Effects of exogenous salicylic acid on the growth and physiology of marine red algae Gracilaria lemaneiformis

Abstract

Abstract: To investigate the effects of different mass concentration of exogenous salicylic acid on the marine red algae Gracilaria lemaneiformis, the physiological parameters and the chlorophyll fluorescence characteristics of G. lemaneiformis were studied. Results showed that salicylic acid could increase the growth rate and the 10 μg·mL^-1 treatment group was better than other groups. The daily relative growth rate reached to 6.92%/d with a increase of 26.3% compared with the control group. When on the 3rd day, the 10 μg·mL^-1 treatment group of salicylic acid reached the maximum value with proline increased 32.5%, SOD increased 24.3%, and phycoerythrin increased 24.6% respectively. It also promoted the accumulation of Ca²⁺ and K⁺ in G. lemaneiformis. The chlorophyll fluorescence ΦPSⅡbegan to increase on the fifth day after 10 μg·mL^-1 treatment of salicylic acid and increased 9.6% compared with the control group. F_v/F_o reached a maximum on the fifth day, and increased 5.6% more than the control group. In conclusion, exogenous salicylic acid can enhance the growth of G. lemaneiformis and the 10 μg·mL^-1 treatment group of salicylic acid is the best concentration.

Key words: Gracilaria lemaneiformis, salicylic acid, physiology and biochemical, chlorophyll fluorescence

CLC Number:

S917.3

WANG Qiao-qiao, XU Nian-jun, ZHU Zhao-bo. Effects of exogenous salicylic acid on the growth and physiology of marine red algae Gracilaria lemaneiformis[J]. Journal of Marine Sciences, 2013, 31(2): 78-85.

References

[1] ZHOU Yi, YANG Hong-sheng, HU Hai-yan, et al. Bioremediation potential of macroalga Gracilaria lemaneiformis (Rhodophyta) integrated into fed fish culture in coastal waters of north China[J]. Aquaculture,2006,252(2-4):264-276.
[2] NELSON S G, GLENN E P, CONN J, et al. Cultivation of Gracilaria parvispora (Rhodophyta) in shrimp-farm effluent ditches and floating cages in Hawaii: A two-phase polyculture system[J]. Aquaculture,2001,193:239-248.
[3] ZHANG Xue-cheng, FEI Xiu-geng, WANG guang-ce, et al. Genetic studies and large scale cultivation of Gracilaria lemaneiformis[J]. Periodical of Ocean University of China,2009,39(5):947-954.
张学成,费修绠,王广策,等.江蓠属海藻龙须菜的基础研究与大规模栽培[J].中国海洋大学学报,2009,39(5):947-954.
[4] ZHU Zhao-bo, SUN Xue, XU Nian-jun, et al. Effects of salicylic acid on the resistance of Gracilaria/Gracilariopsis lemaneiformis to high temperature[J]. Journal of Fisheries of China,2012,36(8):1 304-1 312.
朱招波,孙雪,徐年军,等.水杨酸对龙须菜抗高温生理的影响[J].水产学报,2012,36(8):1 304-1 312.
[5] GAO Zheng-quan, MENG Chun-xiao, DIAO Ying-ying. The effect of extraneous salicylic acid on astaxanthin accumulation of alga Haematoccus pluvialis[J]. Fisheries Science,2007,26(7):377-380.
高政权,孟春晓,刁英英.施用水杨酸对雨生红球藻中虾青素积累的影响[J].水产科学,2007,26(7):377-380.
[6] CZERPAK R, BAJGUZ A, GROMEK M, et al. Activity of salicylic acid on the growth and biochemism of Chlorella vulgaris Beijerinck[J]. Acta Physiologiae Plantarum,2002,24(1):45-52.
[7] PROVASOLI L. Media and prospects for the cultivation of marine algae[C]//WATENABE A, HATTORI A. Culture and collection of algae. US-Japan Conference. Hakone: Japanese Society of Plant Physiology,1968:63-75.
[8] ABREU M H, VARELA D A, HENRIQUEZ L, et al. Traditional vs. integrated multi-trophic aquaculture of Gracilaria chilensis C. J. Bird, J. McLachlan & E. C. Oliveira: Productivity and physiological performance[J]. Aquaculture,2009,293:211-220.
[9] FENG Chen, LU Xin-zhi, YU Wen-gong. Biochemical and physiological effects of adversity stress on Porphyra Yezoensis[J]. Transactions of Oceanology and Limnology,2004(3):22-26.
冯琛,路新枝,于文功.逆境胁迫对条斑紫菜生理生化指标的影响[J].海洋湖沼通报,2004(3):22-26.
[10] LI He-sheng. The principle and technology of plant physiology & biochemistry[M]. Beijing: Higher Education Press,2000:67-168.
李合生.植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000:67-168.
[11] CAI Chun-er, HE Pei-min. Effects of three ammonium sulphate precipitation method on purification of crude phycobiliprotein[J]. Biotechnology Bulletin,2006(4):121-125.
蔡春尔,何培民.硫酸铵三步盐析对藻胆蛋白纯化的影响[J].生物技术通报,2006(4):121-125.
[12] SCHREIBER U, KLUGHAMMER C, KOLBOWSKI J. High-end chlorophyll fluorescence analysis with the MULTI-COLOR-PAM[J]. PAM Application Notes,2011,1:1-19.
[13] SLAYMAKER D H, NAVARRE D A, CLARK D, et al. The tobacco salicylic acid-binding protein 3(SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response[J]. PNAS,2002,99(18):11 640-11 645.
[14] FAN Wan-su, HUANG He-zhong, XU Han-fu, et al. Effects of exogenous salicylic acid on the resistance of Gracilaria lichevoides to chilling injury[J]. Marine Sciences,2011,35(2):38-43.
范皖苏,黄鹤忠,徐汗福,等.外源添加剂水杨酸对菊花江蓠抗寒性的影响[J].海洋科学,2011,35(2):38-43.
[15] ZHANG Xue-cheng, CHENG Xiao-jie, SUI Zheng-hong, et al. Study on phycobilisome of Gracilaria. I..Isolation and absorption spectral characteristics[J]. Journal of Ocean University of Qingdao,1999,29(2):265-270.
张学成,程晓杰,隋正红,等.江蓠属藻胆体的研究Ⅰ:藻胆体的分离及吸收光谱特性[J].青岛海洋大学学报,1999,29(2):265-270.
[16] KANG G Z, WANG C H, SUN G C. Salicylic acid changes activities of H₂O 2-metabolizing enzymes and increases the chilling tolerance of banana seedlings[J]. Environmental and Experimental Botany,2003,50:9-15.
[17] TAO Zong-ya, ZOU Qi, PENG Tao. Effects of salicylic acid on osmotic injury on wheat seedling leaves[J]. Journal of Plant Physiology,1999,19(2):296-302.
陶宗娅,邹琦,彭涛.水杨酸在小麦幼苗渗透胁迫中的作用[J].西北植物学报,1999,19(2):296-302.
[18] IWAMOTO K, SHIRAIWA Y. Salt-regulated mannitol metabolism in algae[J]. Marine Biotechnology,2005,7(5):407-415.
[19] ZHAO Li-ying, DENG Xi-ping, SHAN Lun. Effects of osmotic stress on chlorophyll fluorescence parameters of wheat seedling[J]. Chinese Journal of Applied Ecology,2005,16(7):1 261-1 264.
赵丽英,邓西平,山仑.渗透胁迫对小麦幼苗叶绿素荧光参数的影响[J].应用生态学报,2005,16(7):1 261-1 264.