Journal of Marine Sciences ›› 2023, Vol. 41 ›› Issue (2): 114-122.DOI: 10.3969/j.issn.1001-909X.2023.02.010

• Original article • Previous Articles     Next Articles

Effects of CO2 and dissolved inorganic phosphate on the growth and photosynthetic performance of Ulva prolifera seedlings

WANG Jinguo1,2,3(), SHENG Yangjie1,2,3,4, WANG Xukun1,2,3, NI Jiaxuan1,2,3, WU Hui1,2,3, LIU Weiguo5, ZHOU Wei1,2,3,5,*()   

  1. 1. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, China
    2. Key Laboratory of Coastal Salt Marsh Ecosystems and Resources, MNR, Lianyungang 222005, China
    3. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang 222005,China
    4. College of Science, Shantou University, Shantou 515000, China
    5. Rushan Oyster Association, Weihai 264500, China
  • Received:2022-04-02 Revised:2022-06-29 Online:2023-06-15 Published:2023-07-27


Micropropagules with rapid growth and reproduction are an important seed sources for the early outbreak of the green tide. In this study, the micropropagule seedlings of Ulva prolifera were collected as experimental materials, and two CO2 levels (LC, 400 μatm; HC, 1 000 μatm) and three dissolved inorganic phoshphate (DIP) concentrations (LP, 0.32 μmol/L; MP, 3.62 μmol/L; HP, 36.2 μmol/L) were set to analyze the effects of CO2, DIP and their interaction on its growth and photophysiological performance. The results of the growth indexes including relative growth rate (RGR), net photosynthetic rate (Pn) and respiration rate (Rd) showed that CO2 significantly affected on the RGR and Pn (p<0.05). DIP significantly affected on the RGR, Pn and Rd (p<0.05). Specifically, RGR and Pn were significantly positively correlated with DIP concentration (p<0.05), while Rd was significantly negatively correlated with DIP concentration (p<0.05). However, their interaction had no significant effect on RGR, Pn and Rd (p>0.05). The results of fluorescence parameters including effective quantum yield [Y(II)], maximum relative electron transfer rate (rETRmax), saturation light intensity (Ek) and light utilization efficiency indicated (α) reveal that CO2 significantly affected on the rETRmax and Ek (p<0.05), but had no significant effect on the Y(II) and α (p>0.05). DIP significantly affected all the fluorescence parameters, which were significantly promoted by the DIP concentration increase (p<0.05). And their interaction had significant effect on the rETRmax and Y(II) (p<0.05), but had no significant effect on the Ek and α (p>0.05). Pigments of Chl a, Chl b and carotenoid results showed that CO2, DIP and their interaction significantly affected the pigments contents (p<0.05). Evelated-CO2 significantly inhibited pigment synthesis (p<0.05), and the inhibitory effect was enhanced with the increasing of DIP concentration. The pigments contents were significantly positively correlated with DIP concentration (p<0.05). Our findings suggested that evelated-CO2 and higher DIP concentration obviously promoted the growth of U.prolifera seedlings, which was favorable for the green tide outbreak.

Key words: Ulva prolifera, micropropagules, CO2, dissolved inorganic phoshphate, growth

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