海水基质活性磷酸盐标准物质研制

张川; 于涛; 于小焱; 朱勇; 王丽芳; 张晓慧

doi:10.3969/j.issn.1001-909X.2023.03.009

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海洋学研究 ›› 2023, Vol. 41 ›› Issue (3) : 92-100. DOI: 10.3969/j.issn.1001-909X.2023.03.009

研究论文

海水基质活性磷酸盐标准物质研制

张川 ¹^,² ,
于涛 ¹^,² ,
于小焱 ¹^,² ,
朱勇 ³^,⁴ ,
王丽芳 ⁵ ,
张晓慧 ¹^,²

作者信息 +

Preparation of standard reference material for reactive phosphorus with seawater matrix

ZHANG Chuan ¹^,² ,
YU Tao ¹^,² ,
YU Xiaoyan ¹^,² ,
ZHU Yong ³^,⁴ ,
WANG Lifang ⁵ ,
ZHANG Xiaohui ¹^,²

Author information +

文章历史 +

摘要

本研究以西太平洋表层海水为基质,制备了浓度为0.5~4.0 μmol/L的海水活性磷酸盐系列标准物质。通过对所研制的4批次样品进行均匀性、稳定性评估,确定样品性能良好。对基于磷钼蓝分光光度法的气泡间隔连续流动分析系统的反应和操作条件进行优化,优化后的方法检出限低至0.03 μmol/L。不同浓度样品的相对标准偏差为0.37%~2.45%(n=9);通过与国际已有有证标准物质比对,本研究方法测量误差小于0.05 μmol/L。经6家具有中国计量认证资质且具备海水磷酸盐检测能力的实验室联合定值,确定了该系列标准物质的标准值,并对定值结果的不确定度进行了分析评价,相对扩展不确定度为2%~10%。

Abstract

Based on Western Pacific surface seawater as matrix, a series of reactive phosphorus reference materials with the concentration of 0.5-4.0 μmol/L were prepared. By investigating the uniformity and stability of the 4 batches of samples, it was confirmed that the performance met the needs of requirements. For the determination of reactive phosphorus in aqueous sample, segmented continuous flow analysis system with UV detector was used. The experimental conditions were optimized, the limit of detection(LOD) of the method was 0.03 μmol/L, and the relative standard deviation (RSD) of 4 different concentration batches was 0.37%-2.45%. The reliability of the method was verified by testing a CRM. The error was not more than 0.05 μmol/L. The concentration standard value of the series of reference substances was determined by 6 laboratories, and the relative expended uncertainty of characterization was evaluated, which was 2%-10%.

导出引用

张川, 于涛, 于小焱, 等. 海水基质活性磷酸盐标准物质研制[J]. 海洋学研究. 2023, 41(3): 92-100 https://doi.org/10.3969/j.issn.1001-909X.2023.03.009

ZHANG Chuan, YU Tao, YU Xiaoyan, et al. Preparation of standard reference material for reactive phosphorus with seawater matrix[J]. Journal of Marine Sciences. 2023, 41(3): 92-100 https://doi.org/10.3969/j.issn.1001-909X.2023.03.009

中图分类号： P734.44

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