基于正交实验的高含量藻红蛋白紫菜丝状藻体优化调控培养条件研究

doi:10.3969/j.issn.1001-909X.2014.02.009

摘要/Abstract

摘要： 丝状藻体在紫菜养殖的人工苗种生产中非常重要,在利用于分离提取高价值的藻红蛋白方面也具有潜在的利用价值。本研究采集了成熟紫菜叶状体的果孢子释放的初始丝状藻体,基于3因子3水平的正交实验设计方案[采用具有分析交互作用的正交表L₂₇(3¹³)],确定了丝状藻体藻红蛋白含量对环境因子的响应以及优化的调控培养条件。结果表明,温度、光照强度和盐度因子对丝状藻体藻红蛋白含量均有显著影响(P≤0.048 1),并且温度和光照强度或盐度因子间存在交互作用(P ≤0.043 1),但光照强度和盐度因子间的交互作用不显著(P=0.469 8)。获得的优化调控培养条件为:温度18 ℃、光强1 500 lx、盐度20或25。优化调控培养条件下的丝状藻体藻红蛋白含量高达64.73 mg/g.dw(平均值±标准偏差SD为58.24±5.07 mg/g.dw)。

关键词: 坛紫菜, 丝状藻体, 藻红蛋白, 正交实验, 温度, 光强, 盐度

Abstract: The conchocelis is very important for artificial seeding production in Porphyra cultivation and can be potentially used for separation and extraction of high value phycoerythrin. We investigated several influential factors related to phycoerythrin content of Porphyra haitanensis conchocelis and studied the optimal controlled culture conditions under which high content of phycoerythrin was obtained. Specific operational processes: initial conchocelis was obtained from carpospores release of mature Porphyra thallus and responses of conchocelis phycoerythrin content to environmental factors and optimal controlled culture conditions were determined based on the designation of an orthogonal experimentation with interactional analysis of three factors and three levels [L₂₇(3¹³)]. Results demonstrate that factors of temperature,light intensity and salinity have significant effects on phycoerythrin content (P≤0.048 1). There are factorial interaction occurrences between temperature and light intensity or salinity(P≤0.043 1). However there is no significant interaction between light intensity and salinity(P=0.469 8). The optimal controlled culture conditions for high content of phycoerythrin are: temperature 18 ℃、light intensity 1 500 lx and salinity 20 or 25. Under optimal controlled culture condition, conchocelis phycoerythrin content is as high as 64.73 mg/g.dw (average±SD: 58.24±5.07 mg/g.dw).

Key words: Porphyra haitanensis, conchocelis, phycoerythrin, orthogonal experimentation, temperature, light intensity, salinity

中图分类号:

林汝榕, 邢炳鹏, 柯秀蓉, 蔡文旋, 林锡煌, 张稚兰. 基于正交实验的高含量藻红蛋白紫菜丝状藻体优化调控培养条件研究[J]. 海洋学研究, 2014, 32(2): 67-73.

LIN Ru-rong, XING Bing-peng, KE Xiu-rong, CAI Wen-xuan, LIN Xi-huang, ZHANG Zhi-lan. Optimal culture conditions for Porphyra haitanensis conchocelis with high content of phycoerythrin based on the orthogonal experimentation[J]. Journal of Marine Sciences, 2014, 32(2): 67-73.

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