浙江洞头栽培的羊栖菜具有不同来源,通过定期测定5种不同品系羊栖菜(原始采集地分别是韩国、浙江洞头、浙江南麂列岛、浙江东极岛及广东汕头)的栽培性状,发现在生长阶段后期,各品系在株重和株高方面都存在显著性差异,原始亲本引自韩国的羊栖菜品系较其他品系具有更好的生长优势,可作为育苗选种目标品系。利用ISSR分子标记技术对这5种羊栖菜品系进行遗传多样性分析,结果显示,从36条引物中筛选出的11条引物,经PCR扩增得到219条羊栖菜DNA片段,其中多态性片段为129条,多态性条带比率达58.9%;5个羊栖菜品系之间的遗传距离为0.151 0~0.382 4,且聚在一起,同属的鼠尾藻在最外群。本研究从DNA分子水平上揭示了目前洞头养殖的羊栖菜品系遗传多态性偏低,为今后开展羊栖菜种质资源保护及其优良苗种的选育提供了一定的理论参考。
Abstract
There are diverse origins of cultivated Sargassum fusiforme (Harv.) Setch. in Dongtou, Zhejiang Province. In this paper, 5 strains of S. fusiforme were investigated, of which one came from Korea, three were collected from Dongtou, Nanji Island, Dongji Island of Zhejiang Province, and the last one was from Shantou, Guangdong Province. Significant differences were found among the five strains in the algal weight and height. The strain of S. fusiforme from Korea had better growth advantages than others, so it could be used as target strain for breeding. The genetic diversity of S. fusiforme was analyzed by ISSR makers. The genetic distances among the samples were calculated by Popgen 32 and the cluster analysis was performed by UPGMA method. The results showed that 11 ISSR primers could amplify polymorphic and informative bands from the 36 screened primers. Total 219 DNA bands were amplified, of which 129 were polymorphic and the percentage of polymorphic loci reached to 58.9%. The genetic distances of the 5 samples varied from 0.151 0 to 0.382 4. In the dendrogram, five strains of S. fusiforme clustered into one clade and S. thunbergii were in the outgroup. The study revealed that the genetic diversity of the cultivated S. fusiforme in Dongtou was low on the DNA molecular level, which provided theoretical reference for the developing genetic resources conservation and superior seed breeding of S. fusiforme in the future.
关键词
羊栖菜 /
栽培性状 /
ISSR /
遗传多样性
Key words
Sargassum fusiforme (Harv.) Setch. /
cultivated characteristics /
ISSR /
genetic diversity
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] ZENG Cheng-kui. The records of economic seaweeds in China[M].Beijing: Science Press,1962.
曾呈奎.中国经济海藻志[M].北京:科学出版社,1962.
[2] ZENG Cheng-kui, LU Bao-ren. The records of seaweeds in China[M]. Beijing: Science Press,2000:32-33.
曾呈奎,陆保仁.中国海藻志[M].北京:科学出版,2000:32-33.
[3] SUN Jian-zhang, FANG Jia-zhong, ZHU Zhi-feng. A preliminary study on the reproductive biology of Sargassum fusiforme[J]. Journal of Zhejiang College of Fisheries,1996,15(4):243-249.
孙建璋,方家仲,朱植丰.羊栖菜Sargassum fusiforme(Harv.)Setch.繁殖生物学的初步研究[J].浙江水产学院学报,1996,15(4):243-249.
[4] ZHANG Zhan, LIU Jian-guo, LIU Ji-dong. Study review of Hizikia fusiformis [J]. Marine Fisheries Research,2002,23(3):67-74.
张展,刘建国,刘吉东.羊栖菜的研究述评[J].海洋水产研究,2002,23(3):67-74.
[5] WANG Bing, LI Jing, MA Shu-bing, et al. The experimental study of Sargassum fusiforme (Harv.) Setch. on hypoglycemic effect[J]. Chinese Journal of Marine Drugs,2000(3):33-35.
王兵,李靖,马舒冰,等.羊栖菜多糖降血糖作用的实验研究[J].中国海洋药物,2000(3):33-35.
[6] LUO Qi-jun, LIN Shao-zhen. Sargassum fusiforme (Harv.) Setch.——The gold card of Dongtou Island[M]. Beijing: Ocean Press,2013:53.
骆其君,林少珍.羊栖菜——海岛洞头的金名片[M].北京:海洋出版社,2013:53.
[7] ZOU Ding-hui, GAO Kun-shan. Regulation of gamete release in the economic brown seaweed Hizikia fusiformis(Phaeophyta)[J]. Biotechnol Lett,2005,27:915-918.
[8] ZOU Ding-hui, GAO Kun-shan. Seasonal pattern of reproduction of Hizikia fusiforme (Sargassaceae, Phaeophyta) from Nanao Island, Shantou, China[J]. J Applied Phycology,2006,18(2):195-201.
[9] PANG Shao-jun, GAO Sun-qin, SUN Jian-zhang. Cultivation of the brown alga Hizikia fusiformis(Harvey) Okamura: controlled fertilization and early development of seedlings in raceway tanks in ambient light and temperature[J]. J Applied Phycology,2006,18(6):723-731.
[10] PANG Shao-jun, ZHANG Zu-hui. Cultivation of the brown alga Hizikia fusiformis(Harvey) Okamura: stress resistance of artificially raised young seedlings revealed by chlorophyll fluorescence measurement[J]. J Applied Phycology,2007,19(5):557-565.
[11] ZHANG Sheng-bang, MA Wei-feng, YU Ping. Extraction, purification and hydroxyl radical scavenging activity of polysaccharides from Sargassum fusiforme[J].Food Science,2009,30(18):192-195.
张胜帮,麻卫锋,于萍.羊栖菜多糖提取分离及其清除自由基的活性研究[J].食品科学,2009,30(18):192-195.
[12] LIU Shu-xing, ZHU Li-li, ZHANG Shu-meng. Study on drying technology of sargasum [J]. Journal of Shanxi University of Science & Technology,2011,29(6):16-20.
刘树兴,朱莉莉,张书猛.羊栖菜干燥工艺研究[J].陕西科技大学学报,2011,29(6):16-20.
[13] YU Shen-hui, DENG Yun-yan, YAO Jian-ting, et al. Population genetics of wild Sargassum fusiforme(Harv.) setch. (Sargassaceae, Phaeophyta) along China’s coast[J]. J Applied Phycology,2012,24(5):1 287-1 294.
[14] ZHAO Feng-juan, WANG Xiu-liang, LIU Ji-dong, et al. Population genetic structure of Sargassum thunbergii(Fucales, Phaeophyta) detected by RAPD and ISSR markers[J]. J Applied Phycology,2007,19(5):409-416.
[15] ZHAO Feng-juan, LIU Fu-li, LIU Ji-dong, et al. Genetic structure analysis of natural Sargassum muticum (Fucales, Phaeophyta) populations using RAPD and ISSR markers[J]. J Applied Phycology,2008,20(2):191-198.
[16] LÜ Hui, YAO Jian-ting, LIU Fu-li, et al. Construction of DNA fingerprint for Hizikia fusiformis(Harvey) Okamura (Sargassaceae, Phaeophyta) strains based on RAPD analysis[J]. Marine Science,2009,22(12):30-33.
吕慧,姚建婷,刘福利,等.羊栖菜养殖品系DNA指纹图谱的研究[J].海洋科学,2009,22(12):30-33.
[17] NEI M, LI W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc Natl Acad Sci USA,1979,76(10):5 269-5 273.
[18] DI Hong, CHEN Yi-Li, JIN Li-Ping. Genetic diversity analysis of Chinese main potato cultivars by RAPD and AFLP makers[J]. Acta Agronomica Sinica,2006,32(6):899-904.
邸宏,陈伊里,金黎平.RAPD和AFLP标记分析中国马铃薯主要品种的遗传多样性[J].作物学报,2006,32(6):899-904.
[19] YU Shen-hui. Molecular taxonomy of Sargassum fusiforme and Sargassum genus and population genetics of S. horneri and S.fusiforme[D]. Qingdao: Institute of Oceanology, Chinese Academy of Sciences,2012.
于深辉.羊栖菜分类地位及铜藻和羊栖菜种群遗传的研究[D].青岛:中国科学院海洋研究所,2012.
[20] LÜ Hui. Genetic and fingerprint analysis of the Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta) strains[D]. Qingdao: Institute of Oceanology, Chinese Academy of Sciences,2009.
吕慧.羊栖菜养殖品系DNA指纹分析及遗传变异的研究[D].青岛:中国科学院海洋研究所,2009.
基金
国家科技支撑计划课题资助(2011BAD13B08);国家海洋公益性行业科研专项经费项目资助(201105009);浙江省海洋环保项目资助(WZHX2012-10-116)
PDF(1119 KB)
