Antibacterial activity of the coelomic fluid from a marine worm, Phasolosma esculenta

doi:10.3969/j.issn.1001-909X.2015.02.010

Abstract

Abstract: Phasolosma esculenta is a peculiar edible marine economic species in China. In this study, the antimicrobial effect and physical and chemical characteristics of coelomic fluid extracted from Phasolosma esculenta was examined with agar diffusion and plate counting method. The result shows that this fluid is found to have an antibacterial activity against three G⁺ bacteria (Staphylococcus aureus, etc), but has no obvious antibacterial activity against three G^- bacteria (Escherichia coli, etc) and the yeast Candida lusitaniae. Results also show that: (1) Different extraction procedure shows different antibacterial activity. Three extraction procedures are compared: (a) the coelomic fluid without any treatment; (b) with ultra sonication and (c) mixed with 2% acetic acid. Pretreated with an ultra-sonication step, the extract has the best antibacterial effect. (2) The antibacterial activity of this component is effective up to 90 minutes and freezing-resistant. With its optimal temperature of 25 ℃, it shows an antibacterial effect within temperature ranging from 5 to 30 ℃. The fluid is inactivated with water bath treatment at 60 ℃ for 10 minutes. In addition, the antibacterial activity is independent from the metal ions. (3) Moreover, by separating fluid components based on molecular weights, it shows that the effective component is larger than 100 kDa.

Key words: Phasolosma esculenta, coelomic fluid, antibacterial components, physical and chemical characteristics, preliminary separation

CLC Number:

S917.4

LIANG Qian-rong, YU Shu-guang, LIU Shun, WANG Guo-liang, ZHENG Xiao-ye, PENG Xue-mei-hui, ZHOU Su-ming. Antibacterial activity of the coelomic fluid from a marine worm, Phasolosma esculenta[J]. Journal of Marine Sciences, 2015, 33(2): 70-75.

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