海洋学研究 ›› 2024, Vol. 42 ›› Issue (1): 91-105.DOI: 10.3969/j.issn.1001-909X.2024.01.009

• 研究论文 • 上一篇    下一篇

海洋赤杆菌科菌株的类胡萝卜素合成基因分析

岳小岚1,2(), 徐林2,3, 符格意2, 许学伟2,*()   

  1. 1.上海交通大学 海洋学院,上海 200240
    2.自然资源部第二海洋研究所,自然资源部海洋生态系统动力学重点实验室,浙江 杭州 310012
    3.浙江理工大学 生命科学与医药学院,浙江 杭州 310018
  • 收稿日期:2023-04-04 修回日期:2023-05-16 出版日期:2024-03-15 发布日期:2024-05-11
  • 通讯作者: * 许学伟(1978—),男,研究员,主要从事海洋微生物资源方面的研究,E-mail:xuxw@sio.org.cn。
  • 作者简介:岳小岚(1998—),女,陕西省汉中市人,主要从事生物海洋学与海洋生物学方面的研究,E-mail:x1412519@sjtu.edu.cn。
  • 基金资助:
    国家自然科学基金项目(32170005);国家自然科学基金项目(32000001)

Investigation of carotenoid biosynthesis genes in marine Erythrobacteraceae strains

YUE Xiaolan1,2(), XU Lin2,3, FU Geyi2, XU Xuewei2,*()   

  1. 1. School of Oceanography, Shanghai Jiao Tong University, Shanghai 200240, China
    2. Second Institute of Oceanography, Key Laboratory of Marine Ecosystem Dynamics, MNR, Hangzhou 310012, China
    3. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
  • Received:2023-04-04 Revised:2023-05-16 Online:2024-03-15 Published:2024-05-11

RichHTML

4

PDF

25

摘要：

赤杆菌科(Erythrobacteraceae)菌株广泛分布于海洋环境,可合成类胡萝卜素等多种色素。类胡萝卜素具有光保护和抗氧化能力,对于赤杆菌科菌株适应海洋生态系统具有重要作用。本研究收集了107个海洋来源的赤杆菌科细菌基因组,分析了类胡萝卜素合成途径中关键酶的编码基因crtEBIGYZW在基因组中的分布特征,构建了基于氨基酸序列的不同编码基因系统发育树,阐明了深海和浅海来源的赤杆菌科菌株中类胡萝卜素合成基因的存在和分布规律,探究了不同的类胡萝卜素合成基因在近海与深海来源的赤杆菌科菌株进化过程中的遗传模式。研究结果显示,海洋来源的赤杆菌科菌株均含有crtEIGZ基因,约98.1%的菌株含有crtBY基因,约43.9%的菌株中存在crtW基因,其类胡萝卜素合成基因在菌株间存在分布差异,但在深海与浅海来源上并未显示特异性。此外,通过比较不同编码基因的系统发育树拓扑结构发现,赤杆菌科菌株的类胡萝卜素合成基因crtBYZW与系统发育密切相关,而crtEIG基因多通过水平基因转移获得,这有助于更好地评估类胡萝卜素合成基因家族在赤杆菌科中的进化,也为其他海洋细菌中的类胡萝卜素合成途径及基因研究提供科学依据,从而有助于开发海洋来源的产类胡萝卜素菌株。

关键词: 赤杆菌科, 海洋, 类胡萝卜素合成基因, 系统发育, 进化

Abstract:

Erythrobacteraceae is widely distributed in marine environments and can synthesize various pigments such as carotenoids. Carotenoids have photoprotective and antioxidant abilities, and they can play a crucial role in the adaptation of Erythrobacteraceae to marine ecosystems. In this study, we obtained the genomes of 107 marine Erythrobacteriaceae strains, analyzed the distribution characteristics of carotenoid biosynthesis genes including crtEBIGYZW in their genomes, constructed phylogenetic trees based on the amino acid sequences of the carotenoid biosynthesis genes, and elucidated the presence and distribution of carotenoid biosynthesis genes in Erythrobacteriaceae from deep-sea and shallow-sea sources. The results suggested that all of marine Erythrobacteriaceae strains contained the crtEIGZ genes, 98.1% of strains contained the crtBY genes, and 43.9% of them contained the crtW gene, which differed among strains but no any specificities found among the deep-sea or shallow-sea sources. Additionally, in the comparison of the phylogenetic topology, it was found that the crtBYZW genes were vertically transferred from their ancestor, while the crtEIG genes were mainly horizontal gene transferred. Our study aids in the understanding of the carotenoid biosynthesis gene evolution in the family Erythrobacteriaceae and also serves as a scientific basis for the study of carotenoid synthesis pathways and genes in other marine bacteria, enables a thorough understanding of marine carotenoid-synthesizing strains.

Key words: Erythrobacteriaceae, marine, carotenoid biosynthesis gene, phylogeny, evolution

中图分类号: