Effects of CO2 and dissolved inorganic phosphate on the growth and photosynthetic performance of Ulva prolifera seedlings

doi:10.3969/j.issn.1001-909X.2023.02.010

Abstract

Abstract:

Micropropagules with rapid growth and reproduction are an important seed sources for the early outbreak of the green tide. In this study, the micropropagule seedlings of Ulva prolifera were collected as experimental materials, and two CO₂ levels (LC, 400 μatm; HC, 1 000 μatm) and three dissolved inorganic phoshphate (DIP) concentrations (LP, 0.32 μmol/L; MP, 3.62 μmol/L; HP, 36.2 μmol/L) were set to analyze the effects of CO₂, DIP and their interaction on its growth and photophysiological performance. The results of the growth indexes including relative growth rate (RGR), net photosynthetic rate (P_n) and respiration rate (R_d) showed that CO₂ significantly affected on the RGR and P_n (p<0.05). DIP significantly affected on the RGR, P_n and R_d (p<0.05). Specifically, RGR and P_n were significantly positively correlated with DIP concentration (p<0.05), while R_d was significantly negatively correlated with DIP concentration (p<0.05). However, their interaction had no significant effect on RGR, P_n and R_d (p>0.05). The results of fluorescence parameters including effective quantum yield [Y(II)], maximum relative electron transfer rate (rETR_max), saturation light intensity (E_k) and light utilization efficiency indicated (α) reveal that CO₂ significantly affected on the rETR_max and E_k (p<0.05), but had no significant effect on the Y(II) and α (p>0.05). DIP significantly affected all the fluorescence parameters, which were significantly promoted by the DIP concentration increase (p<0.05). And their interaction had significant effect on the rETR_max and Y(II) (p<0.05), but had no significant effect on the E_k and α (p>0.05). Pigments of Chl a, Chl b and carotenoid results showed that CO₂, DIP and their interaction significantly affected the pigments contents (p<0.05). Evelated-CO₂ significantly inhibited pigment synthesis (p<0.05), and the inhibitory effect was enhanced with the increasing of DIP concentration. The pigments contents were significantly positively correlated with DIP concentration (p<0.05). Our findings suggested that evelated-CO₂ and higher DIP concentration obviously promoted the growth of U.prolifera seedlings, which was favorable for the green tide outbreak.

Key words: Ulva prolifera, micropropagules, CO₂, dissolved inorganic phoshphate, growth

CLC Number:

S917.3

WANG Jinguo, SHENG Yangjie, WANG Xukun, NI Jiaxuan, WU Hui, LIU Weiguo, ZHOU Wei. Effects of CO₂ and dissolved inorganic phosphate on the growth and photosynthetic performance of Ulva prolifera seedlings[J]. Journal of Marine Sciences, 2023, 41(2): 114-122.

Figures/Tables 7

Tab.1 Parameters of seawater carbonate system under different treatments

处理组	pH	pCO₂/μatm	DIC/(μmol·kg^-1)	HC $O 3 -$ /(μmol·kg^-1)	C $O 3 2 -$ /(μmol·kg^-1)	CO₂/(μmol·kg^-1)	TA/(μmol·kg^-1)
LCLP	8.20±0.01^a	382.36±13.07^a	2 002.85±19.95^a	1 817.53±21.22^a	172.70±2.17^a	12.26±0.43^a	2 251.60±16.65^a
LCMP	8.18±0.02^a	408.82±24.69^a	2 036.32±33.82^a	1 854.54±36.41^a	168.29±3.41^a	13.49±0.81^a	2 276.87±27.12^a
LCHP	8.19±0.02^a	395.95±20.66^a	2 022.36±37.76^a	1 838.52±36.85^a	170.77±5.93^a	13.07±0.68^a	2 267.25±38.27^a
HCLP	7.85±0.00^b	931.28±15.51^b	2 092.77±34.85^b	1 978.08±32.94^b	83.96±1.40^b	30.73±0.51^b	2 194.97±35.74^a
HCMP	7.86±0.01^b	908.84±38.05^b	2 106.87±46.75^ab	1 989.78±44.75^b	87.09±2.30^b	30.00±1.26^b	2 214.06±46.52^a
HCHP	7.85±0.03^b	940.03±44.52^b	2 111.23±37.31^b	1 995.36±32.66^b	84.84±6.29^b	31.02±1.47^b	2 214.06±46.52^a

Tab.1 Parameters of seawater carbonate system under different treatments

处理组	pH	pCO₂/μatm	DIC/(μmol·kg^-1)	HC $O 3 -$ /(μmol·kg^-1)	C $O 3 2 -$ /(μmol·kg^-1)	CO₂/(μmol·kg^-1)	TA/(μmol·kg^-1)
LCLP	8.20±0.01^a	382.36±13.07^a	2 002.85±19.95^a	1 817.53±21.22^a	172.70±2.17^a	12.26±0.43^a	2 251.60±16.65^a
LCMP	8.18±0.02^a	408.82±24.69^a	2 036.32±33.82^a	1 854.54±36.41^a	168.29±3.41^a	13.49±0.81^a	2 276.87±27.12^a
LCHP	8.19±0.02^a	395.95±20.66^a	2 022.36±37.76^a	1 838.52±36.85^a	170.77±5.93^a	13.07±0.68^a	2 267.25±38.27^a
HCLP	7.85±0.00^b	931.28±15.51^b	2 092.77±34.85^b	1 978.08±32.94^b	83.96±1.40^b	30.73±0.51^b	2 194.97±35.74^a
HCMP	7.86±0.01^b	908.84±38.05^b	2 106.87±46.75^ab	1 989.78±44.75^b	87.09±2.30^b	30.00±1.26^b	2 214.06±46.52^a
HCHP	7.85±0.03^b	940.03±44.52^b	2 111.23±37.31^b	1 995.36±32.66^b	84.84±6.29^b	31.02±1.47^b	2 214.06±46.52^a

Fig.1 Relative growth rate of U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05), and different capital letters represent significant differences among different treatments under HC, p<0.05; Asterisk represents significant differences between LC and HC within a DIP treatment,p<0.05.)

Fig.2 Effective quantum yield of U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05), and different capital letters represent significant differences among different treatments under HC, p<0.05.)

Fig.3 Relative electron transfer rate of U.prolifera seedlings under different PAR

Tab.2 The maximum rETR (rETRmax), light utilization efficiency (α) and saturation light intensity (Ek) of U.prolifera seedlings under different treatments

处理组	最大相对光合电子传递速率 (rETR_max)/(μmol·m^-2·s^-1)	光能利用效率(α)	饱和光强(E_k)/ (μmol·m^-2·s^-1)
LCLP	61.89±3.12^a	0.29±0.02^a	211.38±6.35^a
LCMP	92.63±1.93^b	0.31±0.02^ab	302.23±23.16^b
LCHP	105.88±2.93^c	0.33±0.02^b	317.11±13.97^b
HCLP	48.39±1.28^A*	0.32±0.02^A	153.20±11.79^A*
HCMP	92.63±7.87^B	0.34±0.01^A	274.33±15.32^B
HCHP	104.79±6.13^B	0.35±0.03^A	303.19±38.09^B

Fig.4 Net photosynthetic rate (a) and respiration rate (b) of U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05, and different capital letters represent significant differences among different treatments under HC, p<0.05; Asterisk represents significant differences between LC and HC within a DIP treatment, p<0.05.)

Fig.5 The contents of chlorophyll a (a), chlorophyll b (b) and carotenoids (c) contents in U.prolifera seedlings under different treatments (Different lowercase letters represent significant differences among different treatments under LC, p<0.05, and different capital letters represent significant differences among different treatments under HC, p<0.05; Asterisk represents significant differences between LC and HC within a DIP treatment, p<0.05.)

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Effects of CO₂ and dissolved inorganic phosphate on the growth and photosynthetic performance of Ulva prolifera seedlings

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