Effects of oxygen content and attachment area on extracellular polymeric substance from marine benthic diatom

doi:10.3969/j.issn.1001-909X.2017.01.008

Abstract

Abstract: The extracellular polymeric substance (EPS) was secreted when marine benthic diatom attached on submerged surfaces, the environment factors had a directly impact on the composition and secretion action of EPS. The effects of the oxygen content and the attachment area on EPS were studied. The EPS from marine benthic diatoms were extracted by hot solvent method. The phenol-sulfuric acid method was employed to measure the content of polysaccharide and the Coomassie Brilliant Blue G-250 method was employed to measure the content of protein in the extractions. The cell number of diatoms was measured through the method of blood cell counting plate and UV spectrophotometry. The results show that the growth rate of diatoms is fast and the secretion amounts of EPS in a single cell are great under the higher oxygen content and larger attachment area conditions. The oxygen content has a great impact on the content of water-soluble polysaccharide as the main composition in EPS, while it has little impact on the else components. Compared to the oxygen content, the attachment area has little impact on the attachment and growth of diatoms.

Key words: marine benthic diatom, oxygen content, attachment area, extracellular polymeric substance

CLC Number:

U672.7⁺2

CHEN Qi, WU Fu-yuan, ZHENG Ji-yong, YANG Jing-ya, LIN Cun-guo. Effects of oxygen content and attachment area on extracellular polymeric substance from marine benthic diatom[J]. Journal of Marine Sciences, 2017, 35(1): 66-72.

References

[1] GAO Ya-hui, JING Hong-mei, HUANG De-qiang, et al. Advances in studies on the extracellular production of marine microalgae[J]. Marine Science,2002,26(3):35-38.
高亚辉,荆红梅,黄德强,等.海洋微藻胞外产物研究进展[J].海洋科学,2002,26(3):35-38.
[2] MARTIN J V, HILDER M, BRZEN M A. Silicon metabolism in diatoms: implications for growth[J]. Phycol,2003,36(8):821-840.
[3] HUANG Zong-guo, CAI Ru-xing. The marine biofouling organisms and prevention[M].Beijing: China Ocean Press,1984.
黄宗国,蔡如星.海洋污损生物及其防除[M].北京:海洋出版社,1984.
[4] ROUND F E, CRAWFORD R M, MANN D G. The Diatoms: Biology and morphology of the genera[M].Cambridge: Cambridge University Press,1990.
[5] CAO Shan. Research on adhesion mechanism and antifouling technologies of a marine benthic diatom[D]. Beijing: Tsinghua University,2013.
曹彬.海洋底栖硅藻附着机理及其防污技术研究[D].北京:清华大学,2013.
[6] CAO Shan, WANG Jia-dao, CHEN Hao-sheng, et al. Progress of marine biofouling and antifouling technologies[J]. Materials Science,2011,56(7):598-612.
[7] JING Hong-mei. Experimental studies on the extracellular products of marine microalgae[D]. Xiamen: Xiamen University,2001.
荆红梅.海洋微藻胞外产物的实验研究[D].厦门:厦门大学,2001.
[8] EGGE J, AKSNESK D. Silicate as regulating nutrient in phytoplankton competition[J]. Mar Ecol Prog Ser,1992,83(3):281-289.
[9] WUSTMAN B A, GERTZ M R, HOGLAND K D. Extracellular matrix assembly in diatoms(B)acillariophyceae, II. A model of adhesives based on chemical characterization and localization of polysaccharides from the marine diatom Achnantheslongipes[J]. Plant Physiology,1997,113(4):1 051-1 069.
[10] WANG Da-zhi, HUANG Shi-yu, CHENG Zhao-di. Studies on morphology,microstructure and component of extracellular polymeric substance from three species marine diatoms[J]. Oceanologia et Limnologia Sinica,2004,35(3):273-278.
王大志,黄世玉,程兆第.三种海洋硅藻胞外多聚物形态、微细结构及组成的初步研究[J].海洋与湖沼,2004,35(3):273-278.
[11] XU Gung-yu, YAN Jun, GUO Xiao-qiang. et al. The betterment and apply of Phenol-Sulphate Acid method[J]. Food Science,2005,26(8):342-346.
徐光域,颜军,郭晓强,等.硫酸-苯酚定糖法的改进与初步应用[J].食品科学,2005,26(8):342-346.
[12] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem,1976,72(1):248.
[13] WANG Xiao-ping, XING Shu-li. Determination of protein content by Coomassie Brilliant Blue method[J]. Chemical industry of Tianjin,2009,23(3):40-41.
王孝平,邢树礼.考马斯亮蓝法测定蛋白质含量的研究[J].天津化工,2009,23(3):40-41.
[14] ZOU Ning, SUN Dong-hong, GUO Xiao-yan. Effects of environmental and nutritional conditions on growth of marine benthic diatoms[J]. Journal of Aquaculture,2005,26(5):004.
邹宁,孙东红,郭小燕.培养条件对底栖硅藻生长的影响[J].水产养殖,2005,26(5):004.
[15] ALBERT R S, SNYDR S, ZAHURANEC B J, et al. Biofouling research needs for the United State Navy: Progam history and goals[J]. Biofouling,1992,6(2):91-95.
[16] DUBOIS M, GILLES K A, HAMILTON J K, et al. Colorimetric method for determination of sugars and related substances[J]. Anla Chem,1956,28(3):350-356.
[17] ZHANG Shao-yun. Studies on the mechanism and prevention properties of Ceramide for marine fouling organisms[D]. Qingdao: Ocean University of China,2012.
张少云.神经酰胺类物质对海洋污损生物的防除性能及其作用原理研究[D].青岛:中国海洋大学,2012.
[18] ZHAO Shou-huan. Studies on the inhibited effects of paeonol and four types of microstructure silica gel on Navicula minima[D]. Shanghai: Shanghai Ocean University,2015.
赵守涣.丹皮酚和四种微结构硅胶材料对小舟形藻的抑制作用研究[D].上海:上海海洋大学,2015.
[19] HOAGLAND K D, ROSOWSKI J R, GRETZ M R, et al. Diatom extracellular polymeric substances: function, fine structure chemistry, and physiology[J]. Journal of Phycology,2003,29(5):537-566.
[20] CHIOVITTI T M, WETHERBEE R. Diatom adhesives: molecular and mechanical properties[M]//SMITH A M, CALLOW J A. Biological adhesives[M]. Heidelberg: Springer,2006:79-103.
[21] UNDERWOOD G J C, PATERSON D M. The importance of extracellular carbohydrate production by marine epipelicdiatoms[J].Advances in Botanical Research,2003,40:183-240.
[22] MOLINOP J, WETHERBEE R. The biology of biofouling diatoms and their role in the development of microbial slimes[J]. Biofouling,2008,24(5):365-379.