宁波-舟山港口航道3种致病性细菌毒力特征与耐药性

doi:10.3969/j.issn.1001-909X.2015.04.009

海洋学研究 ›› 2015, Vol. 33 ›› Issue (4): 77-82.DOI: 10.3969/j.issn.1001-909X.2015.04.009

宁波-舟山港口航道3种致病性细菌毒力特征与耐药性

管峰^1,2, 高威¹, 陈吉刚^1,2, 毛芝娟^1,2, 杨季芳^*1,2

1.浙江万里学院生物与环境学院浙江宁波 315100;
2.宁波市微生物与环境工程重点实验室浙江宁波 315100

收稿日期:2015-05-08 修回日期:2015-05-28 出版日期:2015-12-15 发布日期:2022-11-28
通讯作者: *杨季芳(1963—),男,研究员,主要从事海洋微生物(病毒)生态过程、病原微生物病原病理学研究。E-mail:jfkwlq@163.com
作者简介:管峰(1985—),男,江苏淮安市人,实验师,主要从事环境病原微生物研究。E-mail:guanfengxy@163.com
基金资助:
国家海洋公益性行业科研专项项目资助(201105007-7-Ⅱ)

Virulence characteristics and antimicrobial susceptibility of three pathogenic bacteria isolated from the channels and ports in Ningbo and Zhoushan

GUAN Feng^1,2, GAO Wei¹, CHEN Ji-gang^1,2, MAO Zhi-juan^1,2, YANG Ji-fang^*1,2

1. Faculty of Biological & Environmental Science, Zhejiang Wanli University, Ningbo 315100, China;
2. Municipal Key Laboratory of Microorganism and Environmental Engineering, Ningbo 315100, China

Received:2015-05-08 Revised:2015-05-28 Online:2015-12-15 Published:2022-11-28

摘要/Abstract

摘要： 本文调查分析了宁波-舟山港口航道副溶血弧菌 Vibrio parahemolyticus、溶藻弧菌Vibrio alginolyticus和嗜水气单胞菌 Aeromonas hydrophila的毒力特征和耐药性特点。用PCR方法检测分离菌株的毒力基因分布模式发现,副溶血弧菌毒力基因型以gyrB⁺,toxR⁺,tlh⁺和tdh⁺为代表,溶藻弧菌毒力基因型以aspA⁺,tlh⁺,collagenase⁺和toxS⁺为代表,嗜水气单胞菌毒力基因型以aerA⁺,alt⁺,aha1⁺和hlyA⁺为代表;副溶血弧菌、溶藻弧菌和嗜水气单胞菌代表菌株相应基因的检出率分别为57.1%、40.0%和50.0%。用24种常见抗生素对3种细菌的分离代表菌株进行药敏调查,耐药率分别为4.17%、8.33%和29.17%。实验结果表明宁波-舟山港口航道3种致病性细菌具备较丰富的毒力基因种类和耐药性,需要有针对性地制定预防措施。此外,鉴于毒力基因型与致病性之间的相关性,毒力基因可作为标记基因,用于致病性细菌的检测。

关键词: 港口航道, 致病菌, 毒力基因, 耐药性

Abstract: Virulence characteristics and antimicrobial susceptibility of Vibrio parahaemolyticus(VP), Vibrio alginolyticus(VA) and Aeromonas hydrophila(AH) isolated from the channels and ports in Ningbo and Zhoushan were investigated. The distribution patterns of virulent genes in isolated pathogenic bacteria are found that VP is represented by tlh⁺, toxR⁺, gyrB⁺and tdh⁺; VA is represented by aspA⁺, tlh⁺, collagenase⁺and toxS⁺; AH is represented by aerA⁺, alt⁺, aha1⁺and hlyA⁺. The detection rates of relevant genes in representative strains for these three kinds of bacteria are 57.1%, 40.0%, 50.0%. 24 common kinds of drug resistance rates of representative strains are 4.17%, 8.33% and 29.17% respectively. The results show that harmful characteristics of the pathogenic bacteria are found in Ningbo-Zhoushan, and the right defense measures needed to make to prevent them. Moreover, the correlation of virulence genotypes and pathogenicity of the bacteria show that virulent genes can be chosen as gene-markers to detect pathogenic strains of bacteria.

Key words: channels and ports, pathogenic bacteria, virulent gene, antimicrobial susceptibility

中图分类号:

Q938.8

管峰, 高威, 陈吉刚, 毛芝娟, 杨季芳. 宁波-舟山港口航道3种致病性细菌毒力特征与耐药性[J]. 海洋学研究, 2015, 33(4): 77-82.

GUAN Feng, GAO Wei, CHEN Ji-gang, MAO Zhi-juan, YANG Ji-fang. Virulence characteristics and antimicrobial susceptibility of three pathogenic bacteria isolated from the channels and ports in Ningbo and Zhoushan[J]. Journal of Marine Sciences, 2015, 33(4): 77-82.

参考文献

[1] MESSI P, GUERRIERI E, BONDI M. Antibiotic resistance and antibacterial activity in heterotrophic bacteria of mineral water origin [J]. The Science of the Total Environment,2005,346(1-3):213-219.
[2] BHUPATHIRAJU V K, MCINERNEY M J, WOESE C R, et al. Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine [J]. International Journal of Systematic Bacteriology,1999,49(3):953-960.
[3] JANDA J M, ABBOTT S L. The genus Aeromonas: taxonomy, pathogenicity, and infection[J]. Clinical Microbiology Reviews,2010,23(1):35-73.
[4] NAKA H, DIAS G M, THOMPSON C C, et al. Complete genome sequence of the marine fish pathogen Vibrio anguillarum harboring the pJM1 virulence plasmid and genomic comparison with other virulent strains of V. anguillarum and V. ordalii [J]. Infection and Immunity,2011,79(7):2 889-2 900.
[5] ADKINS J P, MADIGAN M T, MANDELCO L, et al. Arhodomonas aquaeolei gen. nov., sp. nov., an aerobic, halophilic bacterium isolated from a subterranean brine[J]. International Journal of Systematic Bacteriology,1993,43(3):514-520.
[6] TARDY-JACQUENOD C, MAGOT M, PATEL B K, et al. Desulfotomaculum halophilum sp. nov., a halophilic sulfate-reducing bacterium isolated from oil production facilities [J]. International Journal of Systematic Bacteriology,1998,48(2):333-338.
[7] LIN Ji-can, YANG Ze, WU Bo-ci, et al. Investigation of ship ballast water of entry or exit of pathogenic Vibrio[J]. Modern Preventive Medicine,2005(7):761-762,767.
林继灿,杨泽,吴博慈,等.进出境船舶压舱水致病性弧菌的调查[J].现代预防医学,2005(7):761-762,767.
[8] YE Li-qing, YANG Ze, LIN Ji-can, et al. Investigation on the distribution of pathogenic Vibrio in ballast water of ships in port of Zhuhai[J]. Modern Preventive Medicine,2005(3):225-226.
叶立青,杨泽,林继灿,等.珠海港船舶压舱水中致病性弧菌分布的调查[J].现代预防医学,2005(3):225-226.
[9] HU Qun, HAN hui, LEI Lei, et al. Identification and molecular subtyping of Vibiro Vulnificus introduced in ballast water[J]. Chinese Journal of Frontier Health and Quarantine,2012(2):118-120.
胡群,韩辉,雷磊,等.海港口岸输入性创伤弧菌分离鉴定与分子分型研究[J].中国国境卫生检疫杂志,2012(2):118-120.
[10] XIE Z Y, HU C Q, ZHANG L P, et al. Identification and pathogenicity of Vibrio ponticus affecting cultured Japanese sea bass, Lateolabrax japonicus (Cuvier in Cuvier and Valenciennes) [J]. Letters in Applied Microbiology,2007,45(1):62-67.
[11] YIN Qi, SONG Ding-bo, ZOU Xing-lin. Investigation of bacterial hygiene in hands of employees in Zhuhai Jiuzhou Port [J]. Science of Travel Medicine,1998(1):41-42.
尹琦,宋定波,邹醒林.珠海九州港口岸地区食品从业人员双手细菌卫生学情况调查[J].旅行医学科学,1998(1):41-42.
[12] LI Jun-cheng, NIE Wei-zhong, LI De-xin, et al. Investigation report on biological monitoring of ship ballast water of international voyage[J]. Chinese Journal of Frontier Health and Quarantine,2003(S1):46-48.
李俊成,聂维忠,李德昕,等.国际航行船舶压舱水生物监测调查报告[J].中国国境卫生检疫杂志,2003(S1):46-48.
[13] JIA Jun-tao, LI Wei-cai, ZHAO Li-qing, et al. Hierarchical clustering analysis of bacterial composition in ballast water on ships in shangdong main harbors[J]. Marine Environmental Science,2010(4):541-544.
贾俊涛,李伟才,赵丽青,等.山东主要港口入境船舶压载水中细菌组成的等级聚类分析[J].海洋环境科学,2010(4):541-544.
[14] FENG Yun-xiao, ZHANG Le, FANG Zhen-dong, et al. Investigation of microbe taken by ballast water of entry ships in Qinhuangdao port[J]. Environmental Science Survey,2011(2):58-61.
冯云霄,张乐,方振东,等.秦皇岛口岸入境船舶压载水中微生物携带情况调查[J].环境科学导刊,2011(2):58-61.
[15] LI Xiao-hong, LIU Wen-zheng, YANG Yan-qiu, et al. Analysis on invasion risk of pathogenic microorganisms in ship's ballast water at Yantian seaport[J]. Chinese Journal of Frontier Health and Quarantine,2013(3):179-184,191.
李小洪,刘文正,杨燕秋,等.盐田港船舶压载水致病微生物入侵风险分析[J].中国国境卫生检疫杂志,2013(3):179-184,191.
[16] ZHAO Li-qing, JIA Jun-tao, JIANG Ying-hui, et al. MDS analysis on the pathogenic bacterias in Shandong mian Harbor water and entry ship ballast water [J]. Chinese Journal of Frontier Health and Quarantine,2013(5):334-337.
赵丽青,贾俊涛,姜英辉,等.山东主要港口海域及入境船舶压舱水中病原菌调查结果的多维尺度分析[J].中国国境卫生检疫杂志,2013(5):334-337.
[17] DONG Xiu-zhu, CAI Miao-ying. Systematic determinative manual of general bacteria [M]. Beijing:Science Press,2001:192-245.
东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:192-245
[18] BOONE D R, GARRITY G,CASTENHOLZ R W. Bergey's manual of systematic bacteriology[M]. second edition. Springer Verlag,2004:474-491.
[19] WU Ling, ZHANG Jing-long, NI Yu-xing, et al. Study and investigation on the distribution of pathogenic Vibrio of the five coastal provinces in China[J]. Chinese Journal of Epidemiology,1998(5):46-48.
吴玲,张景隆,倪语星,等.我国沿海五省市致病性弧菌的分布调查及病原学研究[J].中华流行病学杂志,1998(5):46-48.
[20] ROQUE A, MOLINA-AJA A, BOLAN-MEJIA C, et al. In vitro susceptibility to 15 antibiotics of vibrios isolated from penaeid shrimps in Northwestern Mexico[J]. International Journal of Antimicrobial Agents,2001,17(5):383-387.
[21] HU Long-yu, NING Xi-bin, JIA Ling-hua. Isolation,identification and antimicrobial resistance research of Vibrio alginolyticus isolated from seawater[J]. Science and Technology of Food Industry,2013(16):195-199.
胡珑玉,宁喜斌,贾玲华.海水中溶藻弧菌的分离、鉴定及其耐药性分析[J].食品工业科技,2013(16):195-199.
[22] NASH G, NITHIMATHACHOKE C, TUNGMANDI C, et al. Vibriosis and its control in pond-reared Penaeus monodon in Thailand[C]//SHARIFF M, SUBASINGHE R P, ARTHUR J R. Diseases in Asian aquaculture I. Fish health section. Manila: Asian Fisheries Society,1992:143-155.
[23] KABIR S, MANN P. Immunological properties of the cell envelope components of Vibrio cholerae [J]. Journal of General Microbiology,1980,119(2):517-525.
[24] MARTINSEN B, OPPEGAARD H, WICHSTROM R, et al. Temperature-dependent in vitro antimicrobial activity of four 4-quinolones and oxytetracycline against bacteria pathogenic to fish[J]. Antimicrobial Agents and Chemotherapy,1992,36(8):1 738-1 743.

宁波-舟山港口航道3种致病性细菌毒力特征与耐药性

Virulence characteristics and antimicrobial susceptibility of three pathogenic bacteria isolated from the channels and ports in Ningbo and Zhoushan

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