Journal of Marine Sciences ›› 2023, Vol. 41 ›› Issue (3): 92-100.DOI: 10.3969/j.issn.1001-909X.2023.03.009

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Preparation of standard reference material for reactive phosphorus with seawater matrix

ZHANG Chuan1,2(), YU Tao1,2, YU Xiaoyan1,2, ZHU Yong3,4, WANG Lifang5, ZHANG Xiaohui1,2   

  1. 1. National Center of Ocean Standards and Metrology, Tianjin 300112, China
    2. Technology Innovation Center for Marine Metrology and Instruments Test, MNR, Tianjin 300112, China
    3. Second Institute of Oceanography, MNR, Hangzhou 310012, China
    4. Key Laboratory of Marine Ecosystem Dynamics, MNR, Hangzhou 310012, China
    5. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China
  • Received:2023-01-19 Revised:2023-03-18 Online:2023-09-15 Published:2023-10-24


Based on Western Pacific surface seawater as matrix, a series of reactive phosphorus reference materials with the concentration of 0.5-4.0 μmol/L were prepared. By investigating the uniformity and stability of the 4 batches of samples, it was confirmed that the performance met the needs of requirements. For the determination of reactive phosphorus in aqueous sample, segmented continuous flow analysis system with UV detector was used. The experimental conditions were optimized, the limit of detection(LOD) of the method was 0.03 μmol/L, and the relative standard deviation (RSD) of 4 different concentration batches was 0.37%-2.45%. The reliability of the method was verified by testing a CRM. The error was not more than 0.05 μmol/L. The concentration standard value of the series of reference substances was determined by 6 laboratories, and the relative expended uncertainty of characterization was evaluated, which was 2%-10%.

Key words: seawater matrix, reactive phosphorus, reference material

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