Cd2+对三疣梭子蟹的毒性效应研究

doi:10.3969/j.issn.1001-909X.2016.01.008

海洋学研究 ›› 2016, Vol. 34 ›› Issue (1): 61-66.DOI: 10.3969/j.issn.1001-909X.2016.01.008

Cd²⁺对三疣梭子蟹的毒性效应研究

宋国林, 母昌考^*, 李荣华, 王春琳

宁波大学海洋学院,浙江宁波 315211

收稿日期:2015-04-22 修回日期:2015-10-11 出版日期:2016-03-15 发布日期:2022-11-24
通讯作者: * 母昌考(1976-),男,副教授,主要从事甲壳动物养殖方面的研究。E-mail:muchangkao@nbu.edu.cn
作者简介:宋国林(1991-),男,甘肃秦安县人,主要从事水产动物健康养殖方面的研究。 E-mail:nbusgl@sina.cn
基金资助:
宁波市重大科技攻关计划项目资助(2013C11017);浙江省公益性技术应用研究计划资助(2011C33025)

Study on toxic effect of cadmium on Portunus trituberculatus

SONG Guo-lin, MU Chang-kao^*, LI Rong-hua, WANG Chun-lin

School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received:2015-04-22 Revised:2015-10-11 Online:2016-03-15 Published:2022-11-24

摘要/Abstract

摘要： 本研究以三疣梭子蟹Portunus trituberculatus为研究对象,通过急性和亚急性暴露试验,研究Cd²⁺对其肝胰腺和鳃的毒性效应。结果表明:Cd²⁺对三疣梭子蟹的48、72和96 h半致死质量浓度分别为6.55、4.33和3.12 mg/L,Cd²⁺对三疣梭子蟹的安全质量浓度为0.31 mg/L;在安全质量浓度和渔业水质标准质量浓度(<0.005 mg/L)下,随着暴露时间的延长,三疣梭子蟹的肝胰腺和鳃中HSP70的表达量都出现“先升后降”的趋势,与对照组相比,鳃中HSP70的表达量比肝胰腺的变化明显。在安全质量浓度以下,三疣梭子蟹的存活率并没有明显的变化,但是HSP70的诱导表达反映机体受到Cd²⁺的胁迫。因此HSP70基因可用作低质量浓度Cd²⁺污染检测的生物标志物。

关键词: 三疣梭子蟹, Cd²⁺, 急性毒性实验, HSP70

Abstract: In this study, acute and sub-acute experiments were conducted to elucidate the toxic effect of cadmium on Portunus trituberculatus. The results show that the median lethal dose of cadmium are 6.55, 4.33 and 3.12 mg/L for 48 h,72 h and 96 h respectively, and the safe concentration of this metal is 0.31 mg/L. As the extension of exposure time, the expressions of HSP70 in hepatopancreas and gill are increased at the beginning, and then decreased significantly under the safe concentration and fishery water quality standard. Compared with control group, the expression of HSP70 in gill is more obvious than that in hepatopancreas. Under the safe concentration, the survival rate of Portunus trituberculatus is not changed obviously, but the excessive expression of HSP70 reflects that the crab is stressed by Cd²⁺ exposure, so HSP70 indicators can be reflected more sensitive than the median lethal concentration at low concentration of Cd²⁺pollution.

Key words: Portunus trituberculatus, Cd²⁺, acute toxicity test, HSP70

中图分类号:

S917.4

宋国林, 母昌考, 李荣华, 王春琳. Cd²⁺对三疣梭子蟹的毒性效应研究[J]. 海洋学研究, 2016, 34(1): 61-66.

SONG Guo-lin, MU Chang-kao, LI Rong-hua, WANG Chun-lin. Study on toxic effect of cadmium on Portunus trituberculatus[J]. Journal of Marine Sciences, 2016, 34(1): 61-66.

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Cd²⁺对三疣梭子蟹的毒性效应研究

Study on toxic effect of cadmium on Portunus trituberculatus

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