通过紫外分光光度计测定了海洋微生物需钠弧菌 Vibrio natriegens 的生长曲线,通过扫描电镜和原子力显微镜观测了该细菌在船舶用结构钢(DH32)钢样表面成膜过程及试样腐蚀形貌,探讨了生物膜的形成过程及其对材料表面腐蚀的影响。结果表明,生物膜的形成过程与微生物生命活动关系密切。根据对比暴露在菌液和无菌培养基中的试样表面形貌,发现细菌的附着及成膜过程的不均匀性,改变了DH32钢样表面的物理和化学状态。细菌附着区与周围形成的氧浓差电池,以及细菌新陈代谢主要产物对金属离子的络合,共同促进了试样局部腐蚀加速。
Abstract
In order to study the influence of the formation of biofilm on the corrosion of DH32 structural steel, the growth curve of a marine microorganism (Vibrio natriegens) was tested by UV-Vis spectra, the formation process of biofilm and the surface of the coupons were characterized by scanning electron microscope and atomic force microscope, respectively. The results show that the formation of biofilm closely associates with the growth of bacteria, the formation process of biofilm changes the chemical and physical state of the steel surface. Moreover, the formation of oxygen concentration cell and binding power of extracellular polymeric substances with metal ions greatly promotes the DH32 surface oxidation corrosion.
关键词
海洋微生物 /
生物膜 /
腐蚀
Key words
marine microorganism /
biofilm /
corrosion
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基金
国家重点基础研究发展计划(973计划)资助(2014CB643306);上海自然科学基金项目资助(14ZR1419800)
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