长江口可培养耐(嗜)盐细菌群落结构研究

doi:10.3969/j.issn.1001-909X.2014.01.011

海洋学研究 ›› 2014, Vol. 32 ›› Issue (1): 90-97.DOI: 10.3969/j.issn.1001-909X.2014.01.011

长江口可培养耐(嗜)盐细菌群落结构研究

孟凡旭^1,2, 吴月红^1,2, 金文静³, 王春生^1,2, 许学伟^1,2

1.国家海洋局海洋生态系统与生物地球化学重点实验室,浙江杭州 310012;
2.国家海洋局第二海洋研究所,浙江杭州 310012;
3.浙江海洋学院萧山校区基础部,浙江杭州 311231

收稿日期:2013-07-17 修回日期:2014-01-22 出版日期:2014-03-15 发布日期:2022-11-25
作者简介:孟凡旭(1978-),男,河北玉田县人,博士,助理研究员,主要从事海洋微生物资源与应用方面的研究。E-mail: fanxumeng@sio.org.cn
基金资助:
国家海洋局第二海洋研究所基本科研业务费专项资金项目资助(JT1011,JG1025);中国近海海洋综合调查与评价项目(908-ZC-I-02)

Community of cultivable halophilic and halotolerant bacteria of the Changjiang River Estuary

MENG Fan-xu^1,2, WU Yue-hong^1,2, JIN Wen-jing³, WANG Chun-sheng^1,2, XU Xue-wei^1,2

1. Laboratory of Marine Ecosystem and Biogeochemistry, SOA, Hangzhou 310012, China;
2. The Second Institute of Oceanography, SOA, Hangzhou 310012, China;
3. Department of Basic Courses, Xiaoshan Campus,Zhejiang Ocean University, Hangzhou 311231, China

Received:2013-07-17 Revised:2014-01-22 Online:2014-03-15 Published:2022-11-25

摘要/Abstract

摘要： 针对长江口10个站位样品,采用HM培养基和ZMCA培养基分离培养耐(嗜)盐菌,通过PCR方法获取156株细菌的16S rDNA序列。其中,由HM培养基分离获得136株菌株,可划分为33个分类单元(OUT),分属α-变形菌纲(Alphaproteobacteria)、γ-变形菌纲(Gammaproteobacteria)和厚壁菌门(Firmicutes)的芽孢杆菌纲(Bacilli)3个类群,其中γ-变形菌纲为优势耐(嗜)盐细菌;靠近海岸的站点M5-1,N6-2和M4-1耐(嗜)盐细菌均属于厚壁菌门,而远离海岸的站点耐(嗜)盐细菌均属于变形菌门。以ZMCA培养基分离获得的20株菌株分属α-变形菌纲和放线菌纲(Actinobacteria),可划分为10个OTU。由HM培养基分离获得的菌株中,70.5%属于耐盐菌,29.5%属于中度嗜盐菌;由ZMCA培养基分离获得的菌株均为耐盐菌。HM培养基分离得到的136株细菌的硝酸盐和亚硝酸盐还原性分析表明,亚硝酸盐还原菌数量仅为硝酸盐还原菌的1/3左右。

关键词: 长江口, 耐盐菌, 嗜盐菌, 细菌群落

Abstract: Halophilic and halotolerant bacteria were isolated by media HM and ZMCA from seawater of ten sampling stations of the Changjiang River Estuary. Totally 156 16S rDNA sequences were obtained, of which 136 sequences were obtained from bacteria isolated by medium HM and the others from medium ZMCA. The bacteria from medium HM could be classified into 33 OUTs and they belonged to genus Oceanicola, Sagittula, Aurantimonas and Stappia within the class α-Proteobacteria; genus Alteromonas, Marinobacter and Pseudoalteromonas within the class γ-Proteobacteria and genus Bacillus, Gracilibacillus, Oceanobacillus, Halobacillus as well as Thalassobacillus within the class Bacilli of phylum Firmicutes. Strains isolated from stations M5-1, N6-2 and M4-1 which close to mainland were all belonged to Firmicutes, while strains isolated from stations far from mainland were all belonged to Proteobacteria. The bacteria isolated from medium ZMCA could be classified into 10 OUTs and they belonged to α-Proteobacteria and Actinobacteria. About 70.5% of the 136 strains isolated from medium HM could grow without NaCl and be classified as halotolerant bacteria. About 25.9% of the strains isolated from medium HM could grow in medium containing 0.5% NaCl but no growth was observed without NaCl and could be classified as moderately halophilic bacteria. While for the other 3.59% strains isolated from medium HM, no growth was observed in medium containing 0% or 0.5% NaCl, but growth was observed in media with 10% NaCl, which also could be classified as moderately halophilic bacteria. All strains isolated from medium ZMCA could grow without NaCl and be classified as halotolerant bacteria. 30.3% of strains isolated from medium HM could reduce nitrate, while only 9.9% of the strains could reduce nitrite.

Key words: Changjiang River Estuary, halophilic bacteria, halotolerant bacteria, bacterial community

中图分类号:

Q938.8

孟凡旭, 吴月红, 金文静, 王春生, 许学伟. 长江口可培养耐(嗜)盐细菌群落结构研究[J]. 海洋学研究, 2014, 32(1): 90-97.

MENG Fan-xu, WU Yue-hong, JIN Wen-jing, WANG Chun-sheng, XU Xue-wei. Community of cultivable halophilic and halotolerant bacteria of the Changjiang River Estuary[J]. Journal of Marine Sciences, 2014, 32(1): 90-97.

参考文献

[1] LU Jing-rang, DOMINGO J W S, LAMENDELLA R, et a1. Phylogenetic diversity and molecular detection of bacteria in gull feces[J]. Applied and Environmental Microbiology,2008,74(13):3 969-3 976.
[2] NING Xiu-ren, KUTI C. The relationship between Chl a, bacteria, ATP, POC and oxygen uptake rates of microorganism in Changjiang River Estuary and diluted water area[J]. Acta Oceanologica Sinica,1991,13(6):831-837.
宁修仁,C.库蒂.长江口及冲淡水区叶绿素a、细菌、ATP、POC及微生物呼吸作用速率之间的关系[J].海洋学报,1991,13(6):831-837.
[3] BACELAR-NICOLAU P, NICOLAU L B, MARQUES J C, et a1. Bacterioplankton dynamics in the Mondego estuary (Portugal) [J]. Acta Oecologica,2003,24(S1):67-75.
[4] LI Yun, LI Dao-ji. Correlation between the distribution of bacterioplankton and the environmental factors in the Yangtze River Estuary and its adjacent seas[J]. Marine Science Bulletin,2007,26(6):9-18.
李云,李道季.长江口邻近海域浮游细菌分布与环境因子的关系[J].海洋通报,2007,26(6):9-18.
[5] KUSHNER D J. Growth and nutrition of halophilic bacteria[M] // VREELAND R H, HOCHSTEIN L I.The biology of halophilic bacteria. Boca Raton, FL :CRC Press,1993:87-103.
[6] VENTOSA A, NIETO J J, OREN A. Biology of moderately halophilic aerobic bacteria[J]. Microbiology and Molecular Biology Reviews,1998,62(2):504-544.
[7] SONG Zhi-gang, XU Qiang-zhi, LU Xin-an, et al. A primary study on population biodiversity of marine microorganisms from East China Sea[J]. Microbiology China, 2006,33(1):63-67.
宋志刚,许强芝,鲁心安,等,中国东海海洋微生物多样性初步研究[J].微生物学通报,2006,33(1):63-67.
[8] LI He-yang, CHEN Ming-xia, JIAO Nian-zhi. The community composition of bacterioplankton at typical station in the Changjiang Estuary[J]. Acta Oceanologica Sinica,2012,34(1):183-187.
李和阳,陈明霞,焦念志.长江口典型站位浮游细菌类群的组成特征研究[J].海洋学报,2012,34(1):183-187.
[9] SEKIGUCHI H, KOSHIKAWA H, HIROKI M, et a1. Bacterial distribution and phylogenetic diversity in the Changjiang estuary before the construction[J]. Microbial Ecology,2002,43(1):82-91.
[10] ZOBELL C E. Studies on marine bacteria. I: The cultural requirements of heterotrophic aerobes[J]. Journal of Marine Research,1941,4:42-75.
[11] UPHOFF H U, FELSKE A, FEHR W, et al. The microbial diversity in picoplankton enrichment cultures: A molecular screening of marine isolates[J]. FEMS Microbiology Ecology,2001,35(3):249-258.
[12] RAVENSCHLAG K, SAHM K, PERNTHALER J, et a1. High bacterial diversity in permanently cold marine sediments[J]. Applied and Environmental Microbiology,1999,65(9):3 982- 3 989.
[13] XU Xue-wei, WU Yue-hong, WANG Chun-sheng, et al. Marinobacter pelagius sp. nov., a moderately halophilic bacterium[J]. International Journal of Systematic and Evolutionary,2008,58(3):637-640.
[14] CAI Wen-wei, WANG Hui, TIAN Yun, et al. Influence of a bacteriophage on the population dynamics of toxic dinoflagellates by lysis of algicidal bacteria[J]. Applied and Environmental Microbiology,2011,77(21):7 837-7 840.
[15] ZHENG Tian-ling, YANG Xiao-ru, SU Jian-qiang, et al. Compound with high algae removal activity and preparation method thereof: China, CN101935320A [P]. 2011-01-05.
郑天凌,杨小茹,苏建强,等.一种强杀藻活性化合物及其制备方法:中国,CN101935320A[P].2011-01-05.

长江口可培养耐(嗜)盐细菌群落结构研究

Community of cultivable halophilic and halotolerant bacteria of the Changjiang River Estuary

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	李玉洁, 王小谷, 林施泉, 王春生, . 长江口及邻近陆架海区夏季小型底栖动物群落结构研究[J]. 海洋学研究, 2022, 40(2): 102-112.
[2]	曾定勇, 宣基亮, 黄大吉, 等. 长江口海域潮汐和潮流的观测研究[J]. 海洋学研究, 2022, 40(1): 12-20.
[3]	栾奎峰, 徐航, 潘与佳, 等. 基于高光谱传感器的长江口表层悬浮泥沙质量浓度光谱曲线特征研究#br#[J]. 海洋学研究, 2022, 40(1): 64-71.
[4]	高抒. 长江口与东海陆架泥质沉积动力过程与环境效应：长期数据采集需求[J]. 海洋学研究, 2021, 39(4): 1-10.
[5]	周锋, 钱周奕, 刘安琪, 马晓, 倪晓波, 曾定勇, . 长江口及邻近海域底层水体低氧物理机制的研究进展[J]. 海洋学研究, 2021, 39(4): 17-38.
[6]	王斌, 刘希真, 李林蔚, 李德望, 金海燕, 高生泉, 李宏亮, 陈建芳, . 2011年长江口-东海P-PN断面碳酸盐体系参数的季节分布特征及影响因素[J]. 海洋学研究, 2021, 39(4): 39-51.
[7]	李德望, 林华, 陈思杨, 王斌, 王奎, 王尧, 马云龙, 金海燕, 陈建芳, . 2017年春季长江口-东海连续体pCO2的空间分布特征及其控制因素[J]. 海洋学研究, 2021, 39(4): 52-62.
[8]	杨旭锋, 于培松, 潘建明, 朱元励, 张偲, 张慧娟, . 2019年夏末长江口及其邻近海域走航pCO2变化及控制机制[J]. 海洋学研究, 2021, 39(4): 63-72.
[9]	罗颖, 金海燕, 李德望, 季仲强, 王斌, 杨志, 张扬, 庄燕培, 李杨杰, 陈建芳, 郑侦明, . 夏季洪水对长江口及邻近海域颗粒有机碳分布与来源的影响[J]. 海洋学研究, 2021, 39(4): 73-81.
[10]	孙振皓, 盛留洋, 江新琴, 马晓, 王斌, 曾江宁, 江志兵, . 2017年夏季长江口浮游植物暖水种增多现象分析[J]. 海洋学研究, 2021, 39(4): 82-90.
[11]	叶文建, 杜萍, 寿鹿, . 夏季长江口缺氧区、低氧区和正常溶解氧海区浮游动物群落特征比较研究[J]. 海洋学研究, 2021, 39(4): 91-100.
[12]	贾海波, 柴小平, 黄备. 2016—2019年长江口海域季节性低氧对大型底栖动物群落的影响[J]. 海洋学研究, 2021, 39(2): 80-88.
[13]	苗燕熠, 王斌, 李德望, 金海燕, 江志兵, 马晓, 于培松, 陈建芳, 王俊洋. 大风事件对长江口及邻近海域海-气CO₂通量的影响[J]. 海洋学研究, 2020, 38(1): 42-49.
[14]	任剑波, 何青, 沈健, 郭磊城, 徐凡. 远区台风“三巴”对河口波浪增水和波生流影响数值模拟研究[J]. 海洋学研究, 2019, 37(3): 21-30.
[15]	李晓慧, 刘镇盛. 长江口及邻近海域浮游动物生物量分布及季节变化[J]. 海洋学研究, 2017, 35(4): 94-101.