Optical dissolved oxygen sensor based on fluorescence quenching principle can obtain spatiotemporal data of dissolved oxygen in seawater, which is widely used in long-term monitoring of marine environment. However, due to the “storage drift” and “measurement drift” occurring during storage and distribution, it is necessary to calibrate and correct the data drift of the instrument. Existing calibration methods have long cycle and complex operation. In this project, the response characteristics of optical dissolved oxygen sensor in air and water medium were studied, and a field calibration method of optical dissolved oxygen sensor using air medium was proposed, and a two-point calibration model of the instrument was established. Experimental results show that the calibration method can effectively correct the data drift of the dissolved oxygen sensor, and the deviation between the measured value of the sensor and the standard value of Winkler titration is within  ±6 μmol·L-1. This method can realize the convenient and rapid correction of optical dissolved oxygen sensor, avoid the tedious steps of laboratory maintenance, improve the quality of monitoring data, maintain the continuity of monitoring data, and has important application value.