台风过境对养殖三疣梭子蟹抗氧化系统及Na+,K+-ATP酶活性的影响

doi:10.3969/j.issn.1001-909X.2018.03.012

摘要/Abstract

摘要： 为了研究台风过境对养殖三疣梭子蟹的影响,于2015年7月采集“灿鸿”台风经过象山前后12 h内象山三疣梭子蟹养殖场样品,检测台风前后三疣梭子蟹中T-SOD、CAT、ACP、Na⁺,K⁺-ATP酶活性以及MDA、GSH含量的变化。结果显示:台风过后鳃中T-SOD酶活性升高极其显著(p<0.01);CAT酶活性显著升高(p<0.05);MDA和GSH含量则呈现增加趋势,但差异不显著(p>0.05)。肝胰腺中T-SOD酶活性呈现降低趋势,但差异不显著(p>0.05);CAT酶活性呈现升高趋势,差异不显著(p>0.05);MDA含量显著增加(p<0.05);GSH含量增加极其显著(p<0.01)。心脏中T-SOD、CAT酶活性和GSH含量呈现增加趋势而MDA含量呈现降低趋势且差异均不显著(p>0.05)。心脏和鳃中ACP酶活性呈现上升趋势,肝胰腺中ACP酶活性降低,但差异都不显著(p>0.05)。Na⁺,K⁺-ATP酶活性在鳃中极显著下降(p<0.01),在肝胰腺中酶活性极显著上升(p<0.01),在心脏中酶活性显著上升(p<0.05)。结果表明台风会造成三疣梭子蟹短期内应激反应,如若不尽快采取措施,后期会对三疣梭子蟹造成较大的损伤。

关键词: 养殖三疣梭子蟹, 台风, 酶活性

Abstract: In order to study the effect of typhoon on the cultured Portunus trituberculatus, the changes of T-SOD, CAT, MDA, GSH, ACP and Na⁺, K⁺-ATPase in the hearts, gills and hepatopancreas were detected in 12 hours before and after typhoon “Canhong”(1509) at Xiangshan in July, 2015. The typhoon increased highly significantly the activity of the T-SOD enzyme (p<0.01) and increased significantly CAT enzyme(p<0.05) in the gills. After typhoon, the MDA and GSH content in gills increased insignificantly (p>0.05). T-SOD enzyme activity in the hepatopancreas showed an insignificant trend of decrease (p>0.05) while the CAT enzyme activity showed an insignificant trend of increase (p>0.05), MDA content increased significantly (p<0.05) and the increase of the GSH content was highly significant (p<0.01). In the hearts, the levels of T-SOD, CAT enzyme activity and GSH content increased and the MDA content decreased, but these changes were not significant(p>0.05). In addition, the ACP enzyme activity in the gills and the hearts increased insignificantly(p>0.05), which was reduced but not significantly in the hepatopancreas (p>0.05). The Na⁺, K⁺-ATPase activity in the gills decreased highly significantly (p<0.01), but increased highly significantly in the hepatopancreas (p<0.01) and significantly in the heart (p<0.05) . This study reveals that the typhoon can cause the cultured P. trituberculatus stress response in the short term and solutions are need to take to avoid typhoon damage to cultured P. trituberculatus.

Key words: cultured Portunus trituberculatus, typhoon, enzyme activity

中图分类号:

S968.25

陈夏月, 任永宽, 李连君, 母昌考, 李荣华, 宋微微, 王春琳. 台风过境对养殖三疣梭子蟹抗氧化系统及Na⁺,K⁺-ATP酶活性的影响[J]. 海洋学研究, 2018, 36(3): 101-106.

CHEN Xia-yue, REN Yong-kuan, LI Lian-jun, MU Chang-kao, LI Rong-hua, SONG Wei-wei, WANG Chun-lin. Effect of typhoon on the antioxidant system and Na⁺, K⁺-ATPase activity in Portunus trituberculatus[J]. Journal of Marine Sciences, 2018, 36(3): 101-106.

参考文献

[1] SHI Hui-lai, JIN Chong-lue, LIN Gui-zhuang, et al. Breeding status of Portunus trituberculatus in Zhejiang[J]. Hebei Fisheries, 2010, 38(7) : 39-41.
史会来, 金翀略, 林桂装, 等. 浙江三疣梭子蟹养殖现状[J]. 河北渔业, 2010, 38(7) : 39-41.
[2] XU Wen-ling. The impact of the typhoon on sea surface temperature[D]. Qingdao: Ocean University of China, 2007.
徐文玲. 台风对海表温度的影响[D] . 青岛:中国海洋大学, 2007.
[3] ZHENG Shan-jian, WANG Fang. Effects of typhoon on water quality of litopenaeus vannamei desalination Pond[J]. Reservoir Fisheries, 2007, 27(1): 49-50,100.
郑善坚, 王芳. 台风对凡纳滨对虾淡化养殖池水质的影响[J]. 水利渔业, 2007, 27(1): 49-50,100.
[4] WEI Man-xin, HE Ben-mao, TONG Wan-ping. The effect of tyhpoon to the water environmental factor of a close indoor shrimp pond in industrialized cultivationin ecological system[J]. Journal of Guangxi Academy of Sciences, 2006, 22(2):70-74.
韦蔓新, 何本茂, 童万平. 台风对封闭式工厂化虾池生态系统中水环境因子的影响[J]. 广西科学院学报, 2006, 22(2): 70-74.
[5] PENG Rong-nan, YANG Jie-yan, WU Nai-geng. Study on water quality mutation of shrimp farms induced by meteorological factors in typhoon[J] . Guangdong Meteorology, 2016, 38(1): 66-69,72.
彭荣南, 杨杰颜, 吴乃庚. 台风过程气象因子引发的对虾养殖场水质突变分析[J]. 广东气象, 2016, 38(1): 66-69,72.
[6] GAO Bin, GAO Hong. Oxygen free radical and cell injury[J]. Progress in Veterinary Medicine, 2002, 23(5): 34-36.
高斌, 高洪. 氧自由基与细胞损伤[J]. 动物医学进展, 2002, 23(5): 34-36.
[7] FANG Chun-hua, QIAO Kun, LIU Zhi-yu, et al. The research progress of antioxidant enzymes in marine organisms[J]. Fishery Research, 2016, 38(4): 331-342.
方春华, 乔琨, 刘智禹, 等. 海洋生物中抗氧化酶的研究进展[J]. 渔业研究, 2016, 38(4): 331-342.
[8] CHEN Hai-guang, YE Dan-jie. Oxygen free radicals and their scavengers[J]. Journal of Zhongkai University of Agriculture and Technology, 2002, 15(1): 53-60.
陈海光, 叶丹杰. 氧自由基及其清除剂[J]. 仲恺农业技术学院学报, 2002, 15(1): 53-60.
[9] SHI Qin-feng, CAO Wei-rong. Studies on the study of superoxide dismutase (SOD)[J]. Livestock and Poultry Industry, 2009, 20(4): 66-68.
时沁峰, 曹威荣. 超氧化物歧化酶(SOD)的研究概况[J]. 畜禽业, 2009, 20(4): 66-68.
[10] LIU Ling-zhi, ZHONG Guang-rong, XIONG Lian, et al. Research and application progress of catalase[J]. Chemistry & Bioengineering, 2009, 26(3): 15-18.
刘灵芝, 钟广蓉, 熊莲, 等. 过氧化氢酶的研究与应用新进展[J]. 化学与生物工程, 2009, 26(3): 15-18.
[11] JIA Zhen, WANG Dan, YOU Song. Progress on glutathione and its preparation.[J]. Journal of Shenyang Pharmaceutical University, 2009,26(3): 238-242.
贾贞, 王丹, 游松. 谷胱甘肽的研究进展[J]. 沈阳药科大学学报, 2009, 26(3): 238-242.
[12] GUILFORD G.Management of ischemic diseases[J]. Publication Veterinary Continuing Education, Massey University, 1994, 156:48-56.
[13] ZHAO Ke-ran. Oxygen free radical and clinical[M].Beijing: China Medical Science Press, 2000: 44-51.
赵克燃. 氧自由基与临床[M]. 北京: 中国医药科技出版社,2000: 44-51.
[14] ZHOU Shuang-lin, JIANG Nai-cheng, LU Jian-pin, et al. Advances in osmotic pressure regulation of crustaceansⅠ. Structure and function of gills and its influencing factors[J]. Donghai Marine Science, 2001,19(1): 44-51.
周双林, 姜乃澄, 卢建平, 等. 甲壳动物渗透压调节的研究进展Ⅰ.鳃的结构与功能及其影响因子[J]. 东海海洋, 2001, 19(1): 44-51.
[15] ZHANG Long-gang, DONG Xue-sa, ZHU Yong-an, et al. Effects of acute salinity stress on antioxidant enzymes and Na⁺, K⁺-ATPase activities of gill in Procambarus clarkii[J]. Freshwater Fisheries, 2015, 45(4): 87-91,112.
张龙岗, 董学飒, 朱永安, 等. 急性盐度胁迫对克氏原螯虾肝脏抗氧化酶及鳃丝Na⁺, K⁺-ATP酶活力的影响[J]. 淡水渔业, 2015, 45(4): 87-91,112.
[16] GUO Qin-dan, WANG You-ji, LÜ Wei-qun. Combined effects of temperature and salinity on the physiological osmotic induction and antioxidant responses in the juvenile japanese flounder (Paralichthys olivaceus)[J]. Acta Hydrobiologica Sinica, 2014, 38(1): 58-67.
郭勤单, 王有基, 吕为群. 温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响[J]. 水生生物学报, 2014, 38(1): 58-67.
[17] ZHANG Chen-jie, ZHANG Yan-liang, GAO Quan-xin, et al. Effects of low salt stress on antioxidant function of young Spotted Maigre[J]. South China Fisheries Science, 2015, 11(4): 59-64.
张晨捷, 张艳亮, 高权新, 等. 低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响[J]. 南方水产科学, 2015, 11(4): 59-64.
[18] YAO Zhi-feng, ZHANG Long-zhen, ZHUANG Ping, et al. Effects of antioxidant enzyme in liver and acute toxicity of Cu²⁺ on juvenile Chinese sturgeon[J]. Journal of Fishery Sciences of China, 2010, 17(4): 731-738.
姚志峰, 章龙珍, 庄平, 等. 铜对中华鲟幼鱼的急性毒性及对肝脏抗氧化酶活性的影响[J]. 中国水产科学, 2010, 17(4): 731-738.
[19] CHEN Yin-er, WANG Guo-liang, JIN Shan, et al. Enzyme activities in Portunus trituberculatus with emulsification disease [J]. Fisheries Science, 2006, 25(9): 448-451.
陈寅儿,王国良,金珊,等. 三疣梭子蟹患“乳化病”后几种保护酶活力的变化[J]. 水产科学, 2006, 25(9): 448-451.
[20] YIN Fei, SUN Peng, PENG Shi-ming, et al. Effects of low salinity stress on the antioxidant enzyme activities in juvenile Pampus argenteus liver and the APTase activities in its gill and kidney.[J]. Chinese Journal of Applied Ecology, 2011, 22(4): 1 059-1 066.
尹飞, 孙鹏, 彭士明, 等. 低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响[J]. 应用生态学报, 2011, 22(4): 1 059-1 066.
[21] LIU Xiao-hua. Antioxidative defence mechanism of Glutathione on Litopenaeus vannamei[D]. Wuhan: Huazhong Agricultural University, 2010.
刘晓华. 谷胱甘肽对凡纳滨对虾抗氧化防御的调控机理[D]. 武汉: 华中华业大学, 2010.
[22] LI Li-hong, YUAN Hong-li, HU Zhen-ping, et al. Response of Glutathione in Carp(Cyprinus carpio L.) to Ammonia stress [J]. Journal of Anhui Agricultural Sciences, 2015, 43(26): 130-131.
李利红, 袁宏利, 胡振平, 等. 黄河鲤谷胱甘肽对氨氮胁迫的生理响应[J]. 安徽农业科学, 2015, 43(26): 130-131.
[23] WEI Ke-qiang, XU Zi-rong. Studies on immune mechanism in shrimp and strategies for disease prevention[J]. Chinese Journal of Veterinary Drugs, 2004, 38(9): 25-29.
魏克强, 许梓荣. 对虾的免疫机制及其疾病预防策略的研究[J]. 中国兽药杂志, 2004, 38(9): 25-29.
[24] CHENG T C. The role of lysosomal hydrolases in molluscan cellular response to immunologic challenge[J]. Comp Pathobiol, 1978(4): 59-71.
[25] OU You-jun, LUO Qi, LI Jia-er. Distribution and cryopreservation of alkaline phosphatase(AKP) and acid phosphatase(ACP) in Trachinotus ovatus[J]. Southern Fisheries Science, 2011, 7(2): 49-54.
区又君, 罗奇, 李加儿. 卵形鲳鲹碱性磷酸酶和酸性磷酸酶的分布及其低温保存[J]. 南方水产科学, 2011, 7(2):49-54.
[26] ZHOU Dong, MU Chang-kao, SONG Wei-wei, et al. Effects of low salinity stress on the antioxidant enzyme and ATPase activities intissues of swimming crab Portunus trituberculatus[J]. Ecologic Science, 2014, 33(4):698-703.
周东, 母昌考, 宋微微, 等. 低盐胁迫对三疣梭子蟹组织中抗氧化酶和ATP酶活力的影响[J]. 生态科学, 2014, 33(4): 698-703.
[27] JIANG Shan, XU Qiang-hua. Influence of salinity stress on the activity of gill Na⁺/K⁺-ATPase in swimming crab (Portunus trituberculatus)[J]. Journal of Fisheries of China, 2011, 35(10): 1 475-1480.
江山, 许强华. 盐度胁迫对三疣梭子蟹鳃Na⁺/K⁺-ATPase酶活的影响[J]. 水产学报, 2011, 35(10) : 1 475 -1 480.
[28] ZHAO Feng, ZHUANG Pin, ZHANG Long-zhen, et al. The influence of salinity acclimation on activity of Na⁺/K⁺-ATPase in branchial epithelium, concentration of ions and osmolarity in serum of Acipenser schrenckii[J]. Journal of Fisheries of China, 2006, 30(4): 444-449.
赵峰, 庄平, 章龙珍, 等. 盐度驯化对史氏鲟鳃Na⁺/K⁺-ATP酶活力、血清渗透压及离子浓度的影响[J]. 水产学报, 2006, 30(4): 444-449.
[29] WAN Yan, SU Pin. Na⁺, K⁺-ATPase:the important factor influencing on sperm movement[J]. Reproduction & Contraception, 2009, 29(8): 537- 542 .
万艳, 苏萍. Na⁺/K⁺-ATP酶——影响精子运动的重要因素[J]. 生殖与避孕, 2009, 29(8) : 537-542.