夏季长江口不同粒级浮游植物碱性磷酸酶活性

doi:10.3969j.issn.1001-909X.2022.04.003

海洋学研究 ›› 2022, Vol. 40 ›› Issue (4): 25-37.DOI: 10.3969j.issn.1001-909X.2022.04.003

夏季长江口不同粒级浮游植物碱性磷酸酶活性

王奕恒^1,2,3, 郝锵^*2,3,4, 陈建芳^1,2,3,4, 朱元励^2,3, 金海燕^1,2,3,4, 周锋^1,3,4, 张伟^2,3,5

1.浙江大学海洋学院,浙江舟山 316021;
2.自然资源部海洋生态系统动力学重点实验室,浙江杭州 310012;
3.自然资源部第二海洋研究所,浙江杭州 310012;
4.卫星海洋环境动力学国家重点实验室,浙江杭州 310012;
5.浙江省近岸工程环境与生态安全重点实验室,浙江杭州 310012

收稿日期:2022-06-07 出版日期:2022-12-15 发布日期:2023-02-09
通讯作者: ^*郝锵(1979—),男,副研究员,主要从事海洋初级生产力研究,E-mail:haoq@sio.org.cn。
作者简介:王奕恒(1997—),男,浙江省岱山县人,主要从事海洋生物地球化学研究,E-mail:wang0206@zju.edu.cn。
基金资助:
国家自然科学基金-浙江两化融合联合基金(U1709201);国家自然科学基金(41876026);浙江省万人计划项目(2020R52038);“全球变化与海气相互作用”专项二期任务(长江口缺氧酸化预警监测);中央级公益性科研院所基本科研业务费专项资金资助项目(SZ2001,JG1103)

Alkaline phosphatase activity of size-fractionated phytoplankton in the Changjiang Estuary in summer

WANG Yiheng^1,2,3, HAO Qiang^*2,3,4, CHEN Jianfang^1,2,3,4, ZHU Yuanli^2,3, JIN Haiyan^1,2,3,4, ZHOU Feng^1,3,4, ZHANG Wei^2,3,5

1. Ocean College, Zhejiang University, Zhoushan 316021, China;
2. Key Laboratory of Marine Ecosystem Dynamics, MNR, Hangzhou 310012, China;
3. Second Institute of Oceanography, MNR, Hangzhou 310012, China;
4. State Key Laboratory of Satellite Ocean Environment Dynamics, Hangzhou 310012, China;
5. Zhejiang Key Laboratory of Nearshore Engineering Environment and Ecological Security, Hangzhou 310012, China

Received:2022-06-07 Online:2022-12-15 Published:2023-02-09

摘要/Abstract

摘要： 碱性磷酸酶活性(alkaline phosphatase activity, APA)是海洋研究中用于反映浮游植物磷限制状态的重要指标。在长江口等“氮过剩”海域,磷是控制海域初级生产力水平的主要因子,然而,磷限制的范围常常难以界定,当前对不同粒级浮游植物的磷限制效应所知甚少。该文根据2020年夏季长江口航次资料,给出了海洋表层各粒级浮游植物(Net:≥20 μm;Nano:2~20 μm;Pico:0.8~2 μm)APA、浮游细菌APA(0.2~0.8 μm)和溶解态APA(<0.2 μm)的空间分布特征,并分析了APA与环境要素间的相关性。结果显示,各粒级浮游植物APA均与无机磷酸盐浓度(dissolved inorganic phosphate, DIP)呈显著负相关,这表明DIP浓度是影响浮游植物APA分布的主要因素。在平面分布上,各粒级浮游植物APA在近口门的光限制区均较低,且呈现自口门向外逐渐升高的趋势,与DIP的分布特征相反。Net和Nano级APA[平均值分别为 (40.28±32.35) nmol/(L·h)和(52.38±34.78) nmol/(L·h)]显著高于Pico级APA[平均值为(28.43±20.23) nmol/(L·h)],这表明大粒级浮游植物可能更易受DIP下降的影响。该研究中,诱导浮游植物APA快速升高的DIP浓度为0.159 μmol/L,与近岸区磷限制经验阈值相接近。该研究揭示了夏季不同粒级浮游植物对长江口磷分布的响应特征,有助于理解长江口初级生产过程的环境调控机制。

关键词: 长江口, 磷限制, 不同粒级浮游植物, 碱性磷酸酶活性

Abstract: Alkaline phosphatase activity (APA) is an important indicator of phytoplankton phosphorus limitation status in the marine science study. In the “nitrogen-excess” areas such as the Changjiang Estuary (CJE), phosphorus is a major factor controlling the primary productivity in the sea. However, the extent of phosphorus limitation is often difficult to define and little is known about the effect of phosphorus limitation on phytoplankton at different sizes. In this study, the spatial distribution of phytoplankton (Net: ≥20 μm; Nano: 2~20 μm; Pico: 0.8~2 μm) APA, bacterioplankton APA (0.2~0.8 μm) and dissolved APA (<0.2 μm) in the surface layer of the CJE in the summer of 2020 were given, and the correlation between APA and environmental factors were analyzed. The results showed that size-fractionated phytoplankton APA was negatively correlated with dissolved inorganic phosphate (DIP), indicating that DIP concentration was the main factor affecting the distribution of size-fractionated phytoplankton APA. In the spatial distribution, phytoplankton APA was lower in the light-limitation zone near the mouth, and showed an increasing trend from the mouth to the east, contrary to the distribution of DIP. Net and Nano phytoplankton APA (mean values of (40.28±32.35) nmol/(L·h) and (52.38±34.78) nmol/(L·h), respectively) were significantly higher than Pico phytoplankton APA (mean values of (28.43±20.23) nmol/(L·h)) in the CJE, implying that large size phytoplankton were more susceptible to DIP decline. In this study, the DIP concentration for inducing a rapid increase in phytoplankton APA was 0.159 μmol/L, which was close to the empirical threshold for nearshore phosphorus limitation. This study revealed the characteristics of phytoplankton response to phosphorus distribution in the CJE at different grain levels in summer, and also contributed to the understanding of the environmental regulatory mechanisms of primary production processes in the CJE.

Key words: Changjiang Estuary, phosphorus limitation, size-fractionated phytoplankton, alkaline phosphatase activity

中图分类号:

P734

王奕恒, 郝锵, 陈建芳, 朱元励, 金海燕, 周锋, 张伟. 夏季长江口不同粒级浮游植物碱性磷酸酶活性[J]. 海洋学研究, 2022, 40(4): 25-37.

WANG Yiheng, HAO Qiang, CHEN Jianfang, ZHU Yuanli, JIN Haiyan, ZHOU Feng, ZHANG Wei. Alkaline phosphatase activity of size-fractionated phytoplankton in the Changjiang Estuary in summer[J]. Journal of Marine Sciences, 2022, 40(4): 25-37.

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夏季长江口不同粒级浮游植物碱性磷酸酶活性

Alkaline phosphatase activity of size-fractionated phytoplankton in the Changjiang Estuary in summer

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