利用气-质法(GC-MS)对越冬期间海水平均温度在20,16,12,10和8 ℃条件下的2龄大黄鱼肌肉和肝脏组织中脂肪酸组成及相对含量进行分析,结果表明:随着海水水温的降低,机体会增加不饱和脂肪酸含量,尤其是EPA[C20:5(n-3)],EPA在肌肉中的含量从20 ℃时5.71%增至8 ℃时8.06%,在肝脏中从20 ℃时6.76%增至8 ℃时8.44%。由此可见,在越冬禁食条件下,大黄鱼机体通过自身的脂肪酸动态转化和代谢,减少饱和脂肪酸含量,而增加不饱和脂肪酸含量,以此增加膜的流动性,抵御寒冷。
Abstract
Investigations were carried out to explore the changes of muscle and liver fatty acid composition and content in Pseudosciaena crocea during the winter. Gas chromatography-mass spectrometry (GC-MS) was used to detect fatty acid composition and content of its muscle and liver under the condition of sea water temperature at 20 ℃, 16 ℃, 12 ℃, 10 ℃ and 8 ℃ respectively. The results show that the content of unsaturated fatty acid increases with the reduction of water temperature, especially the content of EPA[C20:5(n-3)], from 5.71% at 20 ℃ to 8.06% at 8 ℃ in muscle and from 6.76% at 20 ℃ to 8.44% at 8 ℃ in liver. Therefore, with no feeding condition during the winter, saturated fatty acid decreases and unsaturated fatty acid increases through transformation and metabolism of fatty acid by organism itself to keep cell membranes fluidity and against the cold.
关键词
大黄鱼 /
越冬 /
脂肪酸
Key words
Pseudosciaena crocea /
winter /
fatty acid
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参考文献
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基金
国家高技术研究发展计划(“863”)项目资助(2012AA-200803-4);浙江省农业新品种选育重大专项资助(2012C12907-8);宁波市自然科学基金项目资助(2013A610162);宁波大学科研基金学科项目资助(xkw110)
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