外源水杨酸对龙须菜生长及生理的影响

海洋学研究 ›› 2013, Vol. 31 ›› Issue (2): 78-85.

外源水杨酸对龙须菜生长及生理的影响

王俏俏, 徐年军^*, 朱招波

宁波大学海洋学院,教育部应用海洋生物技术重点实验室,浙江省海洋生物工程重点实验室,浙江宁波 315211

收稿日期:2013-01-06 修回日期:2013-03-27 出版日期:2013-06-15 发布日期:2022-11-29
通讯作者: *徐年军,研究员,博士生导师,E-mail:xunianjun@nbu.edu.cn
作者简介:王俏俏(1987-),女,浙江衢州市人,硕士研究生,主要从事藻类生理生化方面的研究。E-mail:xiatiao11ya@126.com
基金资助:
国家自然科学基金资助项目(31072229);浙江省钱江人才资助项目(2007R10038)

Effects of exogenous salicylic acid on the growth and physiology of marine red algae Gracilaria lemaneiformis

WANG Qiao-qiao, XU Nian-jun^*, ZHU Zhao-bo

Key Laboratory of Applied Marine Biotechnology of the Ministry of Education,Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Science, Ningbo University, Ningbo 315211, China

Received:2013-01-06 Revised:2013-03-27 Online:2013-06-15 Published:2022-11-29

摘要/Abstract

摘要： 以龙须菜Gracilaria lemaneiformis为材料,研究了不同质量浓度水杨酸对龙须菜的生长速率、渗透调节物质、抗氧化酶、藻胆蛋白和叶绿素荧光动力学特性等的影响。结果表明,外施水杨酸可以促进龙须菜的生长,其中水杨酸质量浓度为10 μg/mL的处理组对促进龙须菜的生长效果最好,其日相对生长速率达到6.92%/d,比对照组的相对生长速率增长了26.3%。在用水杨酸处理后的第3天,水杨酸质量浓度为10 μg/mL处理组的各指标达到最大,脯氨酸增长了32.5%、SOD增长了24.3%、藻红蛋白增加了24.6%。同时水杨酸促进了Ca²⁺和K⁺离子在龙须菜藻体内的积累速率。用水杨酸处理后,叶绿素荧光参数ΦPSⅡ在处理后的第5天开始增长,比对照组增长了9.6%;F_v/F_o在第5天达到最大值,比对照组增长了5.6%。本研究表明,适当质量浓度的水杨酸有利于提高龙须菜的生长速率,其中水杨酸质量浓度为10 μg/mL的处理组对龙须菜的处理效果最好。

关键词: 龙须菜, 水杨酸, 生理生化, 叶绿素荧光

Abstract: To investigate the effects of different mass concentration of exogenous salicylic acid on the marine red algae Gracilaria lemaneiformis, the physiological parameters and the chlorophyll fluorescence characteristics of G. lemaneiformis were studied. Results showed that salicylic acid could increase the growth rate and the 10 μg·mL^-1 treatment group was better than other groups. The daily relative growth rate reached to 6.92%/d with a increase of 26.3% compared with the control group. When on the 3rd day, the 10 μg·mL^-1 treatment group of salicylic acid reached the maximum value with proline increased 32.5%, SOD increased 24.3%, and phycoerythrin increased 24.6% respectively. It also promoted the accumulation of Ca²⁺ and K⁺ in G. lemaneiformis. The chlorophyll fluorescence ΦPSⅡbegan to increase on the fifth day after 10 μg·mL^-1 treatment of salicylic acid and increased 9.6% compared with the control group. F_v/F_o reached a maximum on the fifth day, and increased 5.6% more than the control group. In conclusion, exogenous salicylic acid can enhance the growth of G. lemaneiformis and the 10 μg·mL^-1 treatment group of salicylic acid is the best concentration.

Key words: Gracilaria lemaneiformis, salicylic acid, physiology and biochemical, chlorophyll fluorescence

中图分类号:

S917.3

王俏俏, 徐年军, 朱招波. 外源水杨酸对龙须菜生长及生理的影响[J]. 海洋学研究, 2013, 31(2): 78-85.

WANG Qiao-qiao, XU Nian-jun, ZHU Zhao-bo. Effects of exogenous salicylic acid on the growth and physiology of marine red algae Gracilaria lemaneiformis[J]. Journal of Marine Sciences, 2013, 31(2): 78-85.

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