Cd2+对三疣梭子蟹的毒性效应研究

宋国林; 母昌考; 李荣华; 王春琳

doi:10.3969/j.issn.1001-909X.2016.01.008

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海洋学研究 ›› 2016, Vol. 34 ›› Issue (1) : 61-66. DOI: 10.3969/j.issn.1001-909X.2016.01.008

研究论文

Cd²⁺对三疣梭子蟹的毒性效应研究

宋国林, 母昌考^*, 李荣华, 王春琳

作者信息 +

Study on toxic effect of cadmium on Portunus trituberculatus

SONG Guo-lin, MU Chang-kao^*, LI Rong-hua, WANG Chun-lin

Author information +

文章历史 +

摘要

本研究以三疣梭子蟹Portunus trituberculatus为研究对象,通过急性和亚急性暴露试验,研究Cd²⁺对其肝胰腺和鳃的毒性效应。结果表明:Cd²⁺对三疣梭子蟹的48、72和96 h半致死质量浓度分别为6.55、4.33和3.12 mg/L,Cd²⁺对三疣梭子蟹的安全质量浓度为0.31 mg/L;在安全质量浓度和渔业水质标准质量浓度(<0.005 mg/L)下,随着暴露时间的延长,三疣梭子蟹的肝胰腺和鳃中HSP70的表达量都出现“先升后降”的趋势,与对照组相比,鳃中HSP70的表达量比肝胰腺的变化明显。在安全质量浓度以下,三疣梭子蟹的存活率并没有明显的变化,但是HSP70的诱导表达反映机体受到Cd²⁺的胁迫。因此HSP70基因可用作低质量浓度Cd²⁺污染检测的生物标志物。

Abstract

In this study, acute and sub-acute experiments were conducted to elucidate the toxic effect of cadmium on Portunus trituberculatus. The results show that the median lethal dose of cadmium are 6.55, 4.33 and 3.12 mg/L for 48 h,72 h and 96 h respectively, and the safe concentration of this metal is 0.31 mg/L. As the extension of exposure time, the expressions of HSP70 in hepatopancreas and gill are increased at the beginning, and then decreased significantly under the safe concentration and fishery water quality standard. Compared with control group, the expression of HSP70 in gill is more obvious than that in hepatopancreas. Under the safe concentration, the survival rate of Portunus trituberculatus is not changed obviously, but the excessive expression of HSP70 reflects that the crab is stressed by Cd²⁺ exposure, so HSP70 indicators can be reflected more sensitive than the median lethal concentration at low concentration of Cd²⁺pollution.

导出引用

宋国林, 母昌考, 李荣华, 王春琳. Cd²⁺对三疣梭子蟹的毒性效应研究[J]. 海洋学研究. 2016, 34(1): 61-66 https://doi.org/10.3969/j.issn.1001-909X.2016.01.008

SONG Guo-lin, MU Chang-kao, LI Rong-hua, WANG Chun-lin. Study on toxic effect of cadmium on Portunus trituberculatus[J]. Journal of Marine Sciences. 2016, 34(1): 61-66 https://doi.org/10.3969/j.issn.1001-909X.2016.01.008

中图分类号： S917.4

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