Analysis on caroteniod producing of four marine Rhodococcus and its genome sequence determining

doi:10.3969/j.issn.1001-909X.2016.04.010

Abstract

Abstract: Rhodococcus sp. EPR-134, Rhodococcus sp. EPR-147, Rhodococcus sp. EPR-157 and Rhodococcus sp. EPR-279 were isolated from water samples of East Pacific Ocean, extraction of pigment and pigment full wavelength scan were carried out in this study. Based on the whole genome sequencing, the related genes in carotenoid metabolic pathways were analyzed. Pigment full wavelength scan results show that strain EPR-134 does not have a kind of caroteniod production ability, while other 3 strains of Rhodococcus can produce carotenoids, and the carotenoid composition was different. Genome analysis show that there are more complete gene clusters in the 4 bacterial genomes. crt E gene、crt B gene and crt I gene are very important in the metabolic pathway of caroteniod producing, and by analyzing amino acid sequence homology of these genes in different strains, it is find that amino acid sequences of these genes in the strain EPR-134 share the lowest sequence homology compared with other 3 strains. This may be the key reason why strain EPR-134 can't produce carotenoid. The results of this study may lay a preliminary foundation for genetic modification of the carotenoid producing Rhodococcus and construction of carotenoids producing engineering bacteria.

Key words: marine Rhodococcus, carotenoid, genome, metabolic pathway

CLC Number:

ZHANG Di, ZHANG Ning, CHEN Ji-gang, YANG Ji-fang. Analysis on caroteniod producing of four marine Rhodococcus and its genome sequence determining[J]. Journal of Marine Sciences, 2016, 34(4): 78-83.

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