in EPS, while it has little impact on the else components. Compared to the oxygen content, the attachment area has little impact on the attachment and growth of diatoms.

Key words

marine benthic diatom / oxygen content / attachment area / extracellular polymeric substance

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CHEN Qi, WU Fu-yuan, ZHENG Ji-yong, YANG Jing-ya, LIN Cun-guo. Effects of oxygen content and attachment area on extracellular polymeric substance from marine benthic diatom[J]. Journal of Marine Sciences. 2017, 35(1): 66-72 https://doi.org/10.3969/j.issn.1001-909X.2017.01.008

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